Timothy Palzkill Lab

Palzkill Lab Publications


Tang SA, Fults A, Boyd SR, Gattu N, Tran KA, Fan J, MacKenzie KR, Palzkill T, Young DW, Chamakuri S. Expanding Complex Morpholines Using Systematic Chemical Diversity. Org Lett. 2024 Mar 20. PMID: 38506470.

Judge A, Sankaran B, Hu L, Palaniappan M, Birgy A, Prasad BVV, Palzkill T. Network of epistatic interactions in an enzyme active site revealed by large-scale deep mutational scanning. Proc Natl Acad Sci U S A. 2024 Mar 19; 121(12):e2313513121. PMID: 38483989

Birgy A, Nnabuife C, Palzkill T. The mechanism of ceftazidime and cefiderocol hydrolysis by D179Y variants of KPC carbapenemases is similar and involves the formation of a long-lived covalent intermediate. Antimicrob Agents Chemother. 2024 Mar 06; 68(3):e0110823. PMID: 38259088; PMCID: PMC10916376

Park S, Fan J, Chamakuri S, Palaniappan M, Sharma K, Qin X, Wang J, Tan Z, Judge A, Hu L, Sankaran B, Li F, Prasad BVV, Matzuk MM, Palzkill T. Exploiting the Carboxylate-Binding Pocket of ?-Lactamase Enzymes Using a Focused DNA-Encoded Chemical Library. J Med Chem. 2024 Jan 11; 67(1):620-642. PMID: 38117688.

Sun Z, Lin H, Hu L, Neetu N, Sankaran B, Wang J, Prasad BVV, Palzkill T. Klebsiella pneumoniae carbapenemase variant 44 (KPC-44) acquires ceftazidime-avibactam resistance by altering the conformation of active site loops. J Biol Chem. 2023 Nov 22; 105493. PMID: 38000656.

Jimmidi R, Chamakuri S, Lu S, Ucisik MN, Chen PJ, Bohren KM, Moghadasi SA, Versteeg L, Nnabuife C, Li JY, Qin X, Chen YC, Faver JC, Nyshadham P, Sharma KL, Sankaran B, Judge A, Yu Z, Li F, Pollet J, Harris RS, Matzuk MM, Palzkill T, Young DW. DNA-encoded chemical libraries yield non-covalent and non-peptidic SARS-CoV-2 main protease inhibitors. Commun Chem. 2023 Aug 4;6(1):164. PMID: 37542196; PMCID: PMC10403511

Materon IC, Palzkill T. Structural biology of MCR-1-mediated resistance to polymyxin antibiotics. Curr Opin Struct Biol. 2023 Jul 01; 82:102647. PMID: 37399693.

Su L, Huang W, Neill FH, Estes MKAtmar RL, Palzkill T. Mapping human norovirus antigens during infection reveals the breadth of the humoral immune response. NPJ Vaccines. 2023 Jun 06; 8(1):87. PMID: 37280322; PMCID: PMC10242225.

Moghadasi SA, Heilmann E, Khalil AM, Nnabuife C, Kearns FL, Ye C, Moraes SN, Costacurta F, Esler MA, Aihara H, von Laer D, Martinez-Sobrido L, Palzkill T, Amaro RE, Harris RS. Transmissible SARS-CoV-2 variants with resistance to clinical protease inhibitors. Sci Adv. 2023 03 29; 9(13):eade8778. PMID: 36989354; PMCID: PMC10058310.

Sankaran B, Lu S, Montoya M, Hu L, Neetu N, Prasad BVV, Palzkill T. Mutagenesis and structural analysis reveal the CTX-M ?-lactamase active site is optimized for cephalosporin catalysis and drug resistance. J Biol Chem. 2023 05; 299(5):104630. PMID: 36963495; PMCID: PMC10139949.

Judge A, Hu L, Sankaran B, Van Riper J, Venkataram Prasad BV, Palzkill T. Mapping the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M ?-lactamase. Commun Biol. 2023 01 12; 6(1):35. PMID: 36635385; PMCID: PMC9837174.

Moghadasi SA, Heilmann E, Khalil AM, Nnabuife C, Kearns FL, Ye C, Moraes SN, Costacurta F, Esler MA, Aihara H, von Laer D, Martinez-Sobrido L, Palzkill T, Amaro RE, Harris RS. Transmissible SARS-CoV-2 variants with resistance to clinical protease inhibitors. bioRxiv. 2022 Dec 26. PMID: 35982678; PMCID: PMC9387136.

Yin C, Song Z, Tian H, Palzkill T, Tao P. Unveiling the structural features that regulate carbapenem deacylation in KPC-2 through QM/MM and interpretable machine learning. Phys Chem Chem Phys. 2022 Dec 20. PMID: 36537692.

Lu S, Hu L, Lin H, Judge A, Rivera P, Palaniappan M, Sankaran B, Wang J, Prasad BVV, Palzkill T. An active site loop toggles between conformations to control antibiotic hydrolysis and inhibition potency for CTX-M ?-lactamase drug-resistance enzymes. Nat Commun. 2022 Nov 07; 13(1):6726. PMID: 36344533; PMCID: PMC9640584.

Rossi MA, Palzkill T, Almeida FCL, Vila AJ. Slow Protein Dynamics Elicits New Enzymatic Functions by Means of Epistatic Interactions. Mol Biol Evol. 2022 Oct 07; 39(10). PMID: 36136729; PMCID: PMC9547502.

Sun Z, Su L, Cotroneo N, et al. Evaluation of Tebipenem Hydrolysis by ß-Lactamases Prevalent in Complicated Urinary Tract Infections. Antimicrob Agents Chemother. 2022 May 02; e0239621. PMID: 35491852.

Bradford PA, Bonomo RA, Bush K, et al. Consensus on ß-Lactamase Nomenclature. Antimicrob Agents Chemother. 2022 04 19; 66(4):e0033322. PMID: 35380458.

Boragine DM, Huang W, Su LH, et al. Deep Sequencing of a Systematic Peptide Library Reveals Conformationally-Constrained Protein Interface Peptides that Disrupt a Protein-Protein Interaction. Chembiochem. 2022 02 04; 23(3):e202100504. PMID: 34821011.

Taylor DM, Anglin J, Hu L, et al. Unique Diacidic Fragments Inhibit the OXA-48 Carbapenemase and Enhance the Killing of Escherichia coli Producing OXA-48. ACS Infect Dis. 2021 12 10; 7(12):3345-3354. PMID: 34817169.

Sun Z, Palzkill T. Deep Mutational Scanning Reveals the Active-Site Sequence Requirements for the Colistin Antibiotic Resistance Enzyme MCR-1. mBio. 2021 12 21; 12(6):e0277621. PMID: 34781730.

Song Z, Trozzi F, Palzkill T, et al. QM/MM modeling of class A ß-lactamases reveals distinct acylation pathways for ampicillin and cefalexin. Org Biomol Chem. 2021 11 03; 19(42):9182-9189. PMID: 34647114.

Chamakuri S, Lu S, Ucisik MN, et al. DNA-encoded chemistry technology yields expedient access to SARS-CoV-2 Mpro inhibitors. Proc Natl Acad Sci U S A. 2021 09 07; 118(36). PMID: 34426525.

Furey IM, Mehta SC, Sankaran B, et al. Local interactions with the Glu166 base and the conformation of an active site loop play key roles in carbapenem hydrolysis by the KPC-2 ß-lactamase. J Biol Chem. 2021 Jan-Jun; 296:100799. PMID: 34022225.

Rincon S, Carvajal LP, Gomez-Villegas SI, et al. A Test for the Rapid Detection of the Cefazolin Inoculum Effect in Methicillin-Susceptible Staphylococcus aureus. J Clin Microbiol. 2021 03 19; 59(4). PMID: 33536292.

Stojanoski V, Hu L, Sankaran B, et al. Mechanistic Basis of OXA-48-like ß-Lactamases' Hydrolysis of Carbapenems. ACS Infect Dis. 2021 02 12; 7(2):445-460. PMID: 33492952.

Mehta SC, Furey IM, Pemberton OA, et al. KPC-2 ß-lactamase enables carbapenem antibiotic resistance through fast deacylation of the covalent intermediate. J Biol Chem. 2021 Jan-Jun; 296:100155. PMID: 33273017.

Huang W, Soeung V, Boragine DM, et al. High-Resolution Mapping of Human Norovirus Antigens via Genomic Phage Display Library Selections and Deep Sequencing. J Virol. 2020 12 09; 95(1). PMID: 33055250.

Soeung V, Lu S, Hu L, et al. A drug-resistant ß-lactamase variant changes the conformation of its active-site proton shuttle to alter substrate specificity and inhibitor potency. J Biol Chem. 2020 12 25; 295(52):18239-18255. PMID: 33109613.

Lam FW, Brown CA, Valladolid C, et al. The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium. PLoS One. 2020; 15(10):e0240164. PMID: 33048962.

