Illumina’s iSeq, NextSeq and NovaSeq systems' powerful combination of read-length and paired-end flexibility enables the broadest range of genomic sequencing applications. Illumina's robust sequencing chemistry supports a wide range of read lengths, allowing researchers to tailor each run to meet their needs with shorter turnover times.
|
Instrument |
iSeq 100 Sequencer |
NextSeq 500 Sequencer |
NextSeq 500 Sequencer |
NovaSeq 6000 Sequencer |
NovaSeq 6000 Sequencer |
NovaSeq 6000 Sequencer |
NovaSeq 6000 Sequencer |
|---|---|---|---|---|---|---|---|
|
Mode |
Regular |
Mid Output |
High Output |
SP FC |
S1 FC |
S2 FC |
S4 FC |
|
Output (Max.) |
1.2 Gb - 2x150bp |
32.5-39 Gb - 2x150bp 16.25-19.5 Gb - 2x75bp |
100-120 Gb - 2x150bp 50-60 Gb - 2x75bp 25-30 Gb - 1x75bp |
400 Gb - single FC |
500 Gb - single FC |
1250 Gb - single FC
|
3000 Gb - single FC |
|
Run Time |
17.5 hours |
15 - 26 hours |
11 - 29 hours |
13 - 38 hours |
13 - 25 hours |
16 - 36 hours |
45 hours |
|
Single End Reads (Max.) |
Up to 4 Million |
Up to 130 Million
|
Up to 400 Million
|
800 Million |
1.6 Billion |
4.1 Billion |
10 Billion |
|
Paired End Reads (Max.) |
Up to 8 Million |
Up to 260 Million |
Up to 800 Million |
1.6 Billion |
3.2 Billion |
8.2 Billion |
20 Billion |
|
Read Length (Max.) |
2 x 150 bp |
2 x 150 bp |
2 x 150 bp |
2 x 250 bp |
2 x 150 bp |
2 x 150 bp |
2 x 150 bp |
|
Bases above Q30 |
> 80% |
>75% (2 x 150 bp) >80% (2 x 75 bp)
|
>75% (2 x 150 bp) >80% (2 x 75 bp) >80% (1 x 75 bp) |
≥85% (2 x 50 bp) ≥75% (2 x 150 bp) ≥75% (2 x 250 bp) |
≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp) |
≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp) |
≥85% (2 x 50 bp) ≥80% (2 x 100 bp) ≥75% (2 x 150 bp) |
Gb = gigabases, bp = base pair
1 Flow Cell (FC) = 8 Lanes
For more information about these instruments see:
- iSeq 100 Sequencer
- NextSeq 500 Sequencer
- NovaSeq 6000 Sequencer
