Advanced Technology Core Laboratories
The Antibody-Based Proteomics Core provides highly customized services to Baylor and the Texas Medical Center community for protein profiling and affinity based proteomic platforms. Services include Luminex bead technology for multiplex quantitative analyses of intracellular and extracellular proteins and various signaling pathways; antibody and protein arrays (including reverse phase protein arrays or RPPA) as discovery and/or validation tools for protein biomarkers, and construction of monoclonal antibody producing B-cell hybridomas and recombinant baculoviruses for expression of proteins in insect cells. For more information, visit the Antibody-Based Proteomics web site.
Director: Shixia Huang, Ph.D.
Phone: (713) 798-8722
Bioinformatics Research Lab
The Bioinformatics Research Laboratory Core provides data hosting and bioinformatics support for applications of massively parallel sequencing, including: transcriptomics, cistromics, epigenomics and metagenomics. The services are provided through Genboree. Go to the Bioinformatics Research Laboratory Core website.
Director: Aleksandar Milosavljevic, Ph.D.
Phone: (713) 798-8719
Cell-Based Assay Screening Service
The C-BASS core makes available to the Baylor community the human and mouse shRNA library collections, through the Open Access Program from Open Biosystems. C-BASS can provide individual shRNA plasmids for use in any Baylor laboratory, or carry out an entire screening experiment using the libraries. C-BASS personnel are available to facilitate the use of these reagents and perform RNAi screens with a level of involvement that is appropriate for the specific experiment. Downstream analyses of the RNAi screen can include, in-house, high-throughput image analysis and flow cytometry. In addition, DNA oligo chip hybridization for the identification of the barcoded shRNA plasmids can be carried out at the Baylor Microarray Core Facility. Go to the Cell-Based Assay Screening Service website.
Directors: Dan Liu, Ph.D. / Trey Westbrook, Ph.D.
Phone: (713) 798-8032
Core for Advanced MRI
The Core for Advanced MRI (CAMRI) provides technology and services for advanced imaging studies of the function, physiology and anatomy of humans and large animals, with a special emphasis on blood-oxygen level dependent functional magnetic resonance imaging (BOLD fMRI) of the human brain. The center houses three Siemens Magnetom Trio Scanners (3 tesla field strength). A full complement of product pulse sequences are available for high resolution structural imaging, diffusion tensor imaging (DTI), fMRI, Arterial Spin Labeling (ASL) and single and multi-voxel magnetic resonance spectroscopy (MRS). Research sequences are available for specialized imaging applications. For functional studies, equipment is available for stimulation of the auditory, visual, somatosensory and gustatory modalities, for infrared eye tracking of subject eye position, and for manual and vocal response recording. Consultation with a physicist is available for pulse sequence optimization and design. Users can scan with a MR technologist, or can elect to obtain training to scan independently. Go to the Core for Advanced MRI (CAMRI) page for more information.
Cytometry and Cell Sorting
The Cytometry and Cell Sorting Core at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analyses and cell sorting. Go to the Cytometry and Cell Sorting Core website.
Directors: Joel M. Sederstrom, M.B.S. / Christine Beeton, Ph.D.
Phone: (713) 798-3868
DNA Sequencing and Gene Vector
The core provides 3 services: DNA sequencing, cDNA clones and Gene Vector. DNA sequencing core provides automated sequencing on Applied Biosystems Model 3130XL Genetic Analyzer. Clone library provides human and mouse cDNA clones as bacterials streaks in agar plates. Gene vector core provides viral vector packaging and cloning/plasmid preparation service. Go to the DNA Sequencing and Gene Vector website.
Academic Director: Benny Hung-Junn Chang, Ph.D.
Phone: (713) 798-6686
Genetically Engineered Mouse
The GEM Core provides Baylor investigators with advice and services requiring the manipulation of mouse gametes to facilitate research involving genetically engineered mice. The GEM Core provides the following services and all can be scheduled and tracked using Mouse Embryo Manipulation Services, MEMS: DNA microinjection (traditional transgene DNA constructs or BAC DNA into the one cell mouse embryo), ES cell microinjection into blastocysts, strain rederivation into a pathogen free strain, and embryo cryopreservation for safe preservation of valuable mouse strains. Go to the Genetically Engineered Mouse website.
Director: Jianming Xu, Ph.D.
Phone: (713) 798-6199
Genomic and RNA Profiling
The Genomic and RNA Profiling Core or GARP (formerly the Microarray Core Facility) provides researchers access to state-of-the-art molecular profiling technology and services. These services include microarray technology platforms (Affymetrix, Agilent, Taqman Low Density Arrays and SuperArray), Next Gen Sequencing (MiSeq Benchtop Sequencer, HiSeq 2500 and HiSeq 2000), NanoString Technology, and Luminex and Panomics bead-based multiplexing technology. This facility also serves as a source for sample quality control, experimental design and comprehensive data analysis. Go to the Genomic Profiling website.
Director: Lisa White, Ph.D.