Huang W, Soeung V, Boragine DM, et al. Mapping Protein-Protein Interaction Interface Peptides with Jun-Fos Assisted Phage Display and Deep Sequencing. ACS Synth Biol. 2020 07 17; 9(7):1882-1896. PMID: 32502338.

Zhu L, Olsen RJ, Beres SB, et al. Streptococcus pyogenes genes that promote pharyngitis in primates. JCI Insight. 2020 06 04; 5(11). PMID: 32493846.

Brown CA, Hu L, Sun Z, et al. Antagonism between substitutions in ß-lactamase explains a path not taken in the evolution of bacterial drug resistance. J Biol Chem. 2020 05 22; 295(21):7376-7390. PMID: 32299911.

Valladolid C, Martinez-Vargas M, Sekhar N, et al. Modulating the rate of fibrin formation and clot structure attenuates microvascular thrombosis in systemic inflammation. Blood Adv. 2020 04 14; 4(7):1340-1349. PMID: 32259201.

Musser JM, Beres SB, Zhu L, et al. Reduced In Vitro Susceptibility of Streptococcus pyogenes to ß-Lactam Antibiotics Associated with Mutations in the pbp2x Gene Is Geographically Widespread. J Clin Microbiol. 2020 03 25; 58(4). PMID: 31996443.

Taylor DM, Anglin J, Park S, et al. Identifying Oxacillinase-48 Carbapenemase Inhibitors Using DNA-Encoded Chemical Libraries. ACS Infect Dis. 2020 05 08; 6(5):1214-1227. PMID: 32182432.

Mack AR, Barnes MD, Taracila MA, et al. A Standard Numbering Scheme for Class C ß-Lactamases. Antimicrob Agents Chemother. 2020 02 21; 64(3). PMID: 31712217.

Lu S, Soeung V, Nguyen HAT, et al. Development and Evaluation of a Novel Protein-Based Assay for Specific Detection of KPC ß-Lactamases from Klebsiella pneumoniae Clinical Isolates. mSphere. 2020 01 08; 5(1). PMID: 31915233.

Sutton MN, Lu Z, Li YC, et al. DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters. Cell Rep. 2019 12 10; 29(11):3448-3459.e6. PMID: 31825828.

Beggs GA, Zalucki YM, Brown NG, et al. Structural, Biochemical, and In Vivo Characterization of MtrR-Mediated Resistance to Innate Antimicrobials by the Human Pathogen Neisseria gonorrhoeae. J Bacteriol. 2019 10 15; 201(20). PMID: 31331979.

Khan A, Tran TT, Rios R, et al. Extensively Drug-Resistant Pseudomonas aeruginosa ST309 Harboring Tandem Guiana Extended Spectrum ß-Lactamase Enzymes: A Newly Emerging Threat in the United States. Open Forum Infect Dis. 2019 Jul; 6(7):ofz273. PMID: 31281867.

Sutton MN, Huang GY, Liang X, et al. DIRAS3-Derived Peptide Inhibits Autophagy in Ovarian Cancer Cells by Binding to Beclin1. Cancers (Basel). 2019 Apr 18; 11(4). PMID: 31003488.

Viskovska MA, Zhao B, Shanker S, et al. GII.4 Norovirus Protease Shows pH-Sensitive Proteolysis with a Unique Arg-His Pairing in the Catalytic Site. J Virol. 2019 03 15; 93(6). PMID: 30626675.

Wang L, Pang K, Han K, et al. An autism-linked missense mutation in SHANK3 reveals the modularity of Shank3 function. Mol Psychiatry. 2020 10; 25(10):2534-2555. PMID: 30610205.

Jiang X, Zhang C, Chen J, et al. Quantitative Real-Time Imaging of Glutathione with Subcellular Resolution. Antioxid Redox Signal. 2019 06 01; 30(16):1900-1910. PMID: 30358421.

Sun Z, Hu L, Sankaran B, et al. Differential active site requirements for NDM-1 ß-lactamase hydrolysis of carbapenem versus penicillin and cephalosporin antibiotics. Nat Commun. 2018 10 30; 9(1):4524. PMID: 30375382.

Patel MP, Hu L, Brown CA, et al. Synergistic effects of functionally distinct substitutions in ß-lactamase variants shed light on the evolution of bacterial drug resistance. J Biol Chem. 2018 11 16; 293(46):17971-17984. PMID: 30275013.

Palzkill T. Structural and Mechanistic Basis for Extended-Spectrum Drug-Resistance Mutations in Altering the Specificity of TEM, CTX-M, and KPC ß-lactamases. Front Mol Biosci. 2018; 5:16. PMID: 29527530.

Chen J, Jiang X, Zhang C, et al. Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria. ACS Sens. 2017 Sep 22; 2(9):1257-1261. PMID: 28809477.

Patel MP, Hu L, Stojanoski V, et al. The Drug-Resistant Variant P167S Expands the Substrate Profile of CTX-M ß-Lactamases for Oxyimino-Cephalosporin Antibiotics by Enlarging the Active Site upon Acylation. Biochemistry. 2017 07 11; 56(27):3443-3453. PMID: 28613873.

Adamski CJ, Palzkill T. Systematic substitutions at BLIP position 50 result in changes in binding specificity for class A ß-lactamases. BMC Biochem. 2017 03 06; 18(1):2. PMID: 28264645.

Adamski CJ, Palzkill T. BLIP-II Employs Differential Hotspot Residues To Bind Structurally Similar Staphylococcus aureus PBP2a and Class A ß-Lactamases. Biochemistry. 2017 02 28; 56(8):1075-1084. PMID: 28182405.

Hurwitz AM, Huang W, Kou B, et al. Identification and Characterization of Single-Chain Antibodies that Specifically Bind GI Noroviruses. PLoS One. 2017; 12(1):e0170162. PMID: 28095447.

Hurwitz AM, Huang W, Estes MK, et al. Deep sequencing of phage-displayed peptide libraries reveals sequence motif that detects norovirus. Protein Eng Des Sel. 2017 Feb; 30(2):129-139. PMID: 28035012.

Chow DC, Rice K, Huang W, et al. Engineering Specificity from Broad to Narrow: Design of a ß-Lactamase Inhibitory Protein (BLIP) Variant That Exclusively Binds and Detects KPC ß-Lactamase. ACS Infect Dis. 2016 12 09; 2(12):969-979. PMID: 27756125.

Stojanoski V, Sankaran B, Prasad BV, et al. Structure of the catalytic domain of the colistin resistance enzyme MCR-1. BMC Biol. 2016 Sep 21; 14(1):81. PMID: 27655155.

Sun Z, Mehta SC, Adamski CJ, et al. Deep Sequencing of Random Mutant Libraries Reveals the Active Site of the Narrow Specificity CphA Metallo-ß-Lactamase is Fragile to Mutations. Sci Rep. 2016 09 12; 6:33195. PMID: 27616327.

Prasad BV, Shanker S, Muhaxhiri Z, et al. Antiviral targets of human noroviruses. Curr Opin Virol. 2016 06; 18:117-25. PMID: 27318434.

Jacoby GA, Bonomo RA, Bradford PA, et al. Comment on: Resistance gene naming and numbering: is it a new gene or not? J Antimicrob Chemother. 2016 09; 71(9):2677-8. PMID: 27261266.

Stojanoski V, Adamski CJ, Hu L, et al. Removal of the Side Chain at the Active-Site Serine by a Glycine Substitution Increases the Stability of a Wide Range of Serine ß-Lactamases by Relieving Steric Strain. Biochemistry. 2016 05 03; 55(17):2479-90. PMID: 27073009.

Song X, Chen J, Zhao M, et al. Development of potent small-molecule inhibitors to drug the undruggable steroid receptor coactivator-3. Proc Natl Acad Sci U S A. 2016 May 03; 113(18):4970-5. PMID: 27084884.

Kou B, Huang W, Neill FH, et al. Norovirus Antigen Detection with a Combination of Monoclonal and Single-Chain Antibodies. J Clin Microbiol. 2015 Dec; 53(12):3916-8. PMID: 26447115.

Patel MP, Fryszczyn BG, Palzkill T. Characterization of the global stabilizing substitution A77V and its role in the evolution of CTX-M ß-lactamases. Antimicrob Agents Chemother. 2015 Nov; 59(11):6741-8. PMID: 26282414.

Wang L, Yu Y, Chow DC, et al. Characterization of a Steroid Receptor Coactivator Small Molecule Stimulator that Overstimulates Cancer Cells and Leads to Cell Stress and Death. Cancer Cell. 2015 Aug 10; 28(2):240-52. PMID: 26267537.

Mehta SC, Rice K, Palzkill T. Natural Variants of the KPC-2 Carbapenemase have Evolved Increased Catalytic Efficiency for Ceftazidime Hydrolysis at the Cost of Enzyme Stability. PLoS Pathog. 2015 Jun; 11(6):e1004949. PMID: 26030609.

