NGS Platforms

Illumina’s iSeq, NextSeq and NovaSeq systems' powerful combination of read-length and paired-end flexibility enables the broadest range of genomic sequencing applications. Illumina's robust sequencing chemistry supports a wide range of read lengths, allowing researchers to tailor each run to meet their needs with shorter turnover times.

Illumina Sequencing Instrument Specifications
Instrument	iSeq 100 Sequencer	NextSeq 500 Sequencer	NextSeq 500 Sequencer	NovaSeq 6000 Sequencer	NovaSeq 6000 Sequencer	NovaSeq 6000 Sequencer	NovaSeq 6000 Sequencer
Mode	Regular	Mid Output	High Output	SP FC	S1 FC	S2 FC	S4 FC
Output (Max.)	1.2 Gb - 2x150bp	32.5-39 Gb - 2x150bp 16.25-19.5 Gb - 2x75bp	100-120 Gb - 2x150bp 50-60 Gb - 2x75bp 25-30 Gb - 1x75bp	400 Gb - single FC	500 Gb - single FC	1250 Gb - single FC	3000 Gb - single FC
Run Time	17.5 hours	15 - 26 hours	11 - 29 hours	13 - 38 hours	13 - 25 hours	16 - 36 hours	45 hours
Single End Reads (Max.)	Up to 4 Million	Up to 130 Million	Up to 400 Million	800 Million	1.6 Billion	4.1 Billion	10 Billion
Paired End Reads (Max.)	Up to 8 Million	Up to 260 Million	Up to 800 Million	1.6 Billion	3.2 Billion	8.2 Billion	20 Billion
Read Length (Max.)	2 x 150 bp	2 x 150 bp	2 x 150 bp	2 x 250 bp	2 x 150 bp	2 x 150 bp	2 x 150 bp
Bases above Q30	> 80%	>75% (2 x 150 bp) >80% (2 x 75 bp)	>75% (2 x 150 bp) >80% (2 x 75 bp) >80% (1 x 75 bp)	≥85% (2 x 50 bp) ≥75% (2 x 150 bp) ≥75% (2 x 250 bp)	≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp)	≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp)	≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp)

Gb = gigabases, bp = base pair

1 Flow Cell (FC) = 8 Lanes

For more information about these instruments see: