Recombinant Protein Expression (Baculovirus System)
Protein Production in Insect Cells: The core can perform insect cell expression in Sf9, Sf21, and High 5 cells lines in volumes ranging from 150 ml spinner culture vessels up to culture in a 20 liter bioreactor.
Recombinant Protein Expression (E. coli)
E. coli Expression: We can construct, express and purify recombinant proteins produced in E. coli at scales ranging from small pilot tests to large scale cultures that aim to produce 100+ mg of purified protein.
Protein Purification and Chromatography Analysis
Protein Purification and Chromatography Analysis: We implement a large array of chromatography techniques to purify proteins produced in E. coli, insect and mammalian cells. We perform quality control tests and biophysical characterization of purified proteins, such as endotoxin and nucleases testing, analytical gel filtration, and Thermofluor/DSF assays.
We provide free consult with users of scientific advice on construct design in order to simplify protein purification and optimize the yield and improve the quality of protein production.
Hybridoma/Monoclonal Antibody Production
Monoclonal Antibody Production: The core can produce custom mouse monoclonal antibodies (MAbs), including those targeted to investigator designed epitopes. These services include all steps necessary, including mouse immunizations, all cell culture procedures required to construct antibody secreting hybridoma cell lines, as well as purification of the resulting MAbs and Protein Purification Services.