Stojanoski V, Chow DC, Fryszczyn B, et al. Structural Basis for Different Substrate Profiles of Two Closely Related Class D ß-Lactamases and Their Inhibition by Halogens. Biochemistry. 2015 Jun 02; 54(21):3370-80. PMID: 25938261.

Abriata LA, Palzkill T, Dal Peraro M. How structural and physicochemical determinants shape sequence constraints in a functional enzyme. PLoS One. 2015; 10(2):e0118684. PMID: 25706742.

Stojanoski V, Chow DC, Hu L, et al. A triple mutant in the O-loop of TEM-1 ß-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis. J Biol Chem. 2015 Apr 17; 290(16):10382-94. PMID: 25713062.

Humphreys AF, Tan J, Peng R, et al. Generation and characterization of antibodies against Asian elephant (Elephas maximus) IgG, IgM, and IgA. PLoS One. 2015; 10(2):e0116318. PMID: 25658336.

Adamski CJ, Cardenas AM, Brown NG, et al. Molecular basis for the catalytic specificity of the CTX-M extended-spectrum ß-lactamases. Biochemistry. 2015 Jan 20; 54(2):447-57. PMID: 25489790.

He B, Lanz RB, Fiskus W, et al. GATA2 facilitates steroid receptor coactivator recruitment to the androgen receptor complex. Proc Natl Acad Sci U S A. 2014 Dec 23; 111(51):18261-6. PMID: 25489091.

Kou B, Crawford SE, Ajami NJ, et al. Characterization of cross-reactive norovirus-specific monoclonal antibodies. Clin Vaccine Immunol. 2015 Feb; 22(2):160-7. PMID: 25428247.

Viskovska M, Anish R, Hu L, et al. Probing the sites of interactions of rotaviral proteins involved in replication. J Virol. 2014 Nov; 88(21):12866-81. PMID: 25165107.

Long SW, Olsen RJ, Mehta SC, et al. PBP2a mutations causing high-level Ceftaroline resistance in clinical methicillin-resistant Staphylococcus aureus isolates. Antimicrob Agents Chemother. 2014 Nov; 58(11):6668-74. PMID: 25155594.

Fryszczyn BG, Adamski CJ, Brown NG, et al. Role of ß-lactamase residues in a common interface for binding the structurally unrelated inhibitory proteins BLIP and BLIP-II. Protein Sci. 2014 Sep; 23(9):1235-46. PMID: 24947275.

Huang W, Samanta M, Crawford SE, et al. Identification of human single-chain antibodies with broad reactivity for noroviruses. Protein Eng Des Sel. 2014 Oct; 27(10):339-49. PMID: 24946948.

Yan F, Yu Y, Chow DC, et al. Identification of verrucarin a as a potent and selective steroid receptor coactivator-3 small molecule inhibitor. PLoS One. 2014; 9(4):e95243. PMID: 24743578.

Wang Y, Lonard DM, Yu Y, et al. Bufalin is a potent small-molecule inhibitor of the steroid receptor coactivators SRC-3 and SRC-1. Cancer Res. 2014 Mar 01; 74(5):1506-1517. PMID: 24390736.

Dave K, Palzkill T, Pratt RF. Neutral ß-Lactams Inactivate High Molecular Mass Penicillin-Binding Proteins of Class B1, Including PBP2a of MRSA. ACS Med Chem Lett. 2014 Feb 13; 5(2):154-7. PMID: 24900789.

Deng L, Muhaxhiri Z, Estes MK, et al. Synthesis, Activity and Structure-Activity Relationship of Noroviral Protease Inhibitors. Medchemcomm. 2013 Oct; 4(10). PMID: 24244836.

Brown NG, Chow DC, Ruprecht KE, et al. Identification of the ß-lactamase inhibitor protein-II (BLIP-II) interface residues essential for binding affinity and specificity for class A ß-lactamases. J Biol Chem. 2013 Jun 14; 288(24):17156-66. PMID: 23625930.

Brown NG, Chow DC, Palzkill T. BLIP-II is a highly potent inhibitor of Klebsiella pneumoniae carbapenemase (KPC-2). Antimicrob Agents Chemother. 2013 Jul; 57(7):3398-401. PMID: 23587951.

Rogers JD, Ajami NJ, Fryszczyn BG, et al. Identification and characterization of a peptide affinity reagent for detection of noroviruses in clinical samples. J Clin Microbiol. 2013 Jun; 51(6):1803-8. PMID: 23554202.

