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Research

GE Healthcare DeltaVision LIVE High Resolution Deconvolution Microscope

Master
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The GE Healthcare Deconvolution (Restoration) Microscope is engineered to provide high contrast and high resolution at low light levels. The environmental chamber provides a temperature controlled, humidified environment with defined CO2 levels. Microscope capabilities include Z-sectioning and 3D reconstruction, time series capture for following cellular events, determining co-localization of multiple proteins and/or dyes and FRAP.

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The deconvolution/restoration  microscope is an excellent choice for fast acquisition of fixed or live cells at low light levels.
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Key features include:
Cameras:
• High Resolution Photometrics CoolSNAP Camera
• High Sensitivity Photometrics HQ2 EMCCD Camera

Acquisition Software: SoftWoRx

Filter Sets:
• Live cell filter set - CFP/YFP/RFP or GFP/RF
• Standard filter set - DAPI/FITC/TRITC/CY5
• Alexa filter set - DAPI/FITC/Alexa594/Cy5
• Brightfield/DIC imaging capabilities

Objectives:
• 40X U Plan-FL N/0.75 NA, air gap
• 40X Plan-Apo/0.95 NA, air gap with correction collar
• 40X U Apo/1.35 NA, oil with iris
• 60X Plan-Apo/1.4 NA, oil
•100X U PlanS-Apo/1.4 NA, oil|

Illumination: 2 xenon lamps for multiplexing wavelengths

The base microscope is an Olympus IX-71 Accepts standard microscope slides, multiwell coverglass chambers, 35mm cell culture dishes and multiwell plates (up to 384 well format)

For best results use 1.5 oz (170 micron) coverslips.

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Core Hours

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Monday-Friday, 8:30 a.m. to 6 p.m.

Office phone: (713) 798-4679

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Core Location

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Cullen Building:
122A Light Microscopy
125A Electron Microscopy
117A, 119A, 121A (offices)