Integrated Microscopy Core


GE Healthcare DeltaVision OMX 3D-SIM

The DeltaVision OMX is an advanced, multimode super-resolution microscope system offering the Blaze SIM Module for dynamic, high speed 3D-SIM.

The DeltaVision OMX is a 3D structured illumination microscope (3D-SIM) designed to provide a highly stable, multi-channel imaging platform for advanced microscopy applications. The design of the microscope is such that super-resolution 3D images may be rapidly obtained and constructed. Live or fixed cells may be imaged.

Key features include:
• Multiple illumination modalities
      · Structured illumination 3D SIM
      · Advanced TIRF and pK/pA module
      · Localization imaging
      · Wide-field imaging
• SIM Blaze module for full 3D super-resolution reconstruction
• UltimateFocus laser-based hardware autofocus technology
• "Pan and scan" function
• High precision motor-driven stage

Cameras: PCO front illuminated Edge sCMOS x 2. 15-bit, 5.5Mp, 512x512 for 3D-SIM; 1024x1024 for wide-field and TIRF. High speed, low light sensitivity.

Acquisition software: OMX Master Control Workstation (Windows 7)

Workstation: softWoRx®/Imaris (CentOS v6, Linux)

Filter sets: 2x 8 position filter wheels

Lasers: 405nm ± 2; 488nm ± 2; 568nm ± 1; 642nm ± 2. 4-channel simultaneous imaging capability.

• 60X oil PLANPON6, NA 1.42 (required for use with sCMOS camera)
• 60X APON, NA 1.49 with correction collar (required for TIRF)

Illumination: 6-color InsightSSI solid state wide-field illumination. Supports DAPI/CFP/GFP, FITC/YFP/Alexa Fluor™ 568, mCherry/Cy™5/DIC. 

Nikon A1R-s Confocal Microscope

The A1R-s 4-laser confocal microscope can be used to capture projection images, FRAP images, FRET images or Z-stacks from thicker sections or tissues. The live chamber is balanced for humidity, temperature and CO2.

The Nikon A1Rs confocal laser scanning microscope has both high resolution (4Kx4K) and high speed (up to 230 frames/sec) imaging capabilities. The system can perform real time FRAP, calcium imaging (with appropriate dyes), FRET, spectral detection (with adequate control samples) and time lapse studies of live cells.

Key features include:
 4 PMTs plus white light detector
• 7 Laser Lines:
 - 405nm diode at 38mW
 - Argon multi-line laser:457.9nm/476.5nm/488nm/514.5nm
 - 561.7nm diode at 40mW
 - 637.9nm diode at 10mW
• Spectral detector
• Nikon Perfect Focus System (for live imaging)
• Regulated environmental chamber for live cells
 - CO2, temperature, humidity

Acquisition Software: Nikon Elements

Filter sets: Standard DAPI/FITC/TxRED/Cy5

Objective lenses:
 10x Plan Fluor/0.3 NA w/phase
 20x Plan Apo/0.75 NA
• 40x Plan Fluor/0.75 NA
• 40x Plan Fluor/1.3 NA Oil
• 60x Plan Apo/1.4 NA Oil
• 100x Plan Apo/1.4 Oil

Illumination: X-Cite 120 long life fluorescent lamp, trans-illumination, DIC

For best results use 1.5 oz. (#1.5, 170 micron) cover slips.

GE Healthcare DeltaVision LIVE High Resolution Deconvolution (Image Restoration) Microscope

The deconvolution/restoration microscope is an excellent choice for fast acquisition of fixed or live cells at low light levels.

The GE Healthcare Deconvolution (Restoration) Microscope is engineered to provide high contrast and high resolution at low light levels. The environmental chamber provides a temperature controlled, humidified environment with defined CO2 levels. Microscope capabilities include Z-sectioning and 3D reconstruction, time series capture for following cellular events, determining co-localization of multiple proteins and/or dyes and FRAP.

Key features include:
• High Resolution Photometrics CoolSNAP Camera
• High Sensitivity Photometrics HQ2 EMCCD Camera

Acquisition Software: SoftWoRx

Filter Sets:
• Live cell filter set - CFP/YFP/RFP or GFP/RF
• Standard filter set - DAPI/FITC/TRITC/CY5
• Alexa filter set - DAPI/FITC/Alexa594/Cy5
• Brightfield/DIC imaging capabilities

• 40X U Plan-FL N/0.75 NA, air gap
• 40X Plan-Apo/0.95 NA, air gap with correction collar
• 40X U Apo/1.35 NA, oil with iris
• 60X Plan-Apo/1.4 NA, oil
•100X U PlanS-Apo/1.4 NA, oil|

Illumination: 2 xenon lamps for multiplexing wavelengths

The base microscope is an Olympus IX-71 Accepts standard microscope slides, multiwell coverglass chambers, 35mm cell culture dishes and multiwell plates (up to 384 well format)

For best results use 1.5 oz (170 micron) coverslips.

Hitachi H7500 Transmission Electron Microscope

The Hitachi H7500 transmission electron microscope is suitable for the examination of biological samples and/or small particles.

The Hitachi H-7500 transmission electron microscope is an instrument suitable for high magnification examination of biological tissues in research. The column design of this particular TEM allows users to visualize samples at magnifications ranging from very low (700x) to very high (200,000x) while maintaining high resolution, a large field of view (160mm) and high contrast. The status of the electron microscope is displayed graphically on a dedicated computer, which shows the viewing and microscope parameters and allows the user to customize these conditions to suit their needs. An advanced vacuum system eliminates the need for a cold finger.

Key features include:
• Easy visualization of the TEM environment through
  graphic interface
• Point-to-point resolution of 0.4nm (ideal)
• 60, 80, or 100kV accelerating voltage
• Focusing wobbler and focus loupe tool
• High contrast viewing screen
• Easy to use, well marked controls
• Air table mounted to reduce vibration

Camera NEW!: AMT XR-16 16mp high resolution CCD camera

Acquisition Software: AMT Capture Engine, v602.600.51
                                            8- or 16-bit resolution capabilities

Illumination: long life tungsten hairpin filament

Nikon TE-2000

The Nikon TE-2000 is a manual inverted epifluorescence microscope with brightfield and DIC capabilities.

Camera: Cool Snap HQ B&W camera

Acquisition Software: Nikon Elements

Filter Sets: Standard DAPI/CFP/FITC/TxRED/Cy5

Objective Lenses:
• 10x Plan Fluor/0.30 NA
• 20x Plan Fluor/0.5 NA
• 20x S Plan Fluor ELWD/0.45 NA
• 40x S Plan Fluor ELWD/0.60 NA
• 40x Plan Apo/0.95 NA
• 60x Plan Apo VC/1.40 NA, oil or water; request
• 100x Plan Apo VC/1.40 NA, oil; request

Illumination: X-Cite 120 long life fluorescent lamp, trans-illumination, DIC

For best results use 1.5 oz. (#1.5, 170 micron) cover slips.