Muhaxhiri Z, Deng L, Shanker S, et al. Structural basis of substrate specificity and protease inhibition in Norwalk virus. J Virol. 2013 Apr; 87(8):4281-92. PMID: 23365454.

Palzkill T. Metallo-ß-lactamase structure and function. Ann N Y Acad Sci. 2013 Jan; 1277:91-104. PMID: 23163348.

Deng Z, Huang W, Bakkalbasi E, et al. Deep sequencing of systematic combinatorial libraries reveals ß-lactamase sequence constraints at high resolution. J Mol Biol. 2012 Dec 07; 424(3-4):150-67. PMID: 23017428.

McWilliams BD, Palzkill T, Weinstock GM, et al. Identification of novel and cross-species seroreactive proteins from Bacillus anthracis using a ligation-independent cloning-based, SOS-inducible expression system. Microb Pathog. 2012 Nov-Dec; 53(5-6):250-8. PMID: 22975444.

Horton LB, Shanker S, Mikulski R, et al. Mutagenesis of zinc ligand residue Cys221 reveals plasticity in the IMP-1 metallo-ß-lactamase active site. Antimicrob Agents Chemother. 2012 Nov; 56(11):5667-77. PMID: 22908171.

Chen P, Horton LB, Mikulski RL, et al. 2-Substituted 4,5-dihydrothiazole-4-carboxylic acids are novel inhibitors of metallo-ß-lactamases. Bioorg Med Chem Lett. 2012 Oct 01; 22(19):6229-32. PMID: 22921080.

Hu L, Chow DC, Patton JT, et al. Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5' GG Sequence for RTPase Activity. J Virol. 2012 Oct; 86(19):10547-57. PMID: 22811529.

Cai G, Deng L, Fryszczyn BG, et al. Thermodynamic Investigation of Inhibitor Binding to 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase. ACS Med Chem Lett. 2012 Jun 14; 3(6):496-500. PMID: 23050057.

Shelburne SA, Olsen RJ, Makthal N, et al. An amino-terminal signal peptide of Vfr protein negatively influences RopB-dependent SpeB expression and attenuates virulence in Streptococcus pyogenes. Mol Microbiol. 2011 Dec; 82(6):1481-95. PMID: 22040048.

Wang Y, Lonard DM, Yu Y, et al. Small molecule inhibition of the steroid receptor coactivators, SRC-3 and SRC-1. Mol Endocrinol. 2011 Dec; 25(12):2041-53. PMID: 22053001.

Yuan J, Cardenas AM, Gilbert HF, et al. Determination of the amino acid sequence requirements for catalysis by the highly proficient orotidine monophosphate decarboxylase. Protein Sci. 2011 Nov; 20(11):1891-906. PMID: 21898650.

Fryszczyn BG, Brown NG, Huang W, et al. Use of periplasmic target protein capture for phage display engineering of tight-binding protein-protein interactions. Protein Eng Des Sel. 2011 Nov; 24(11):819-28. PMID: 21900304.

Brown NG, Horton LB, Huang W, et al. Analysis of the functional contributions of Asn233 in metallo-ß-lactamase IMP-1. Antimicrob Agents Chemother. 2011 Dec; 55(12):5696-702. PMID: 21896903.

Brown NG, Chow DC, Sankaran B, et al. Analysis of the binding forces driving the tight interactions between beta-lactamase inhibitory protein-II (BLIP-II) and class A beta-lactamases. J Biol Chem. 2011 Sep 16; 286(37):32723-35. PMID: 21775426.

Kim JJ, Casteel DE, Huang G, et al. Co-crystal structures of PKG Iß (92-227) with cGMP and cAMP reveal the molecular details of cyclic-nucleotide binding. PLoS One. 2011 Apr 19; 6(4):e18413. PMID: 21526164.

Yuan J, Chow DC, Huang W, et al. Identification of a ß-lactamase inhibitory protein variant that is a potent inhibitor of Staphylococcus PC1 ß-lactamase. J Mol Biol. 2011 Mar 11; 406(5):730-44. PMID: 21238457.

Gao H, Wang X, Yang ZK, et al. Physiological roles of ArcA, Crp, and EtrA and their interactive control on aerobic and anaerobic respiration in Shewanella oneidensis. PLoS One. 2010 Dec 28; 5(12):e15295. PMID: 21203399.

