About the Core
The Optogenetics and Viral Design/Expression Core provides time efficient design, packaging, and distribution of viral vectors, and optogenetic manipulations at a reduced cost. Reagents and services are currently being made openly available to all investigators, with discounts and priority provided for IDDRC investigators.
A) Viral/optogenetic services to help elucidate connectivity and function neural circuits.
Services Available
Services and Reagents Offered by the Neuroconnectivity Core:
- Viral vector design and cloning
- Viral particle packaging/production
- Stereotaxic injection
- In vitro viral testing
- Slice electrophysiology
Recent Publications from the Core:
- Jiang Z, Rajamanickam S, Justice NJ. Local corticotropin-releasing factor signaling in the hypothalamic paraventricular nucleus. J Neurosci. 2018 Feb 21;38(8):1874-1890. doi: 10.1523/JNEUROSCI.1492-17.2017. Epub 2018 Jan 19. PubMed PMID: 29352046.
- McClard CK, Kochukov MY, Herman I, Liu Z, Eblimit A,..Mardon G, Arenkiel BR. POU6f1 mediates neuropeptide-dependent plasticity in the adult brain. J Neurosci. 2018 Feb 7;38(6):1443-1461. doi: 10.1523/JNEUROSCI.1641-17.2017. PMID: 29305536.
- Mangieri LR, Lu Y, Xu Y, Cassidy RM, Xu Y, Arenkiel BR, Tong Q. A neural basis for antagonistic control of feeding and compulsive behaviors. Nat Commun. 2018 Jan 4;9(1):52. doi: 10.1038/s41467-017-02534-9. PubMed PMID: 29302029.
- Huang CY, Zhang C, Zollinger DR,...Rasband MN. An αII Spectrin-Based Cytoskeleton Protects Large-Diameter Myelinated Axons from Degeneration. J Neurosci. 2017 Nov 22;37(47):11323-11334. doi: 10.1523/JNEUROSCI.2113-17.2017. PMID: 29038243
- Morikawa Y, Heallen T, Leach J, Xiao Y, Martin JF. Dystrophin-glycoprotein complex sequesters Yap to inhibit cardiomyocyte proliferation. Nature. 2017 Jul 13;547(7662):227-231. doi: 10.1038/nature22979. Epub 2017 Jun 5. PubMed PMID: 28581498
- John Lin CC, Yu K, Hatcher A, Huang TW, Lee HK, Carlson J,...Arenkiel BR, Noebels JL, Creighton CJ, Deneen B. Identification of diverse astrocyte populations and their malignant analogs. Nat Neurosci. 2017 Feb 6. PMID: 28166219.
- Quast KB, Ung K, Froudarakis E, Huang L, Herman I, Addison AP, Ortiz-Guzman J, Cordiner K, Saggau P, Tolias AS, Arenkiel BR. Developmental broadening of inhibitory sensory maps. Nat Neurosci. 2017 Feb;20(2):189-199.PMID: 28024159.
- Yang K, Broussard JI, Levine AT, Jenson D, Arenkiel BR, Dani JA. Dopamine receptor activity participates in hippocampal synaptic plasticity associated with novel object recognition. Eur J Neurosci. 2017 Jan;45(1):138-146. PMID: 27646422.
- Huang TW, Kochukov MY, Ward CS, Merritt J, Thomas K, Nguyen T, Arenkiel BR, Neul JL. Progressive changes in a distributed neural circuit underlie breathing abnormalities in mice lacking MeCP2. J Neurosci. 2016 May 18;36(20):5572-86. PMID: 27194336.
- Herman AM, Ortiz-Guzman J, Kochukov M, Herman I, Quast KB, Patel JM,...Arenkiel BR. A cholinergic basal forebrain feeding circuit modulates appetite suppression. Nature. 2016 Oct 13 ;538(7624):253-256. PMID: 27698417
Acknowledgement
We ask that users acknowledge the use of this core as follows:
"The project described was supported in part by the Neuroconnectivity Core at Baylor College of Medicine, which is supported by IDDRC Grant Number P50103555 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health & Human Development or the National Institutes of Health”.