About the Core
The protein and monoclonal antibody production core is one of the shared resources at Baylor College of Medicine main campus.
The core services including:
- Monoclonal Antibody Production
- Recombinant Protein Production (Expression and Purification)
- Protein Characterization
The core provides recombinant protein production in E.coli, insect and mammalian systems and has had more than 30 years of excellent experience in producing new mouse monoclonal antibodies using proteins, synthetic peptides and subcellular fraction as antigen.
New protein characterization services is open for users in 2022!
All core services can be requested in iLab.
Recombinant Protein Expression
The core has large refrigerated shaking incubators and bioreactors to overexpress recombinant proteins of interest in E.coli, insect and mammalian systems.
Protein and Antibody Chromatography Purification
The core implements chromatography techniques to purify protein and Mab produced in different expression systems. Quality control and biophysical characterization of purified proteins and MAbs can be performed, including endotoxin and nucleases testing, analytical gel filtration, and Thermofluor/DSF assays.
Mouse Monoclonal Antibody Production
The core can generate custom mouse monoclonal antibodies (MAbs), including those targeted to proteins, synthetic peptides and subcellular fractions.