Brown NG, Pennington JM, Huang W, et al. Multiple global suppressors of protein stability defects facilitate the evolution of extended-spectrum TEM ß-lactamases. J Mol Biol. 2010 Dec 17; 404(5):832-46. PMID: 20955714.

Brown NG, Shanker S, Prasad BV, et al. Structural and biochemical evidence that a TEM-1 beta-lactamase N170G active site mutant acts via substrate-assisted catalysis. J Biol Chem. 2009 Nov 27; 284(48):33703-12. PMID: 19812041.

Marciano DC, Brown NG, Palzkill T. Analysis of the plasticity of location of the Arg244 positive charge within the active site of the TEM-1 beta-lactamase. Protein Sci. 2009 Oct; 18(10):2080-9. PMID: 19672877.

Yuan J, Huang W, Chow DC, et al. Fine mapping of the sequence requirements for binding of beta-lactamase inhibitory protein (BLIP) to TEM-1 beta-lactamase using a genetic screen for BLIP function. J Mol Biol. 2009 Jun 05; 389(2):401-12. PMID: 19389404.

Wang J, Palzkill T, Chow DC. Structural insight into the kinetics and DeltaCp of interactions between TEM-1 beta-lactamase and beta-lactamase inhibitory protein (BLIP). J Biol Chem. 2009 Jan 02; 284(1):595-609. PMID: 18840610.

Marciano DC, Pennington JM, Wang X, et al. Genetic and structural characterization of an L201P global suppressor substitution in TEM-1 beta-lactamase. J Mol Biol. 2008 Dec 05; 384(1):151-64. PMID: 18822298.

Gao H, Pattison D, Yan T, et al. Generation and validation of a Shewanella oneidensis MR-1 clone set for protein expression and phage display. PLoS One. 2008 Aug 20; 3(8):e2983. PMID: 18714347.

Wang X, Gao H, Shen Y, et al. A high-throughput percentage-of-binding strategy to measure binding energies in DNA-protein interactions: application to genome-scale site discovery. Nucleic Acids Res. 2008 Sep; 36(15):4863-71. PMID: 18653527.

Titz B, Rajagopala SV, Goll J, et al. The binary protein interactome of Treponema pallidum--the syphilis spirochete. PLoS One. 2008 May 28; 3(5):e2292. PMID: 18509523.

Matejková P, Strouhal M, Smajs D, et al. Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays. BMC Microbiol. 2008 May 15; 8:76. PMID: 18482458.

Brinkman MB, McGill MA, Pettersson J, et al. A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin. Infect Immun. 2008 May; 76(5):1848-57. PMID: 18332212.

Gao H, Wang X, Yang ZK, et al. Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses. BMC Genomics. 2008 Jan 25; 9:42. PMID: 18221523.

Hyser JM, Zeng CQ, Beharry Z, et al. Epitope mapping and use of epitope-specific antisera to characterize the VP5* binding site in rotavirus SA11 NSP4. Virology. 2008 Mar 30; 373(1):211-28. PMID: 18164740.

Rajagopala SV, Titz B, Goll J, et al. The protein network of bacterial motility. Mol Syst Biol. 2007; 3:128. PMID: 17667950.

Marciano DC, Karkouti OY, Palzkill T. A fitness cost associated with the antibiotic resistance enzyme SME-1 beta-lactamase. Genetics. 2007 Aug; 176(4):2381-92. PMID: 17565956.

Wang J, Zhang Z, Palzkill T, et al. Thermodynamic investigation of the role of contact residues of beta-lactamase-inhibitory protein for binding to TEM-1 beta-lactamase. J Biol Chem. 2007 Jun 15; 282(24):17676-84. PMID: 17430899.

Holloway AK, Palzkill T, Bull JJ. Experimental evolution of gene duplicates in a bacterial plasmid model. J Mol Evol. 2007 Feb; 64(2):215-22. PMID: 17211548.

Cushman I, Palzkill T, Moore MS. Using peptide arrays to define nuclear carrier binding sites on nucleoporins. Methods. 2006 Aug; 39(4):329-41. PMID: 16908185.

Brinkman MB, McKevitt M, McLoughlin M, et al. Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome. J Clin Microbiol. 2006 Mar; 44(3):888-91. PMID: 16517872.

Majiduddin FK, Palzkill T. Amino acid residues that contribute to substrate specificity of class A beta-lactamase SME-1. Antimicrob Agents Chemother. 2005 Aug; 49(8):3421-7. PMID: 16048956.

McKevitt M, Brinkman MB, McLoughlin M, et al. Genome scale identification of Treponema pallidum antigens. Infect Immun. 2005 Jul; 73(7):4445-50. PMID: 15972547.

