The Nikon A1Rs confocal laser scanning microscope has both high resolution (4Kx4K) and high speed (up to 230 frames/sec) imaging capabilities. The system can perform real time FRAP, calcium imaging (with appropriate dyes), FRET, spectral detection (with adequate control samples) and time lapse studies of live cells.
Key features include:
• 4 PMTs plus white light detector
• 7 Laser Lines:
- 405nm diode at 38mW
- Argon multi-line laser:457.9nm/476.5nm/488nm/514.5nm
- 561.7nm diode at 40mW
- 637.9nm diode at 10mW
• Spectral detector
• Nikon Perfect Focus System (for live imaging)
• Regulated environmental chamber for live cells
- CO2, temperature, humidity
Acquisition Software: Nikon Elements
Filter sets: Standard DAPI/FITC/TxRED/Cy5
Objective lenses:
• 10x Plan Fluor/0.3 NA w/phase
• 20x Plan Apo/0.75 NA
• 40x Plan Fluor/0.75 NA
• 40x Plan Fluor/1.3 NA Oil
• 60x Plan Apo/1.4 NA Oil
• 100x Plan Apo/1.4 Oil
Illumination: X-Cite 120 long life fluorescent lamp, trans-illumination, DIC
For best results use 1.5 oz. (#1.5, 170 micron) cover slips.
Core Hours
Monday-Friday, 8:30 a.m. to 6 p.m.
Office phone: (713) 798-4679
Core Location
Cullen Building:
122A Light Microscopy
125A Electron Microscopy
117A, 119A, 121A (offices)
