The Antibody-Based Proteomics Core provides highly customized services for targeted proteomics platforms that are valuable tools for both validation and protein biomarker discovery, particularly for low abundance regulatory proteins and activation states of proteins with phosphorylated antibodies. Services provided include reverse phase protein arrays (RPPA) with over 240 antibodies for total and phosphoproteins of major cell signaling pathways, and Luminex bead technology for multiplex quantitative analyses of intracellular and extracellular signaling proteins. For more information, visit the Antibody-Based Proteomics website.
Core Director: Shixia Huang, Ph.D.
Phone: (713) 798-8722
The Bioengineering Core provides scientific instrumentation resources that catalyze the success of investigators by providing them with tools needed to design elegant experiments and ask truly cutting-edge research questions. Investigators from within and outside of Baylor College of Medicine are welcome. Services include customized scientific instrumentation design and manufacture, including electronics and optics. For more information, please visit the Bioengineering Core website.
Academic Director: Fabrizio Gabbiani, Ph.D.
Core Director: I-Chih Tan, Ph.D.
Phone: (713) 798-9168
Biostatistics and Informatics Shared Resource
The Biostatistics and Informatics Shared Resource (BISR) provides investigators with state-of-the-art biostatistical, bioinformatics, and high-performance computational infrastructure support for clinical, translational and basic science research. Major services include biostatistics and analytics, experimental design, assistance with design and conduct of clinical trials, and multi-omics data analysis. For more information, please visit the BISR website.
Academic Director: Susan Hilsenbeck, Ph.D.
Co-Director for Multi-Omics: Bioinformatics: Cristian Coarfa, Ph.D.
Co-Director for Cancer Bioinformatics: Chad Creighton, Ph.D.
Co-Director for General Biostatistics: Charles Minard, Ph.D.
Co-Director for Cancer Biostatistics: Tao Wang, Ph.D.
CAMRI-Core for Advanced MRI Imaging
The Core for Advanced MRI (CAMRI) provides technology and services for advanced imaging studies of the function, physiology and anatomy of humans and large animals, with a special emphasis on blood-oxygen level dependent functional magnetic resonance imaging (BOLD fMRI) of the human brain. The center houses two Siemens MRI Scanners. A Magnetom Trio and a PrismaFit (3 tesla field strength). A full complement of product pulse sequences are available for high-resolution structural imaging, diffusion tensor imaging (DTI), fMRI, Arterial Spin Labeling (ASL) and single and multi-voxel magnetic resonance spectroscopy (MRS). Research sequences are available for specialized imaging applications. For functional studies, equipment is available for stimulation of the auditory, visual, somatosensory and gustatory modalities, for infrared eye tracking of subject eye position, and for manual and vocal response recording. Consultation with a physicist is available for pulse sequence optimization and design. Users can scan with a MR technologist, or can elect to obtain training to scan independently. Go to the Core for Advanced MRI (CAMRI) page for more information.
Academic Director: Michael Beauchamp, Ph.D.
Technical Core Director: Meghan Robinson, Ph.D.
Operations Director: Krista Runge, M.S., R.T. (R)(MR)
Cell Based Assay Screening Service
The Cell-Based Assay Screening Services (C-BASS) Core is built upon the interconnected and complementary technology platforms of RNAi-based functional genomics and CRISPR/Cas9-mediated genome editing. The shared resource houses whole-genome RNAi libraries that enable expedient investigation of gene function either individually or in large scale. For genome editing, we have available a collection of CRISPR sgRNA and Cas9 vectors for distribution and cloning services, and assist investigators in deciding on the choice of cell line(s), optimal vectors, and types of Cas9. C-BASS is able to customize CRISPR/Cas9-mediated gene knockout (KO) and knock-in (KI) services, from target site design to generating KO/KI cell lines, based on the specific needs of individual investigators. For more information, visit the C-BASS website.
Core Director: Dan Liu, Ph.D.
Phone: (713) 798-8032
The CryoEM Core provides state of the art services for imaging and 3-D reconstruction of individual molecules, macromolecules and, in limited situations, macromolecular structure within cells. This technique can provide structures at resolutions comparable to X-ray crystallography without the need to grow crystals, and with very small quantities of material. We are equipped with three Cryo TEMs all with current generation direct detector technology, and our services range from specimen screening to high resolution data collection and reconstruction. For more details, please see the CryoEM Core Website.
Core Director: Steve Ludtke, Ph.D.
Co-Director: Zhao Wang, M.D., Ph.D.