Adediran SA, Zhang Z, Nukaga M, et al. The D-methyl group in beta-lactamase evolution: evidence from the Y221G and GC1 mutants of the class C beta-lactamase of Enterobacter cloacae P99. Biochemistry. 2005 May 24; 44(20):7543-52. PMID: 15895997.

Beharry Z, Palzkill T. Functional analysis of active site residues of the fosfomycin resistance enzyme FosA from Pseudomonas aeruginosa. J Biol Chem. 2005 May 06; 280(18):17786-91. PMID: 15741169.

Smajs D, McKevitt M, Howell JK, et al. Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection. J Bacteriol. 2005 Mar; 187(5):1866-74. PMID: 15716460.

Materon IC, Beharry Z, Huang W, et al. Analysis of the context dependent sequence requirements of active site residues in the metallo-beta-lactamase IMP-1. J Mol Biol. 2004 Nov 26; 344(3):653-63. PMID: 15533435.

Zhang Z, Palzkill T. Dissecting the protein-protein interface between beta-lactamase inhibitory protein and class A beta-lactamases. J Biol Chem. 2004 Oct 08; 279(41):42860-6. PMID: 15284234.

Mustafi D, Hofer JE, Huang W, et al. Chromophoric spin-labeled beta-lactam antibiotics for ENDOR structural characterization of reaction intermediates of class A and class C beta-lactamases. Spectrochim Acta A Mol Biomol Spectrosc. 2004 May; 60(6):1279-89. PMID: 15134725.

Buynak JD, Chen H, Vogeti L, et al. Penicillin-derived inhibitors that simultaneously target both metallo- and serine-beta-lactamases. Bioorg Med Chem Lett. 2004 Mar 08; 14(5):1299-304. PMID: 14980686.

Beharry Z, Chen H, Gadhachanda VR, et al. Evaluation of penicillin-based inhibitors of the class A and B beta-lactamases from Bacillus anthracis. Biochem Biophys Res Commun. 2004 Jan 16; 313(3):541-5. PMID: 14697223.

Huang W, Beharry Z, Zhang Z, et al. A broad-spectrum peptide inhibitor of beta-lactamase identified using phage display and peptide arrays. Protein Eng. 2003 Nov; 16(11):853-60. PMID: 14631075.

Zhang Z, Palzkill T. Determinants of binding affinity and specificity for the interaction of TEM-1 and SME-1 beta-lactamase with beta-lactamase inhibitory protein. J Biol Chem. 2003 Nov 14; 278(46):45706-12. PMID: 12933802.

McKevitt M, Patel K, Smajs D, et al. Systematic cloning of Treponema pallidum open reading frames for protein expression and antigen discovery. Genome Res. 2003 Jul; 13(7):1665-74. PMID: 12805273.

Materon IC, Queenan AM, Koehler TM, et al. Biochemical characterization of beta-lactamases Bla1 and Bla2 from Bacillus anthracis. Antimicrob Agents Chemother. 2003 Jun; 47(6):2040-2. PMID: 12760895.

Majiduddin FK, Palzkill T. Amino acid sequence requirements at residues 69 and 238 for the SME-1 beta-lactamase to confer resistance to beta-lactam antibiotics. Antimicrob Agents Chemother. 2003 Mar; 47(3):1062-7. PMID: 12604542.

Majiduddin FK, Palzkill T. An analysis of why highly similar enzymes evolve differently. Genetics. 2003 Feb; 163(2):457-66. PMID: 12618385.

Rudgers GW, Palzkill T. Minimization of proteins by random fragmentation and selection. Methods Mol Biol. 2003; 230:71-81. PMID: 12824570.

Reid SD, Green NM, Sylva GL, et al. Postgenomic analysis of four novel antigens of group a streptococcus: growth phase-dependent gene transcription and human serologic response. J Bacteriol. 2002 Nov; 184(22):6316-24. PMID: 12399501.

Majiduddin FK, Materon IC, Palzkill TG. Molecular analysis of beta-lactamase structure and function. Int J Med Microbiol. 2002 Jul; 292(2):127-37. PMID: 12195735.

Palzkill T. Finding new antibiotics: the power of computational methods. Drug Discov Today. 2002 Mar 15; 7(6):347-8. PMID: 11893541.

Smajs D, McKevitt M, Wang L, et al. BAC library of T. pallidum DNA in E. coli. Genome Res. 2002 Mar; 12(3):515-22. PMID: 11875041.