Phone: (713) 798-7699
Human Stem Cell Core
The Human Stem Cell Core provides a wide range of products and services related to human pluripotent stem cell research, as well as hands-on training classes for basic and advanced stem cell culture techniques. The HSCC offers cost-effective solutions to generate and characterize novel induced pluripotent stem (iPS) cells for in vitro disease modeling, employing “zero footprint” technologies such as Sendai virus-based and episomal vector based reprogramming. The core also offers customized research support for experimental design and validation assays. Go to the Human Stem Cell Core website.
Directors: Margaret Goodell, Ph.D., Academic Director, Jean J. Kim, Ph.D., Core Director
Phone: (713) 798-7471
Human Tissue Acquisition and Pathology
The Human Tissue Acquisition and Pathology Core provides tissue and serum related services to researchers that includes histology and immunohistochemistry and organization of the different tissue banks at BCM. Histology and IHC of human and experimental animal tissues are available to all BCM faculty on a fee-for-service basis. Services include tissue processing, embedding, sectioning and staining for routine histology (H&E, PAS, ORO, Trichrome, VVG, Giemsa), IHC and TUNEL assay for apoptosis. Also available are laser capture microdissection, archival tissue microarrays (TMA), image analysis by Inform and Vectra and consultation with pathologists. Go to the Human Tissue Acquisition and Pathology website.
Director: Michael Ittmann, M.D., Ph.D.
Phone: (713) 798-3705
Fluorescence, confocal, deconvolution and electron microscopy services are available. Instrumentation, available in the Department of Molecular and Cellular Biology, includes a Beckman-Coulter IC-100 (high throughput microscopy and analysis system); Zeiss AxioPlan2 Microscope with CoolSNAP HQ CCD MetaView, MetaMorph; Zeiss LSM 510 META Confocal/Spectral Imaging Microscope; Applied Precision SoftWoRx Image Restoration Microscope (deconvolution); Hitachi H-7500 Transmission Electron Microscope with Gatan 2Kx2K CCD; Silicon Graphics and PC Workstations; RMC Ultramicrotome. Go to the Integrated Microscopy website.
Director: Michael Mancini, Ph.D.
Phone: (713) 798-8952
Mass Spectrometry Proteomics
The Mass Spectrometry-Proteomics Core Laboratory (MS-PCL) provides services for high sensitivity high throughput triple Time-Of-Flight mass spectrometer and full-fledged proteomics technologies for discovery and validation of biomarkers of various diseases. The services include (i) rapid mass determination of proteins and peptides, (ii) identification of proteins/peptides in complex biological samples, (iii) subproteome isolation and characterization, and (iv) detailed analysis of post-translational modification of proteins/peptides. The MS-PCL is also planning to meet the Good Laboratory Practice. Go to the Mass Spectrometry Proteomics website.
Director: Hon-Chiu Eastwood Leung, Ph.D.
Phone: (713) 798-6360
This core provides metabolomics technologies for discovery and validation of biomarkers of various diseases with state-of-the-art high mass spectrometry as the main platform. Core competencies include both targeted assays (up to 500 metabolites) for many analyte classes as well as untargeted assays to evaluate the entire metabolome in biofluids, cell lines and tissues. Services also includes measurement of flux through various pathways using 13C-isotopomer analyses, and structural identification of isolated unknowns. The Biolog Phenotype Microarray system is also available to test for thousands of cellular phenotypes in a multiplex platform. Go to the Metabolomics Core website.
Directors: Arun Sreekumar, Ph.D. / Nagireddy Putluri, Ph.D./Vivekananda Shetty, Ph.D./ Feng Li, Ph.D.
The tetramer production core provides customized MHC/peptide tetramers for identification of antigen-specific T lymphocytes by flow cytometry. Tetramers and/or their derivatives are provided for research purposes only; their use for commercial purposes is prohibited. Go to the MHC Tetramer website.
Director: Xiaochi Lily Wang, M.S.
Phone: (713) 798-3918
Monoclonal Antibody/Recombinant Protein Expression
This core provides custom services for construction of monoclonal antibody producing B-cell hybridomas and recombinant baculoviruses for expression of proteins in insect cells. After construction of hybridomas, the core mass produces and purifies MAbs from large-scale hybridoma cultures grown either in conventional spinner vessels (up to 5.0 liters) or in hollow-fiber bioreactors. Baculovirus recombinant proteins are expressed in either conventional spinner cultures or oxygenated bioreactors. Key core equipment includes 27°C BOD incubators and Applikon 5 liter oxygenated bioreactors for large scale growth of Sf9 insect cells, hollow-fiber bioreactors (FiberCell Systems) for large scale production of hybridomas, and MAbs in vitro and FPLC systems for MAb purification. Go to the Monoclonal Antibody/Recombinant Protein Expression website.