Phone: (713) 798-6989
Cytometry and Cell Sorting
The Cytometry and Cell Sorting Core is a state-of-the-art facility offering assisted and unassisted flow cytometric services. Instrumentation includes FACSAria cell sorters with up to 13 colors plus forward and side scatter, LSRII Analyzers with high through-put 96 well sample loading plates, VI-Cell counter, AutoMACS magnetic bead cell sorter and workstations with data analysis software. High-parameter cytometry is also available including the Fluidigm Helios Mass Cytometer with Hyperion imaging system (100+ potential channels) and a 30+ parameter BD Symphony A5 flow cytometer. The shared resource provides training on all instruments, assists with data analysis and design of experiments, and is available 24 hours-a-day, 7 days-a-week for trained users. For more information. For more information, visit the Cytometry and Cell Sorting Core website.
Academic Director: Christine Beeton, Ph.D.
Core Director: Joel Sederstrom, M.B.S.
Phone: (713) 798-3868
The Gene Vector Core provides viral vector packaging and cloning/plasmid preparation service. Of the available gene delivery systems, virus-derived vectors are among the most efficacious, as they retain the native features tested in evolutionary pressure. We offer production/purification of popular viral vectors including adenovirus, adeno-associated virus, lentivirus and retrovirus at very competitive price. We work with investigators by incorporating cutting edge technologies while ensuring the high quality control. For more information, visit the Gene Vector website.
Core Director: Kazuhiro Oka, Ph.D.
Phone: (713) 798-7381
Genetically Engineered Mouse
The Genetically Engineered Mouse (GEM) Core provides specialized expertise and equipment for producing genetically modified mice essential for investigators working with mouse experimental models. The Core performs microinjections of mouse ES cells, transgene DNA constructs, and CRISPR sgRNA/Cas9/donor DNA into mouse embryos to generate genetically engineered founder mice. Also provided are services for cryopreservation of mouse embryos and sperm for long-term storage of mouse lines, mouse line re-derivation to remove pathogen contamination, re-establishment and expansion of mouse colonies, help with importation of mouse lines into BCM facilities and in vitro fertilization. Although the GEM Core will accept ES cells and CRISPR sgRNA/Cas9/donor DNA samples directly from investigators for microinjection, the preferred process is to use genetic manipulated ES cells and CRISPR sgRNA/Cas9 samples prepared as services by the Mouse ES Cell Core. The GEM Core and the Mouse ES Cell Core work collaboratively to coordinate these services. For more information, visit the Genetically Engineered Mouse website.
Academic Director: Jianming Xu, Ph.D.
Core Director: Lan Liao, M.S.
Phone: (713) 798-5278
Genomic and RNA Profiling (GARP)
The Genomic and RNA Profiling Core (GARP) provides researchers access to state-of-the-art genomics profiling technology and services including Next-Generation Sequencing on Illumina platforms (iSeq 100, NextSeq 500 and NovaSeq 6000) and the Nanostring nCounter technology for targeted gene interrogation. Our library prep automation system (SMARTer Apollo) enables us to process low- and high-throughput projects with minimal batch effects. Also available are automated DNA size selection on the Pippin HT and nucleic acid shearing on the Covaris LE220. GARP serves as a source for sample quality control, experimental design and NGS expertise. For more information, visit the GARP website.
Academic Director: Lisa White, Ph.D.
Phone: (713) 798-7699
Technical Core Director: Daniel Kraushaar, Ph.D.
Phone: (713) 798-7787
Human Stem Cell
The Human Stem Cell Core (HSCC) Core provides a wide range of products and services related to human pluripotent stem cell (hPSC) research, as well as hands-on training classes for basic and advanced stem cell culture techniques. We offer cost-effective solutions to generate and characterize new induced pluripotent stem (iPS) cells for in vitro disease modeling, employing non-integrating vector technologies such as Sendai virus-based and episomal vector-based reprogramming. We also offer customized research support for experimental design and validation assays, as well as genome editing of hPSCs using CRISPR/Cas9. For more information, please visit the Human Stem Cell Core website.
Core Director: Jean J. Kim, Ph.D.
