Research

Nikon A1R-s Confocal Microscope

Master
Content

The Nikon A1Rs confocal laser scanning microscope has both high resolution (4Kx4K) and high speed (up to 230 frames/sec) imaging capabilities. The system can perform real time FRAP, calcium imaging (with appropriate dyes), FRET, spectral detection (with adequate control samples) and time lapse studies of live cells.

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Key Features

Media Component
Nikon A1Rs
Content
  • 4 PMTs plus white light detector
  • 7 Laser Lines:
    • 405nm diode at 38mW
    • Argon multi-line laser:457.9nm/476.5nm/488nm/514.5nm
    • 561.7nm diode at 40mW
    • 637.9nm diode at 10mW
  • Spectral detector
  • Nikon Perfect Focus System (for live imaging)
  • Regulated environmental chamber for live cells - CO2, temperature, humidity

Acquisition Software

  • Nikon Elements

Filter Sets

  • Standard DAPI/FITC/TxRED/Cy5

Objective Lenses

  • 10x Plan Fluor/0.3 NA w/phase
  • 20x Plan Apo/0.75 NA
  • 40x Plan Fluor/0.75 NA
  • 40x Plan Fluor/1.3 NA Oil
  • • 60x Plan Apo/1.4 NA Oil
  • 100x Plan Apo/1.4 Oil

Illumination

  • X-Cite 120 long life fluorescent lamp, trans-illumination, DIC

For best results use 1.5 oz. (#1.5, 170 micron) cover slips.