Lab Manager: Kurt Christensen
Phone: (713) 798-2325
Mouse Embryonic Stem Cell Core
The Mouse ES Cell Core generates genetically engineered mice needed by researchers across the United States who are in pursuit of answers and cures for human disease. Core personnel assist investigators with projects that involve generating mutations in the germ line and somatic tissues of mice by performing homologous recombination in ES cells. ES services include electroporation, ROSA targeting, Close Expansion, the generation of primary mouse ES cell lines and Murine Virus Testing. Go to the Mouse Embryonic Stem Cell Core website.
Director: Jason Heaney, Ph.D.
Mouse Metabolic Research Unit (Children's Nutrition Research Center)
The Mouse Metabolic Research Unit at the Children's Nutrition Research Center has extensive facilities for automating feeding studies and for measuring numerous metabolic parameters related to understanding energy expenditure and its various determinants (substrate oxidation, activity, body composition, etc.) in mice (as well as in pigs, although more limited). The number of mouse metabolic units available enables concurrent measurements on a relatively large number of mice. Dedicated engineer and faculty members also available to help investigators design their experiments and interpret their data. Go to the Mouse Metabolic Research Unit website.
Director: Marta Fiorotto, Ph.D.
Phone: (713) 798-7146
The Mouse Metabolism Core (MMC) supports the application of state-of-the-art analysis of metabolic pathways and related physiological and biochemical parameters in mice and rats, as well as in isolated / cultured cells in vitro. Gene targeting and transgenesis have produced a large number of genetic mouse models for metabolism research. The MMC employs sophisticated assays and procedures to characterize genetic and nongenetic rodents in a cost-effective manner. Examples of tests offered include hyperinsulinemic-euglycemic clamp in conscious mice, a gold standard for glucose homeostasis and insulin sensitivity / resistance at the whole body and organ (liver, adipose and skeletal muscle) level, oxygen consumption rate and extra cellular acidification rate measurements from cells in culture plates, NMR-based magnetic resonance imaging system for body composition; simultaneous measurements of food intake, energy expenditure, heat and real time body temperature; home cage activity monitoring system. MMC directors provide consultation on selecting the appropriate tests and procedures, and the interpretation of data. Go to the Mouse Metabolism Core.
Director: Lawrence Chan, MD
Co-Director: Pradip Saha, Ph.D.
The Mouse Phenotyping Core facility contains a multitude of testing capabilities for the assessment of mouse models from embryo to adult. Access to the MPC is open to all Baylor and non-affiliated Baylor investigators. Current equipment includes: MRI, CT, ultrasound, body composition/densitometry, indirect calorimetry, telemetry, and indirect blood pressure. Workstations for image reconstruction and data analysis are also available within the core. Go to the Mouse Phenotyping Core website.
Lab Manager: Corey Reynolds, Ph.D.
Phone: (713) 798-5040
Optical Imaging and Vital Microscopy
The Optical Imaging and Vital Microscopy core offers equipment, training and support for vital and intravital imaging studies in cells, tissues, embryos and live mice. Available technologies include confocal microscopy, line scanning confocal microscopy, multispectral microscopy and two-photon microscopy using microscopes designed for live cell and live animal imaging. Go to the Optical Imaging and Vital Microscopy website.
Core Director: Dr. Mary Dickinson
Lab Manager: Dr. Tegy Vadakkan
Pathway Discovery Proteomics
This is a unique proteomic service that provides antibody affinity purification of endogenous protein complexes and MS-based identification and analysis of the associated proteins. Suitable antibodies for immunoprecipitation of the desired protein complex and optimization of the cell line, culture conditions, and methods for cell lysis and antibody affinity purification are provided as services. The complexes are isolated under conditions of cellular activation by appropriate signals as requested by the cancer center investigator. Go to the Pathway Discovery Proteomics website.
Director: Jun Qin, Ph.D.
Population Biosciences Biorepository
The Dan L. Duncan Cancer Center Population Sciences Biorepository was created to support a high-quality molecular epidemiology program in the Dan L. Duncan Cancer Center and to serve as a scientific resource for investigators on biospecimen collection and storage methods in their efforts to discover etiologic risk factors and deliver personalized cancer medicine. Our goal is to provide necessary risk factor and clinical data collection and a centralized, cost-effective facility for biospecimen processing and storage of biospecimens from large epidemiological and clinical studies. The PSB provides services for individually funded investigators as well as for clinical centers wishing to bank specimens prospectively from their patients for future research projects. The PSB clinical coordinators can assist you with consenting, phlebotomy, and data collection, if needed. The PSB Laboratory provides full fractionation and aliquoting for blood samples, as well as DNA extraction from whole blood, buffy coat, or saliva. Our team can consult with you to plan the data and specimen collection and storage needs for your projects. Go to the Population Biosciences Biorepository.
Director: Michael Scheurer, Ph.D.
RNA In Situ Hybridization
The RNA In Situ Hybridization core provides services and equipment to determine gene expression patterns in rodent and human tissues. We can process and section tissue (frozen or paraffin), prepare labeled RNA probes, perform high-throughput in situ hybridization, image and quantify the gene expression levels. Go to the RNA In Situ Hybridization website.
Director: Cecilia Ljungberg, Ph.D.
Phone: (832) 824-8873