About the Core
Antibody-based Proteomics (ABP) Core offers Reverse Phase Protein Array (RPPA) and complementary antibody-based proteomics platforms, including Olink Reveal, Luminex multiplex quantitative assays, and the automated Ella immunoassay system. Among these, Olink Reveal is a cost-effective, NGS-based technology for rapid, high-throughput protein discovery, enabling the measurement of more than 1,000 protein targets across diverse research areas such as inflammation, signal transduction, metabolism, and development.
RPPA is a high-throughput proteomics platform that enables simultaneous quantification of protein expression and post-translational modifications, such as phosphorylation, across thousands of samples, including tissue and cell lysates. The RPPA Core maintains an inventory of more than 360 rigorously validated antibodies covering key biological pathways and functional groups, including the tumor microenvironment, epithelial–mesenchymal transition, stemness, apoptosis, DNA damage, autophagy, proliferation and cell cycle, inflammation, and chromatin regulatory proteins. In addition, a dedicated epigenetic RPPA platform is available for profiling histone marks. Working closely with investigators, the Core continuously expands assay coverage, supports both full-panel and customized RPPA panels, and provides in-house data normalization and statistical analysis.
Getting Started
If you plan to make use of the Antibody-based Proteomics Core, we require a brief, no-cost consultation with Dr. Huang or Dr. Shi for new projects. Please download and fill out the Service Request Project Info Form and email it to us first. Once the discussion about the project design and the timeline is complete, an iLabs project request is required.
If you are an experienced Antibody-based Proteomics Core user, you will still need to fill out the project information form, but we will waive the meeting requirement. CPRIT-subsidized RPPA projects require a meeting and additional information. Contact us for details.