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Research

NGS Platforms

Master
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Illumina’s iSeq, NextSeq and NovaSeq systems' powerful combination of read-length and paired-end flexibility enables the broadest range of genomic sequencing applications. Illumina's robust sequencing chemistry supports a wide range of read lengths, allowing researchers to tailor each run to meet their needs with shorter turnover times.

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Illumina Sequencing Instrument Specifications

Instrument

iSeq 100 Sequencer

NextSeq 500 Sequencer

NextSeq 500 Sequencer

NovaSeq 6000 Sequencer

NovaSeq 6000 Sequencer

NovaSeq 6000 Sequencer

NovaSeq 6000 Sequencer

Mode

Regular

Mid Output

High Output

SP FC

S1 FC

S2 FC

S4 FC

Output (Max.)

1.2 Gb - 2x150bp

32.5-39 Gb - 2x150bp

16.25-19.5 Gb - 2x75bp

100-120 Gb - 2x150bp

50-60 Gb - 2x75bp

25-30 Gb - 1x75bp

400 Gb - single FC

500 Gb - single FC

 

1250 Gb - single FC

 

3000 Gb - single FC

Run Time

17.5 hours

15 - 26 hours

11 - 29 hours

13 - 38 hours

13 - 25 hours

16 - 36 hours

45 hours

Single End Reads (Max.)

Up to 4 Million

Up to 130 Million

 

Up to 400 Million

 

800 Million

1.6 Billion

4.1 Billion

10 Billion

Paired End Reads (Max.)

Up to 8 Million

Up to 260 Million

Up to 800 Million

1.6 Billion

3.2 Billion

8.2 Billion

20 Billion

Read Length (Max.)

2 x 150 bp

2 x 150 bp

2 x 150 bp

2 x 250 bp

2 x 150 bp

2 x 150 bp

2 x 150 bp

Bases above Q30

> 80%

>75% (2 x 150 bp)

>80% (2 x 75 bp)

 

>75% (2 x 150 bp)

>80% (2 x 75 bp)

>80% (1 x 75 bp)

≥85% (2 x 50 bp)

≥75% (2 x 150 bp)

≥75% (2 x 250 bp)

≥85% (2 x 50 bp)

≥80% (2 x 100 bp)

≥75% (2 x 150 bp)

≥85% (2 x 50 bp)

≥80% (2 x 100 bp)

≥75% (2 x 150 bp)

≥85% (2 x 50 bp)

≥80% (2 x 100 bp)

≥75% (2 x 150 bp)

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Gb = gigabases, bp = base pair

1 Flow Cell (FC) = 8 Lanes

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For more information about these instruments see: