The measurements are performed in a CNRF procedure room. Each mouse is lightly anesthetized by placing it in a chamber with isoflurane (5% and 95% oxygen). As soon as the mouse is sedated it is oriented carefully on a disposable PIXImus measuring tray for proper alignment and is fitted with a loosely fitting nose cone for administration of isoflurane (2% isoflurane) until the measurement is complete. If you prefer, you may use an injectable anesthetic. The mouse is inserted into the instrument for measurement (approx. 5 minutes) and then returned to a cage to recover under observation. Once fully recovered the mouse can be returned to its home cage.
There are some systematic errors in the lean and fat mass values obtained by PIXImus that vary depending on the instrument model and software version (see References). To provide more accurate measurements, we have derived some correction equations to apply to the data generated by the CNRC PIXImus. The procedure and further information can be found below
Download calculator for adjusting PIXImus body composition data to chemical analysis-comparable values (Microsoft Excel spreadsheet)