Phone: (713) 798-7471
Human Tissue Acquisition and Pathology
The Human Tissue Acquisition and Pathology (HTAP) Core provides tissue and serum related services to researchers that includes histology and immunohistochemistry (IHC) and organization of the different tissue banks at BCM. Histology and IHC of human and experimental animal tissues are available on a fee-for-service basis. Services include tissue processing, embedding, sectioning and staining for routine histology (H&E, PAS, ORO, Trichrome, VVG, Giemsa), IHC and TUNEL assay for apoptosis. Also available are laser capture microdissection, archival tissue microarrays (TMA), image analysis by Inform and Vectra and consultation with pathologists. For more information, please visit the Human Tissue Acquisition and Pathology website.
Academic Director: Michael Ittmann, M.D., Ph.D.
Core Director: Patricia Castro, Ph.D.
Phone: (713) 798-6196
The Integrated Microscopy Core (IMC is designed to provide high quality microscopy and analytics services through availability of state-of-the-art imaging instrumentation, custom image analysis, one-on-one training by expert staff, plus free consultation for experimental setup, data acquisition and analysis. IMC covers most of the investigators’ needs in the areas of light microscopy with particular emphasis on super-resolution techniques, high throughput/high content phenotypic screening (assay development/validation), live imaging, multi-dimensional spatial analysis in tissue and cell culture samples, 3D culture models imaging and custom image analysis pipelines & data analytics. For more information, visit the Integrated Microscopy website.
Academic Director: Michael Mancini, Ph.D.
Core Director: Fabio Stossi, Ph.D.
Phone: (713) 798-6940
Macromolecular X-Ray Crystallography
The Macromolecular X-ray Crystallography Core provides a centralized and cost-efficient solution to determine the atomic structures of biologically important macromolecules. Unlike other structure analysis techniques, X-ray crystallography is not limited by the size or complex nature of the macromolecule of interest. One of the major advantages of this technique is that atomic resolution structural information is obtained routinely, revealing intimate details regarding the stereochemistry of the molecular interactions underlying the biological function of the macromolecule of interest. For more information, please visit the Macromolecular X-Ray Crystallography Core website.
Academic Director: Francis T.F. Tsai, Ph.D.
Core Director: Sukyeong Lee, Ph.D.
Mass Spectrometry Proteomics
The Mass Spectrometry Proteomics Core features state-of-the-art Thermo Scientific Orbitrap instruments (Lumos ETD Tribrid, Q-Exactive Plus, Fusion Tribrid, Explorer 480) and offers comprehensive service packages for proteome-wide label free quantitative profiling of cells and tissues, isolation and identification of protein complexes via immunoprecipitation and other affinity methods followed by mass spectrometry, post-translational modification analysis, and routine protein identification from purified samples. Full support is provided that includes project evaluation and design, biochemical purifications, mass spectrometry sequencing, and data analysis. Go to the Mass Spectrometry Proteomics website.
Academic Director: Anna Malovannaya, Ph.D.
Core Directors: Sung Yun Jung, Ph.D.
The Metabolomics Shared Resource provides various metabolomic procedures for discovery and validation of small molecular weight compound biomarkers of various diseases with state-of-the-art high mass spectrometry as the main technology platform. Services include quantitative targeted steady-state analysis of metabolites (up to 500 metabolites) for a wide range of analyte classes derived from biofluids, cell lines or tissues. Services also include measurement of flux in cell lines and in mice through various metabolic pathways by 13C-isotopomer methods, structural identification of unknown molecules and unbiased lipidomics profiling. For more information, visit the Metabolomics Core website.
Academic Director: Nagireddy Putluri, Ph.D.
Phone: (713) 798-3139
The tetramer production core provides customized MHC/peptide tetramers for identification of antigen-specific T lymphocytes by flow cytometry. Tetramers and/or their derivatives are provided for research purposes only; their use for commercial purposes is prohibited. Go to the MHC Tetramer website.
Director: Xiaochi Lily Wang, M.S.
Phone: (713) 798-3918
Mouse Embryonic Stem Cell
The Mouse ES Cell Core assists investigators with projects that involve generating mutations in the germline and somatic tissues of mice by performing homologous recombination in ES cells. Mouse ES cell services include electroporation, Rosa26 and Hprt locus targeting, clone expansion, the generation of primary mouse ES cell lines, and murine virus testing. In addition, the core provides specialized expertise essential for the production of genetically engineered mice using CRISPR/Cas9 genome editing. Core CRISPR/Cas9 consultation services include: sgRNA target site selection, selection of cytoplasmic or pronuclear injections of Cas9 mRNA or protein, and design of plasmid or oligo donor DNAs for homology directed repair. Core CRISPR/Cas9 production services include: sgRNA DNA template production, in vitro transcription of sgRNAs and Cas9, and design and implementation of founder genotyping. The Mouse ES Cell Core works closely with the Genetically Engineered Mouse (GEM) Core, which provides embryo microinjection services for our genetically manipulated ES cells and CRISPR reagents. For more information, please visit the Mouse Embryonic Stem Cell Core website.
