Creation
To initiate service for creation of an adenovirus vector, we recommend you contact the Vector Lab and discuss construction of the vector, including which transfer plasmid vector to use, pShuttleX, pShuttleX-IRES-GFP, pShuttleX w/out CMV, and which Ad backbone is most appropriate for your application, pAdenoX or pAd5F35 (we do not provide aliquots of pAdenoX or pAd5F35) . Once we have a strategy set, we will send you an aliquot of the transfer plasmid. We ask that you clone your transgene into the transfer plasmid and send us 100ug of the purified plasmid DNA.
Expansion
To initiate service for expansion of an adenovirus vector, we require 1x1012 particles of purified virus. If you do not have 1x1012 particles or are not sure of the storage/production history of the vector, send us an aliquot of what you have and we will begin with a small-scale expansion. Include any information you do have about the virus including date of production, titer, particle concentration, etc.
siRNA
To initiate Ad-siRNA service we recommend you contact the Vector Lab to identify the best methodology for constructing your virus. At this stage several options are available based on promoter preference and transgene size. You will be provided with the appropriate transfer plasmid and we ask that you clone your transgene into the transfer plasmid vector. Once you have verified insertion of your gene, send us 100ug of the purified plasmid DNA.