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Research

NanoString

Master
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NanoString technology is a direct digital detection system, which enables targeted multiplex analysis of RNA and DNA that does not require amplification or enzymology. It can simultaneously analyze from 20-800 genes or gene loci from small samples including tissue, blood, FFPE and single cells. Sample types include total RNA, mRNA, miRNA, lncRNAs, RNA prepared by RIP and DNA enriched from chromatin immunoprecipitation (ChIP) as an alternative to ChIP-Seq.

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NanoString Input Amounts

Sample Input Type

Input Amount

Minimum Concentration

Total RNA

100 ng

33.3 ng/μl

Cell Lysate

10,000 cells in 30 μl

 

ChIP DNA

10ng unamplified ChIP DNA

100ng of WGA amplified ChIP DNA or NGS library prepared DNA

3.33 ng/ul

33.3 ng/μl

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NanoString offers off-the-shelf products as well as custom sets. All off-the-shelf products are available in sets of 12, 24, 48, 96, 192, 384, 576, 768 or 960 assays. Visit NanoString's website for an up-to-date list of available CodeSets.

For more information about miRNA profiling from blood plasma and serum, please see NanoString's miRNA Analysis in Plasma and Serum Samples Tech Note and Recommendations for miRNA Purification from Blood Plasma and Serum.

For more information about NanoString and the nCounter, please visit the NanoString website. Read NanoString Tech Note here.

For pricing, please email GARP at garpcore@bcm.edu.

See the NanoString Workflow.