Academic Director: Jason D. Heaney, Ph.D.
Core Director: Isabel Lorenzo, B.S.
CRISPR Technologies Manager: Denise G. Lanza, Ph.D.
Phone: (713) 798-1981; (713) 798-3115
Mouse Metabolism and Phenotyping
The Mouse Metabolism and Phenotyping Core (MMPC) is a comprehensive fee-for-service rodent phenotyping core that contains a multitude of testing capabilities for the assessment of rodent models from embryo to adult. The MMPC provides a variety of state-of-the-art equipment and techniques with the goal to standardize key methodologies and to expedite comprehensive research analyses on diseases related to cancer, cardiovascular dysfunction, metabolic disorders, mouse models of human disease, and drug studies. The MMPC also supports the application of advanced analysis of metabolic pathways and related physiological and biochemical parameters in mice and rats, as well as in isolated / cultured cells in vitro. Metabolic assays offered include hyperinsulinemic-euglycemic clamp in conscious mice, glucose metabolism, lipid metabolism, oxygen consumption rate and extra cellular acidification rate measurements from cells in culture plates, DEXA and Echo MRI, CLAMS, real time body temperature monitoring, home cage activity monitoring, MRI, CT, ultrasound, Xray, bioluminescence imaging, indirect calorimetry, telemetry, and non-invasive blood pressure measurements. Workstations for image reconstruction and data analysis are also available. Core personnel provide consultation on selecting the appropriate tests and procedures, and the interpretation of data. For more information, please visit the Mouse Metabolic and Phenotyping Core website.
Core Director: Christopher Ward, Ph.D.
Co-Director: Pradip Saha, Ph.D.
NMR & Drug Metabolism Core
The NMR and Drug Metabolism Core offers tools to support the discovery, synthesis, screening, optimization, metabolism and pharmacokinetics of small molecule ligands or lead compounds. Our 600 and 800 MHz Bruker nuclear magnetic resonance (NMR) spectrometers are available for unassisted use by trained users, and expert services are available to determine small molecule identity or conformation, to identify and quantify metabolites, to determine macromolecular structure or detect perturbations upon ligand binding, or to screen compound libraries against purified targets. DNA-encoded technology available through the Center for Drug Discovery allows affinity-based selection of millions of different molecules in a single tube to identify those that most tightly bind a tagged, purified protein. We investigate the metabolism, metabolic stability, and pharmacokinetics of small molecular weight compounds using liquid chromatography-mass spectrometry (LC-MS and MS/MS). In addition to analyzing biological fluids or tissues, we assay stability in liver microsomes and perform reaction phenotyping against an array of CYP450s. Core personnel provide advice on the use of the supported methods for a wide variety of drug metabolism and pharmacokinetics (DMPK) applications and assistance is available in project experimental design and data analysis. For more information, please go to the NMR and Drug Metabolism website.
Academic Director: Kevin MacKenzie, Ph.D.
Phone: (832) 824-8155
Co-Director: Feng Li, Ph.D.
Phone: (713) 798-3623
Operations Director: Kim Kerlec
Phone: (713) 798-8132
Optical Imaging & Vital Microscopy (OiVM)
The Optical Imaging & Vital Microscopy (OiVM) core is a full service microscopy lab equipped with cutting edge 3D imaging tools including the latest generation confocal, 2-photon and super-resolution microscopes; Lightsheet, Optical Projection Tomography (OPT) and XRay µCT instruments as well as high speed, multi-spectral fluorescence imaging systems. OiVM instruments are designed for vital and intravital imaging of processes within cells, intact tissue explants, developing embryos and functioning organs within the live animal. In addition to cutting edge imaging systems, the core provides tissue clearing services utilizing CLARITY for intact organ and embryo imaging and advanced 3D rendering environments for data visualization and analysis. The OiVM core is open to all TMC institutions and offers assisted vs. unassisted use rates, bulk pricing, 24/7 independent user access, and a staff of experienced microscopists for consultation, experiment design, training and instruction on a wide variety of microscopy techniques. For more information, please visit the Optical Imaging and Vital Microscopy website.
Academic Director: Mary E. Dickinson, Ph.D.
