Metabolomics is an emerging and potentially potent tool in healthcare and biomarker discovery to understand the disease states and mechanistic oversight of the disease. Though biological processes operate through complex interactions between genes, RNA, proteins, and metabolites, the composite of this complex interaction network is described as the interactome. Metabolomics is a complementary tool to understand the interactome in disease progression including cancers, which studies endogenous and exogenous metabolites in biological systems.
Metabolomics core has the capability to quantify relative abundances of more than 800 metabolites of different pathway-specific metabolites such as amino sugars, amino acids, prostaglandins, carnitines, polyamines, glycolysis intermediates, TCA cycle, nucleotides, vitamins, steroids, bile acids, short-chain fatty acids, methylated metabolites, fatty acids, Neurotransmitters and related metabolites using mass spectrometry.
All analysis steps, including sample extraction, running the LC-MS, data analysis, and data interpretation, are conducted by well-trained mass spectrometry scientists. The core directors and staff ensure quality control during metabolites extraction using highly curated spiked internal and matrix pools. Afterward, the core director monitors and supervises the entire procedure, from sample extraction to data analysis. Core Standard operation Procedure (SOP), we have been using quality control (QC) along with biological experimental samples to check the extraction efficiency /instrument variations.
Liquid chromatography (Agilent 1290 Infinity II) separates the compounds from biological matrixes and is then identified by mass spectrometry (Agilent QQQ 6495 and 6495B). In addition, the metabolite compounds are often analyzed in Single Reaction Monitoring (SRM) mode. All assays performed in the metabolomics core are standardized with proper validation in different matrices.
If the investigator is interested in performing other assays not listed on the webpage, either relative or absolute quantification, please get in touch with Core Directors.