IDDRC In Vivo Neurophysiology Core

• Project consultation
• Stereotaxic implantation in mice 
• Video-EEG recordings in freely moving mice
• Deep brain stimulation in freely moving mice
• Local field recordings in freely moving mice
• Hippocampal LTP induction and recording in anesthetized mice
• Hippocampal LTP induction and recording in freely moving mice
• Mouse behavioral tests (elevated plus maze, open field assay, light/dark test, foot slip test, rotarod test, fear conditioning assay, etc.)
   

My lab has been working on the cellular and circuit mechanisms underlying memory, motor function, and epileptic seizures in normal and/or disease conditions. Our goal is to understand the cellular/neural circuit problems and then explore the treatment strategies for some neurodevelopmental neurological disorders (e.g., intellectual disability disorders, autism spectrum disorders). Recently, we found that deep brain stimulation of the fimbria-fornix rescues the learning and memory deficits, together with the enhancement of hippocampal synaptic plasticity and/or adult hippocampal neurogenesis in mouse models of Rett syndrome and CDKL5 deficiency disorder (Nature, 2015; Journal of Neuroscience 2021; Brain Stimulation, 2023).

Read about my lab’s recent breakthrough: https://nri.texaschildrens.org/synopsis/deep-brain-stimulation-induces-more-healthy-neurons-and-sustained-memory-benefits-rett

https://blogs.bcm.edu/2023/10/19/from-the-labs-deep-brain-stimulation-induces-more-healthy-neurons-and-sustained-memory-benefits-in-rett-animal-models/