Core Director: Jason M. Kirk
Phone: (713) 798-6486 (OIVM)
Patient-Derived Xenograft and Advanced In Vivo Models
The Patient-Derived Xenograft and Advanced In Vivo Models (PDX-AIM) Core of Baylor College of Medicine is divided into two independent but closely interacting functional units. The PDX Development Unit was created to facilitate the establishment and use of PDXs using immunocompromised mice as the host species, with an emphasis on development of databases and computational tools to enhance PDX collection management and data display. The Advanced In Vivo Models Unit was created to facilitate establishment and use of patient material grown on the chorioallantoic membrane (CAM) of the chicken egg. The Advanced In Vivo Models Unit also employs the CAM model to establish non-tumorigenic organoids, 3-D tumors from existing cancer cell lines, and creation and optimization of custom bioassays to assess tumor neovascularization, invasion, and metastasis. For more information, please visit the Patient-Derived Xenograft and Advanced In Vivo Models Core site.
PDX Development Unit:
Academic Director: Michael Lewis, Ph.D.
Core Director: Lacey Dobrolecki, MT, MS
Advanced In Vivo CAM Models Unit:
Academic Director: Andrew Sikora, M.D., Ph.D.
Core Director: Hugo Villanueva, Ph.D.
Population Sciences Biorepository
The Population Sciences Biorepository Shared Resource provides risk factor and clinical data collection and a centralized facility for biospecimen processing and storage from epidemiological and clinical studies. Services are available for individual investigators as well as for clinical centers that require prospective banking of patient specimens. Clinical coordinators can assist with consenting, phlebotomy, and data collection. The PSB also provides laboratory services including: full fractionation and aliquoting for blood samples; DNA extraction from whole blood, buffy coat, or saliva; and RNA extraction from whole blood. For more information, please visit the Population Sciences Biorepository.
Director: Michael Scheurer, Ph.D.
Co-Director: Marilsa Hardy DrPH, M.P.H.
Protein and Monoclonal Antibody Production
The Protein and Monoclonal Antibody Production Core provides investigators with high quality mouse monoclonal antibodies (MAbs) and purified recombinant proteins to facilitate their research programs. The Core has experience with intact proteins, synthetic peptides, and subcellular fractions as antigens in generating MAbs that perform for a wide range of applications including, but not limited to immunoblotting, immunoprecipitation, ELISA, immunocytochemistry, and IHC. Expression and purification of recombinant proteins of interest is a parallel service that involves consultation on design and construction of expression vectors, protein overexpression in the baculovirus insect cell system, E. coli or mammalian cells, and purification of the expressed protein. Characterization of protein products for purity and other quality controls is provided for suitability of proteins in downstream biochemical and other applications. For more information, please visit the Protein and Monoclonal Antibody Production website.
Senior Faculty Advisor: Dean P. Edwards, Ph.D.
Core Director: Yingmin Zhu, Ph.D.
Lab Director: Kurt Christensen
Phone: (713) 798-2325 & (713)-798-5012
RNA-In Situ Hybridization
The RNA-In Situ Hybridization core provides services and equipment to determine gene expression patterns in rodent and human tissues. We can process and section tissue (frozen or paraffin), prepare labeled RNA probes, perform high-throughput in situ hybridization, image and quantify the gene expression levels. For more information, please visit the RNA In Situ Hybridization website.
Director: Cecilia Ljungberg, Ph.D.
Phone: (832) 824-8873
Single Cell Genomics
The Single Cell Genomics Core (SCGC) provides comprehensive services for RNA and chromatin profiling on single or a small number of cells. The core is equipped with the 10x Genomics Chromium Platform, the Takara icell8 platform, and the Fluidigm C1 auto cell prep platforms. The combination of these platforms allows cost-effective and high throughput genomics profiling that matches with the experiments. Next-gen sequencing of libraries prepared by the SCGC is done as a collaboration with the Genomics and RNA Profiling Core. For more information, please visit the Single Cell Genomics Core website.
Academic Director: Rui Chen, Ph.D
Phone: (713) 798-5194
Technical Core Director: Yumei Li, Ph.D.
Phone: (713) 798-7565
Small Animal MRI
A state-of-the-art Bruker BioSpec® 9.4T horizontal bore MRI scanner is equipped to perform a wide variety of magnetic resonance imaging and spectroscopy studies on small animals (mice and rats) for non-invasive, high-resolution longitudinal imaging for translational research. Go to Small Animal MRI.
Director: Robia G. Pautler, Ph.D.
Phone: (713) 798-3892