Research

GE Healthcare DeltaVision LIVE High Resolution Deconvolution Microscope

Master
Content

The GE Healthcare Deconvolution (Restoration) Microscope is engineered to provide high contrast and high resolution at low light levels. The environmental chamber provides a temperature controlled, humidified environment with defined CO2 levels. Microscope capabilities include Z-sectioning and 3D reconstruction, time series capture for following cellular events, determining co-localization of multiple proteins and/or dyes and FRAP.

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Key Features

Media Component
The deconvolution/restoration  microscope is an excellent choice for fast acquisition of fixed or live cells at low light levels.
Content

Cameras

  • High Resolution Photometrics CoolSNAP Camera
  • High Sensitivity Photometrics HQ2 EMCCD Camera

Acquisition Software

  • SoftWoRx

Filter Sets

  • Live cell filter set - CFP/YFP/RFP or GFP/RF
  • Standard filter set - DAPI/FITC/TRITC/CY5
  • Alexa filter set - DAPI/FITC/Alexa594/Cy5
  • Brightfield/DIC imaging capabilities

Objectives

  • 40X U Plan-FL N/0.75 NA, air gap
  • 40X Plan-Apo/0.95 NA, air gap with correction collar
  • 40X U Apo/1.35 NA, oil with iris
  • 60X Plan-Apo/1.4 NA, oil
  • •100X U PlanS-Apo/1.4 NA, oil|

Illumination

  • 2 xenon lamps for multiplexing wavelengths

The base microscope is an Olympus IX-71 Accepts standard microscope slides, multiwell coverglass chambers, 35mm cell culture dishes and multiwell plates (up to 384 well format)

For best results use 1.5 oz (170 micron) coverslips.