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Research

Nikon A1R-s Confocal Microscope

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The Nikon A1Rs confocal laser scanning microscope has both high resolution (4Kx4K) and high speed (up to 230 frames/sec) imaging capabilities. The system can perform real time FRAP, calcium imaging (with appropriate dyes), FRET, spectral detection (with adequate control samples) and time lapse studies of live cells.

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Nikon A1Rs
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Key features include:
4 PMTs plus white light detector
• 7 Laser Lines:
- 405nm diode at 38mW
- Argon multi-line laser:457.9nm/476.5nm/488nm/514.5nm
- 561.7nm diode at 40mW
- 637.9nm diode at 10mW
• Spectral detector
• Nikon Perfect Focus System (for live imaging)
• Regulated environmental chamber for live cells
- CO2, temperature, humidity

Acquisition Software: Nikon Elements

Filter sets: Standard DAPI/FITC/TxRED/Cy5

Objective lenses:
10x Plan Fluor/0.3 NA w/phase
20x Plan Apo/0.75 NA
• 40x Plan Fluor/0.75 NA
• 40x Plan Fluor/1.3 NA Oil
• 60x Plan Apo/1.4 NA Oil
• 100x Plan Apo/1.4 Oil

Illumination: X-Cite 120 long life fluorescent lamp, trans-illumination, DIC

For best results use 1.5 oz. (#1.5, 170 micron) cover slips.

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Core Hours

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Monday-Friday, 8:30 a.m. to 6 p.m.

Office phone: (713) 798-4679

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Core Location

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Cullen Building:
122A Light Microscopy
125A Electron Microscopy
117A, 119A, 121A (offices)