Notification: Generation of New Monoclonal Antibodies
Generation of new monoclonal antibodies by traditional mouse hybridoma technologies is no longer being offered as a core service through the BCM Protein and Monoclonal Antibody Production Core. All other protein expression and purification services are unchanged and continue to be available including production and purification of monoclonal antibodies from existing hybridoma cell lines.
We have a formal MOU - Memorandum of Understanding with the MD Anderson Cancer Center for reciprocal access to each other’s core facilities at internal pricing. The MDACC has a monoclonal antibody core that provides comparable services to ours and they are willing and able to accept projects from BCM investigators for custom generation of new monoclonal antibodies.
Visit the MD Anderson Cancer Center website for detailed information and how to access the MDACC Core.
About the Core
The protein and monoclonal antibody production core is one of the shared resources at Baylor College of Medicine main campus.
The core services including:
- Monoclonal Antibody Production
- Recombinant Protein Production (Expression and Purification)
- Protein Characterization
The core provides recombinant protein production in E.coli, insect and mammalian systems and has had more than 30 years of excellent experience in producing new mouse monoclonal antibodies using proteins, synthetic peptides and subcellular fraction as antigen.
New protein characterization services is open for users in 2022!
All core services can be requested in iLab.
Getting Started
If you have questions and plan to make use of the core, please email atc-protein-mab@bcm.edu. We can offer consultation in Zoom or in-person meeting.
Recombinant Protein Expression
The core has large refrigerated shaking incubators and bioreactors to overexpress recombinant proteins of interest in E.coli, insect and mammalian systems.
Protein and Antibody Chromatography Purification
The core implements chromatography techniques to purify protein and Mab produced in different expression systems. Quality control and biophysical characterization of purified proteins and MAbs can be performed, including endotoxin and nucleases testing, analytical gel filtration, and Thermofluor/DSF assays.
Mouse Monoclonal Antibody Production
The core can generate custom mouse monoclonal antibodies (MAbs), including those targeted to proteins, synthetic peptides and subcellular fractions.
Protein Characterization
The core providea various protein characterization technologies and instruments. Instruments are available in the core to characterize proteins and other macromolecules.