• Qi Wang, Bin Tang, Shuang Hao, Zhenyu Wu, Tingting Yang, Jianrong Tang. 2023. Forniceal deep brain stimulation in a mouse model of Rett syndrome increases neurogenesis and hippocampal memory beyond the treatment period. Brain Stimul. 16: 1401-1411. PMID: 37704033.
• Lingjie He, Matthew S Caudill, Junzhan Jing, Wei Wang, Yaling Sun, Jianrong Tang, Xiaolong Jiang, Huda Y Zoghbi. 2022. A weakened recurrent circuit in the hippocampus of Rett syndrome mice disrupts long-term memory representations. Neuron. 110: 1689-1699. PMCID: 9930308
• Qi Wang, Nicola de Prisco, Jianrong Tang*, Vincenzo A Gennarino*. 2022. Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice. STAR Protoc, 3: 101245. PMCID: 8927982 (*co-correspondence)
• Qi Wang, Bin Tang, Jianrong Tang. 2021. Protocols for deep brain stimulation in the fimbria-fornix of freely moving mice. STAR Protoc, 3:101054. PMCID: PMC8715214.
• Shuang Hao, Qi Wang, Bin Tang, Zhenyu Wu, Tingting Yang, Jianrong Tang. 2021. CDKL5 Deficiency Augments Inhibitory Input into the Dentate Gyrus That Can Be Reversed by Deep Brain Stimulation. J. Neurosci., 41 : 9031-9046. PMCID: 8549531. (Featured Research, highlighted in Spectrum)
• Yingyao Shao, Yehezkel Sztainberg, Qi Wang, Sameer S. Bajikar, Alexander J. Trostle, Ying-Wooi Wan, Paymaan Jafar-nejad, Frank Rigo, Zhandong Liu, Jianrong Tang, Huda Y. Zoghbi. 2021. Antisense oligonucleotide therapy in a humanized mouse model of MECP2 duplication syndrome. Sci Transl Med. doi: 10.1126/scitranslmed.aaz7785. PMCID: 8976688
• Callison E Alcott, Hari Krishna Yalamanchili, Ping Ji, Meike E van der Heijden, Alexander Saltzman, Nathan Elrod, Ai Lin, Mei Leng, Bhoomi Bhatt, Shuang Hao, Qi Wang, Afaf Saliba, Jianrong Tang, Anna Malovannaya, Eric J Wagner, Zhandong Liu, Huda Y Zoghbi. 2020. Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation. eLIFE. doi: 10.7554/eLife.50895. PMCID: 7176433
• Wu Chen, Zhao-Lin Cai, Eugene S., Chao, Hongmei Chen, Colleen M Longley, Shuang Hao, Hsiao-Tuan Chao, Joo Hyun Kim, Jessica E. Messier, Huda Y. Zoghbi, Jianrong Tang, John W. Swann, Mingshan Xue. 2020. Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy. eLIFE.  doi: 10.7554/eLife.48705. PMCID: 7056272
• Thomas K Creson, Camilo Rojas, Ernie Hwaun, Thomas Vaissiere, Murat Kilinc, Andres Jimenez-Gomez, Jimmy Lloyd Holder Jr, Jianrong Tang, Laura L Colgin, Courtney A Miller and Gavin Rumbaug. 2019. Re-expression of SynGAP protein in adulthood improves translatable measures of brain function and behavior. eLIFE, pii: e46752. PMCID: 6504227
• Amy E. Pohodich, Hari Yalamanchili, Ayush T. Raman, Ying-Wooi Wan, Michael Gundry, Shuang Hao, Haijing Jin, Jianrong Tang, Zhandong Liu and Huda Y. Zoghbi. 2018. Forniceal deep brain stimulation induces gene expression and splicing changes that promote neurogenesis and plasticity. eLIFE, pii: e34031. PMCID: 5906096
• James P. Orengo, Meike E. van der Heijden, Shuang Hao, Jianrong Tang, Harry T. Orr, and Huda Y. Zoghbi. 2018. Motor neuron degeneration correlates with respiratory dysfunction in SCA1. Dis. Model. Mech., DOI: 10.1242/dmm.032623. PMCID: 5894948
• Vincenzo A. Gennarino, Elizabeth E. Palmer, Laura M. McDonell, Li Wang, Carolyn J. Adamski, Amanda Koire, Lauren See, Chun-An Chen, Christian P. Schaaf, Jill A. Rosenfeld, Jessica A. Panzer, Ute Moog, Shuang Hao, Ann Bye, Edwin P. Kirk, Pawel Stankiewicz, Amy M. Breman, Arran McBride, Tejaswi Kandula, Holly A. Dubbs, Rebecca Macintosh, Michael Cardamone, Ying Zhu, Kevin Ying, Kerith-Rae Dias, Megan T. Cho, Lindsay B. Henderson, Berivan Baskin, Paula Morris, Jiang Tao, Mark J. Cowley, Marcel E. Dinger, Tony Roscioli, Oana Caluseriu, Oksana Suchowersky, Rani K. Sachdev, Olivier Lichtarge, Jianrong Tang, Kym M. Boycott, J. Lloyd Holder, Jr., and Huda Y. Zoghbi. 2018. A Mild PUM1 Mutation Is Associated with Adult-Onset Ataxia, whereas Haploinsufficiency Causes Developmental Delay and Seizures. Cell. 172: 924-936. PMCID: 5832058
• Szu-Ying Yeh, Wei-Hsiang Huang, Wei Wang, Christopher S. Ward, Eugene S. Chao, Zhenyu Wu, Bin Tang, Jianrong Tang, Jenny J. Sun, Meike Esther van der Heijden, Paul A. Gray, Mingshan Xue, Russell S. Ray, Dejian Ren, and Huda Y. Zoghbi. 2017. Respiratory Network Stability and Modulatory Response to Substance P Require Nalcn. Neuron, 94: 294-303. PMCID: 5702257
• Cristian A. Lasagna-Reeves, Maria de Haro, Shuang Hao, Jeehye Park, Maxime W.C. Rousseaux, Ismael Al-Ramahi, Paymaan Jafar-Nejad, Luis Vilanova-Velez, Lauren See, Antonia De Maio, Larissa Nitschke, Zhenyu Wu, Juan C. Troncoso, Thomas F. Westbrook, Jianrong Tang, Juan Botas, and Huda.Y. Zoghbi. 2016. Reduction of Nuak1 decreases tau and reverses phenotypes in a tauopathy mouse model. Neuron, 92: 407-418. PMCID: 5745060
• Hui Lu, Ryan T. Ash, Lingjie He, Sara E. Kee, Wei Wang, Dinghui Yu, Shuang Hao, Xiangling Meng, Kerstin Ure, Aya Ito-Ishida, Bin Tang, Yaling Sun, Daoyun Ji, Jianrong Tang, Benjamin R. Arenkiel, Stelios M. Smirnakis, and Huda Y. Zoghbi. 2016. Loss and gain of MeCP2 cause similar hippocampal circuit dysfunction that is rescued by deep brain stimulation in a Rett syndrome mouse model. Neuron, 91: 739-747. PMCID: 5019177
• Kerstin Ure, Hui Lu, Wei Wang, Aya Ito-Ishida, Zhenyu Wu, Ling-jie He, Yehezkel Sztainberg, Wu Chen, Jianrong Tang, and Huda Y. Zoghbi. 2016. Restoration of Mecp2 expression in GABAergic neurons is sufficient to rescue multiple disease features in a mouse model of Rett Syndrome. eLIFE, pii: e14198. PMCID: 4946897
• Xiangling Meng, Wei Wang, Hui Lu, Ling-jie He, Wu Chen, Marta L. Fiorotto, Bin Tang, Eugene Chao, Jose A. Herrera, Michelle L. Seymour, Jeffrey L. Neul, Fred A. Pereira, Jianrong Tang, Mingshan Xue, and Huda Y. Zoghbi. 2016. Manipulations of MeCP2 in glutamatergic neurons highlight their contributions to Rett and other neurological disorders. eLIFE, pii: e14199. PMCID: 4946906
• Yehezkel Sztainberg, Hongmei Chen, John W. Swann, Shuang Hao, Bin Tang, Zhenyu Wu, Jianrong Tang, Ying-Wooi Wan, Zhandong Liu, Frank Rigo and Huda Y. Zoghbi. 2015. Reversal of phenotypes in MECP2 duplication mice using genetic rescue or antisense oligos. Nature, 528: 123-126. PMCID: 4839300
• Shuang Hao, Bin Tang, Zhenyu Wu, Kerstin Ure, Yaling Sun, Huifang Tao, Yan Gao, Akash J. Patel, Daniel J. Curry, Rodney C. Samaco, Huda Y. Zoghbi and Jianrong Tang. 2015. Forniceal deep brain stimulation rescues hippocampal memory in Rett syndrome mice. Nature, 526: 430-434. (Highlighted in News and View: Nature, 526: 331-332; Nature Review Neurology, 11: 666, 2015; Faculty 1000, and internet press releases). PMCID: 4828032
• Daniel Jenson, Kechun Yang, Alexandra Acevedo-Rodriguez, Amber Levine, John I. Broussard, Jianrong Tang, John A. Dani. 2014. Dopamine and norepinephrine receptors participate in methylphenidate enhancement of in vivo hippocampal synaptic plasticity. Neuropharmacology, 90: 23-32. PMCID: 4533926
• Kihoon Han, J. Lloyd Holder, Jr Christian P. Schaaf, Hui Lu, Hongmei Chen, Hyojin Kang, Jianrong Tang, ZhenyuWu, Shuang Hao, SauWai Cheung, Peng Yu, Hao Sun, Amy M. Breman, Ankita Patel, Hui-Chen Lu & Huda Y. Zoghbi. 2013. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature, 503: 72-77. PMCID: 3923348