Recombinant Protein Production, Characterization and Crystallography Core Publications

Tsoi PS, Ferreon JC, Ferreon ACM. Initiation of hnRNPA1 Low-Complexity Domain Condensation Monitored by Dynamic Light Scattering. Int J Mol Sci. 2024 Jun 21;25(13):6825. doi: 10.3390/ijms25136825. PMID: 38999934; PMCID: PMC11241569.

Ferreon JC, Kongchan N, Tsoi PS, Choi KJ, Kenrick S, Neilson J, Ferreon ACM. Multivalent Protein-Nucleic Acid Interactions Probed by Composition-Gradient Multiangle Light Scattering. ACS Omega. 2024 Sep 21;9(39):41003-41010. doi: 10.1021/acsomega.4c06358. PMID: 39371993; PMCID: PMC11447847.

Lucas L, Nitschke L, Nguyen B, Loehr JA, Rodney GG, Cooper TA. Alternatively spliced MAP4 isoforms have key roles in maintaining microtubule organization and skeletal muscle function. iScience. 2024 Oct 5;27(11):111104. doi: 10.1016/j.isci.2024.111104. PMID: 39473976; PMCID: PMC11513531.

Irfana Saleem, Thomas Miller, Lucas Kearns, David Mitchell, Ritwika Bose, Chase A. Weidmann, Anthony M. Mustoe. Simultaneous mapping of RNA secondary, tertiary, and quaternary structure in living cells by multi-site DMS probing. bioRxiv 2024. 11.27.625770 doi: https://doi.org/10.1101/2024.11.27.625770. 

Kaundal S, Anish R, Ayyar BV, Shanker S, Kaur G, Crawford SE, Pollet J, Stossi F, Estes MK, Prasad BVV. RNA-dependent RNA polymerase of predominant human norovirus forms liquid-liquid phase condensates as viral replication factories. Sci Adv. 2024 Dec 20;10(51):eadp9333. doi: 10.1126/sciadv.adp9333. Epub 2024 Dec 20. PMID: 39705355; PMCID: PMC11661447.

Lucas L, Tsoi PS, Ferreon JC, Ferreon ACM. Tau Oligomers Resist Phase Separation. Biomolecules. 2025 Feb 26;15(3):336. doi: 10.3390/biom15030336. PMID: 40149872; PMCID: PMC11940599.

Lee J, Lai S, Yang S, Zhao S, Blanco FA, Lyons AC, Merino-Urteaga R, Ahrens JF, Nguyen NA, Liu H, Liu Z, Lambert GG, Shaner NC, Chen L, Tolias KF, Zhang J, Ha T, St-Pierre F. Bright and photostable yellow fluorescent proteins for extended imaging. Nat Commun. 2025 Apr 4;16(1):3241. doi: 10.1038/s41467-025-58223-5. PMID: 40185748; PMCID: PMC11971446.

Mann MD, Wang M, Ferreon JC, Tsoi PS, Suess MP, Jain A, Malovannaya A, Alvarez RV, Pascal BD, Kumar R, Edwards DP, Griffin PR. Structural proteomics defines a sequential priming mechanism for the progesterone receptor. Nat Commun. 2025 May 12;16(1):4403. doi: 10.1038/s41467-025-59458-y. PMID: 40355435; PMCID: PMC12069617.

Wu H, Sun L, Huo T, Wensel TG, Horrigan FT, Wang Z. The identification of XPR1 as a voltage- and phosphate-activated phosphate-permeable ion channel. Nat Commun. 2025 May 15;16(1):4519. doi: 10.1038/s41467-025-59678-2. PMID: 40374661; PMCID: PMC12081713.

Mitchell D, Cotter J, Saleem I, Mustoe AM. Mutation signature filtering enables high-fidelity RNA structure probing at all four nucleobases with DMS. Nucleic Acids Res. 2023 Sep 8;51(16):8744-8757. doi: 10.1093/nar/gkad522. PMID: 37334863; PMCID: PMC10484685.

Salmen W, Hu L, Bok M, Chaimongkol N, Ettayebi K, Sosnovtsev SV, Soni K, Ayyar BV, Shanker S, Neill FH, Sankaran B, Atmar RL, Estes MK, Green KY, Parreño V, Prasad BVV. A single nanobody neutralizes multiple epochally evolving human noroviruses by modulating capsid plasticity. Nat Commun. 2023 Oct 16;14(1):6516. doi: 10.1038/s41467-023-42146-0. PMID: 37845211; PMCID: PMC10579229.

Goike J, Hsieh CL, Horton AP, Gardner EC, Zhou L, Bartzoka F, Wang N, Javanmardi K, Herbert A, Abbassi S, Xie X, Xia H, Shi PY, Renberg R, Segall-Shapiro TH, Terrace CI, Wu W, Shroff R, Byrom M, Ellington AD, Marcotte EM, Musser JM, Kuchipudi SV, Kapur V, Georgiou G, Weaver SC, Dye JM, Boutz DR, McLellan JS, Gollihar JD. SARS-COV-2 Omicron variants conformationally escape a rare quaternary antibody binding mode. Commun Biol. 2023 Dec 11;6(1):1250. doi: 10.1038/s42003-023-05649-6. PMID: 38082099; PMCID: PMC10713552.

Liu Z, Smith H, Criglar JM, Valentin AJ, Karandikar U, Zeng XL, Estes MK, Crawford SE. Rotavirus-mediated DGAT1 degradation: A pathophysiological mechanism of viral-induced malabsorptive diarrhea. Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2302161120. doi: 10.1073/pnas.2302161120. Epub 2023 Dec 11. PMID: 38079544; PMCID: PMC10743370.

