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Center for Precision Environmental Health

DNA Sequencing Core Common Universal Primers

Master
Content

Common Universal Primers Provided by the DNA Sequencing Core

Primer

Sequence

BGH_R

5'-TAGAAGGCACAGTCGAGG

CMV

5'-GTGTACGGTGGGAGGTCT

eGFP_C

5'-ATGGTCCTGCTGGAGTTC

eGFP_N

5'-CGTCGCCGTCCAGCTCGACCAG

GL1

5'-TGTAAAACGACGGCCAGT

GL2

5'-CTTTATGTTTTTGGCGTCTTCC

hU6

5'-GAGGGCCTATTTCCCATGATT

Lambda_F

5'-GGTGGCGCAGACTCCTGGAGCC

Lambda_R

5'-GACACCAGACCAACTGGTAATG

M13F (-21)

5'-TGTAAAACGACGGCCAGT

M13R

5'-TCACACAGGAAACAGCTAT

MSCV_F

5'-CCCTTGAACCTCCTCGTTCGACC

MSCV_R

5'-GAGACGTGCTACTTCCATTTGTC

pENTR-F

5'-CTACAAACTCTTCCTGTTAGTTAG

pENTR-R

5'-ATGGCTCATAACACCCCTTG

pGEX3

5'-CCGGGAGCTGCATGTGTCAGAGG

pGEX5

5'-GGGCTGGCAAGCCACGTTTGGTG

pQE3

5'-GTTCTGAGGTCATTACTGG

pQE5

5'-CGGATAACAATTTCACACAG

RV3

5'-CTAGCAAAATAGGCTGTCCC

SP6

5'-ATTTAGGTGACACTATAG

SV40_polyA

5'-GAAATTTGTGATGCTATTGC

T3

5'-ATTAACCCTCACTAAAGGG

T7

5'-TAATACGACTCACTATAGG

T7t

5'-GCTAGTTATTGCTGAGCGG