!

COVID-19 Response 

Access our COVID-19 Response homepage, with more information and resources during the COVID-19 pandemic, including what to do if you’re experiencing symptoms.

Weather Update

Baylor College of Medicine will be open Wednesday and Baylor Medicine will be seeing patients. Please prioritize your safety and take appropriate safety precautions if driving in any high water. View message. 

Dan L Duncan Comprehensive Cancer Center

CPRIT Cancer Proteomics and Metabolomics

Master
Heading

Overview

Content

The Cancer Prevention and Research Institute of Texas is supporting a Cancer Proteomic and Metabolomic Core Facility that provides consultation, instrumentation, technical support and data analysis for cancer research projects. The facility operates as a fee-for-service core with very competitive pricing subsidized by the CPRIT grant. The core facility accepts requests for cancer related research from Baylor College of Medicine faculty as a priority.

Heading

Services

Content

The core facility provides the following technology platforms as services. Detailed protocols for each platform and policies for operation can be found with attached links:

Heading

MS Proteomics

Content

Mass spectrometry identification of protein complexes and their interacting protein networks and untargeted label-free proteomic profiling. This work is conducted under the direction of Drs. Anna Malovannaya and Sung Yun Jung primarily with a Thermo Fisher Orbitrap mass spectrometers.

Learn more about MS Proteomics.

Heading

Reverse Phase Protein Array

Content

Antibody-based reverse-phase protein arrays (RPPA) to quantitatively analyze specific protein pathways and their activation states with large sample sets (tissue or cells). Approximately 240 validated antibodies are available for RPPA. This work is conducted by Dr. Shixia Huang using an Aushon 2470 robotic protein arrayer and a DAKO high throughput auto-slide immunostainer.

Learn more about Reverse Phase Protein Array.

Heading

Metabolomics

Content

Targeted measurement of steady-state metabolites (up to 700 known metabolites) untargeted profiling, lipidomics and metabolic flux. This work is conducted by Drs. Arun Sreekumar and Nagireddy Putluri using multiple Agilent mass spectrometers and a SCIEX TOF mass spectrometer.

Learn more about Metabolomics.

Heading

Multi-Omics Data Analysis Core

Content

Consultation on design of projects and data analysis are also provided as Core Services. Data analysis is performed with customized software tools developed for each of the three technology platforms.

Learn more about Multi-Omics Data Analysis.

Heading

Project Requirements

Content

All projects will require submission of a two-page request for project (RFP) application that will be reviewed for feasibility, appropriateness for the available technology platforms, cancer relevance and scientific merit. Approved RFPs will be prioritized, scheduled accordingly, and specifics of each project will be designed prior to initiation. The PI of the requesting lab is required to attend the initial planning meeting of the approved project. Pricing for projects will be provided upon request. At the completion of each project an additional consultation will be provided to review the analyzed data.

Publications that result from the work of the CPRIT Core facility require acknowledgement of the CPRIT Core Facility grant (Proteomics and Metabolomics Core Facility RP 170005).

Heading

Leader and Core Director:

Content

Principle Investigator and Core Director:
Dean P. Edwards, Ph.D.
deane@bcm.edu

MS Proteomics
Leader:  Anna Malovannaya, Ph.D.
malvann@bcm.edu
Co-Leader:  Sun Jung, Ph.D.
Jyjung@bcm.edu

Reverse Phase Protein Array (RPPA)
Leader: Shixia Huang, Ph.D.
shixiah@bcm.edu

Metabolomics
Leader: Arun Sreekumar, Ph.D.
Co-Leader: Nagireddy Putluri, Ph.D.
metabolomicscore@bcm.edu

Multi-Omics Data Analysis
Leader: Christian Coarfa, Ph.D.
coarfa@bcm.edu

Heading

In The News

Content

CPRIT awards Baylor $5 million grant for core facility support in proteomics and metabolomics