Lee SH, Suh JH, Heo MJ, Choi JM, Yang Y, Jung HJ, Gao Z, Yu Y, Jung SY, Kolonin MG, Cox AR, Hartig SM, Eltzschig HK, Ju C, Moore DD, Kim KH. The Hepatokine Orosomucoid 2 Mediates Beneficial Metabolic Effects of Bile Acids. Diabetes. 2024 May 1;73(5):701-712. doi: 10.2337/db23-0520. PMID: 38320268; PMCID: PMC11043061.

Gu T, Hao D, Woo J, Huang TW, Guo L, Lin X, Guzman AG, Tovy A, Rosas C, Jeong M, Zhou Y, Deneen B, Huang Y, Li W, Goodell MA. The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development. Nat Genet. 2022 May;54(5):625-636. doi: 10.1038/s41588-022-01063-6. Epub 2022 May 9. PMID: 35534561; PMCID: PMC9295050.

Versteeg L, Adhikari R, Poveda C, Villar-Mondragon MJ, Jones KM, Hotez PJ, Bottazzi ME, Tijhaar E, Pollet J. Location and expression kinetics of Tc24 in different life stages of Trypanosoma cruzi. PLoS Negl Trop Dis. 2021 Sep 3;15(9):e0009689. doi: 10.1371/journal.pntd.0009689. PMID: 34478444; PMCID: PMC8415617. 

Alvarado G, Salmen W, Ettayebi K, Hu L, Sankaran B, Estes MK, Venkataram Prasad BV, Crowe JE Jr. Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses. Nat Commun. 2021 Jul 4;12(1):4320. doi: 10.1038/s41467-021-24649-w. Erratum in: Nat Commun. 2021 Oct 14;12(1):6090. PMID: 34262046; PMCID: PMC8280134.

Strutzenberg TS, Zhu Y, Novick SJ, Garcia-Ordonez RD, Doebelin C, He Y, Chang MR, Kamenecka TM, Edwards DP, Griffin PR. Conformational Changes of RORγ During Response Element Recognition and Coregulator Engagement. J Mol Biol. 2021 Nov 5;433(22):167258. doi: 10.1016/j.jmb.2021.167258. Epub 2021 Sep 20. PMID: 34547329; PMCID: PMC8556364.

Kumar D, Yu X, Crawford SE, Moreno R, Jakana J, Sankaran B, Anish R, Kaundal S, Hu L, Estes MK, Wang Z, Prasad BVV. 2.7 Å cryo-EM structure of rotavirus core protein VP3, a unique capping machine with a helicase activity. Sci Adv. 2020 Apr 15;6(16):eaay6410. doi: 10.1126/sciadv.aay6410. PMID: 32494598; PMCID: PMC7159914.

Yu X, Yi P, Hamilton RA, Shen H, Chen M, Foulds CE, Mancini MA, Ludtke SJ, Wang Z, O'Malley BW. Structural Insights of Transcriptionally Active, Full-Length Androgen Receptor Coactivator Complexes. Mol Cell, 2020 Sep 3;79(5):812-823. doi: 10.1016/j.molcel. 2020.06.031. Epub 2020 Jul 14. PMID: 32668201; PMCID: PMC7483370.

Bimler L, Ronzulli SL, Song AY, Johnson SK, Jones CA, Kim T, Le DT, Tompkins SM, Paust S. Matrix Protein 2 Extracellular Domain-Specific Monoclonal Antibodies Are an Effective and Potentially Universal Treatment for Influenza A. J Virol. 2020 Dec 2:JVI.01027-20. doi: 10.1128/JVI.01027-20. Epub ahead of print. Erratum in: J Virol. 2022 Oct 12;96(19):e0135822. PMID: 33268521; PMCID: PMC8092830.

Chen S, Jiao L, Liu X, Yang X, Liu X. A Dimeric Structural Scaffold for PRC2-PCL Targeting to CpG Island Chromatin. Mol Cell. 2020 Mar 19;77(6):1265-1278. doi: 10.1016/j.molcel. 2019.12.019. Epub 2020 Jan 17. PMID: 31959557; PMCID: PMC7571800.

Fuery A, Pursell T, Tan J, Peng R, Burbelo PD, Hayward GS, Ling PD. Lethal Hemorrhagic Disease and Clinical Illness Associated with Elephant Endotheliotropic Herpesvirus 1 Are Caused by Primary Infection: Implications for the Detection of Diagnostic Proteins. J Virol. 2020 Jan 17;94(3):e01528-19. doi: 10.1128/JVI.01528-19. PMID: 31723022; PMCID: PMC7000966.

Lee ME, Dou X, Zhu Y, Phillips KJ. Refolding Proteins from Inclusion Bodies using Differential Scanning Fluorimetry Guided (DGR) Protein Refolding and MeltTraceur Web. Curr Protoc Mol Biol. 2019 Jan;125(1):e78. doi: 10.1002/cpmb.78. Epub 2018 Oct 29. PMID: 30371021.

Vossaert L, Wang Q, Salman R, McCombs AK, Patel V, Qu C, Mancini MA, Edwards DP, Malovannaya A, Liu P, Shaw CA, Levy B, Wapner RJ, Bi W, Breman AM, Van den Veyver IB, Beaudet AL. Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis. Am J Hum Genet. 2019 Dec 5;105(6):1262-1273. doi: 10.1016/j.ajhg.2019.11.004. Epub 2019 Nov 27. PMID: 31785788; PMCID: PMC6904821.