Wen-Hsiang Chen, Ph.D.
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Wen-Hsiang Chen, Ph.D.
Assistant Professor
Positions
- Assistant Professor
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Pediatrics-Tropical Medicine
Baylor College of Medicine
Houston, Texas
- Director of Quality Control and Analytical Development
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Texas Children's Hospital Center for Vaccine Development
Addresses
- 1102 BATES AVE, STE 550, FEIGIN CENTER (Lab)
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HOUSTON, TX 77030
United States
Education
- BS from National Taiwan University
- Taipei, Taiwan
- Chemical Engineering
- PhD from University of Houston
- Houston, Texas United States
- Chemical Engineering
Professional Interests
- Vaccine
- Quality Control
- Gene cloning and expressing
- Biotechnology
- Protein purification
- Upstream Process-Fermentation
- Analytical Development
Websites
Selected Publications
- S Dhamane, F Ruiz-Ruiz, WH Chen, K Kourentzi, J Benavides, M Rito-Palomares, RC Willson. "Spermine Sepharose as a Clustered-Charge Anion Exchange Adsorbent.." J Chromatogr A. 2014 Jan;
- L Kisley, J Chen, AP Mansur, B Shuang, K Kourentzi, MV Poongavanam, WH Chen, S Dhamane, RC Willson, CF Landes "Unified super-resolution experiments and stochastic theory provide mechanistic insight into protein ion-exchange adsorptive separations.." PNAS. 2014 Jan;
- WH Chen+, L Du+, SM Chag, C Ma, N Tricoche, X Tao, CA Seid, EM Hudspeth, S Lustigman, CK Tseng, ME Bottazzi, PJ Hotez, B Zhan, S Jiang. "Yeast-expressed recombinant protein of the receptor-binding domain in SARS-CoV spike protein with deglycosylated forms as a SARS vaccine candidate.." Hum Vaccin Immunother. 2013 Dec;
- WH Chen, MP Nyon, MV Poongavanam, Z Liu, AB Biter, RT Kundu, U Strych, PJ Hotez, ME Bottazzi "Process Characterization and Biophysical Analysis for a Yeast-expressed Phlebotomus papatasi Salivary Protein (PpSP15) as a Leishmania vaccine candidate." J Pharm Sci.. 2020 Feb 15; Pubmed PMID: 32070701
- WH Chen, U Strych, PJ Hotez, ME Bottazzi "The SARS-CoV-2 Vaccine Pipeline: an Overview." Curr. Trop. Med. Rep.. 2020 Mar 3;
- Marcinkiewicz AL, Lieknina I, Yang X, Lederman PL, Hart TM, Yates J, Chen WH, Bottazzi ME, Mantis NJ, Kraiczy P, Pal U, Tars K, Lin YP. "The Factor H-binding site of CspZ as a protective target against multi-strain, tick-transmitted Lyme disease." Infection and immunity. 2020 Mar; Pubmed PMID: 32122944
- AB Biter, J Pollet, WH Chen, U Strych, PJ Hotez, ME Bottazzi "A method to probe protein structure from UV absorbance spectra." Anal Biochem. 2019 Dec 15; Pubmed PMID: 31550438
- I Kanakaraj, WH Chen, M Poongavanam, S Dhamane, LJ Stagg, JE Ladbury, K Kourentzi, U Strych, RC Willson. "Biophysical characterization of VEGF-aHt DNA aptamer interactions.." Int J Biol Macromol. 2013 Mar;
- AB Biter, S Weltje, EM Hudspeth, CA Seid, CP McAtee, WH Chen, JB Pollet, U Strych, PJ Hotez, ME Bottazzi "Characterization and Stability of Trypanosoma cruzi 24-C4 (Tc24-C4), a Candidate Antigen for a Therapeutic Vaccine Against Chagas Disease." J Pharm Sci. 2018 May;107(5):1468-1473. Pubmed PMID: 29274820
- M Adhikari, S Dhamane, AEV Hagström, G Garvey, WH Chen, K Kourentzi, U Strych, RC Willson. "Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays.." Analyst. 2013 Jul;
- CR Daniels, L Kisley, H Kim, WH Chen, MV Poongavanam, C Reznik, K Kourentzi, RC Willson, CF Landes. "Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents." J Mol Recognit. 2012 Aug;
- WH Chen, JY Fu, K Kourentzi, RC Willson "Nucleic acid affinity of clustered-charge anion exchange adsorbents: effects of ionic strength and ligand density." J Chromatogr A. 2011 Jan;
- WH Chen, SM Chag, MV Poongavanam, AB Biter, EA Ewere, W Rezende, CA Seid, EM Hudspeth, J Pollet, CP McAtee, U Strych, ME Bottazzi, PJ Hotez "Optimization of the Production Process and Characterization of the Yeast-Expressed SARS-CoV Recombinant Receptor-Binding Domain (RBD219-N1), a SARS Vaccine Candidate." J Pharm Sci. 2017 Apr; Pubmed PMID: 28456726
- CR Daniels, C Reznik, R Kilmer, MJ Felipe, MC Tria, K Kourentzi, WH Chen, RC Advincula, RC Willson, CF Landes "Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy." Colloids Surf B Biointerfaces. 2011 Nov;
- WH Chen, PJ Hotez, ME Bottazzi "Potential for Developing a SARS-CoV Receptor-Binding Domain (RBD) Recombinant Protein as a Heterologous Human Vaccine Against Coronavirus Infectious Disease (COVID)-19." Hum Vaccin Immunother. 2020 Apr 16; Pubmed PMID: 32298218
- WH Chen, X Tao, AS Agrawal, A Algaissi, BH Peng, J Pollet, U Strych, ME Bottazzi, PJ Hotez, S Lustigman, L Du, S Jiang, CK Tseng "Yeast-Expressed SARS-CoV Recombinant Receptor-Binding Domain (RBD219-N1) Formulated with Aluminum Hydroxide Induces Protective Immunity and Reduces Immune Enhancement." Vaccine. 2020 Sep; Pubmed PMID: 33039209
- Z Liu, R Kundu, S Damena, AB Biter, MP Nyon, W-H Chen, B Zhan, U Strych, PJ. Hotez, ME Bottazzi "A scalable and reproducible manufacturing process for Phlebotomus papatasi salivary protein PpSP15, a vaccine candidate for leishmaniasis." Protein Expr Purif. 2020 Sep; Pubmed PMID: 32920041
- Pino M, et al "A yeast expressed RBD-based SARS-CoV-2 vaccine formulated with 3M-052-alum adjuvant promotes protective efficacy in non-human primates." Sci Immunol. 2021; Pubmed PMID: 34266981
- Lee J, Liu Z, Chen WH, Wei J, Kundu R, Adhikari R, Rivera JA, Gillespie PM, Strych U, Zhan B, Hotez PJ, Bottazzi ME "Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain-based vaccine candidate, RBD219-N1C1." Appl Microbiol Biotechnol.. 2021; Pubmed PMID: 33959781
- Pollet J, Chen WH, Versteeg L, Keegan B, Zhan B, Wei J, Liu Z, Lee J, Kundu R, Adhikari R, Poveda C, Villar MJ, de Araujo Leao AC, Altieri Rivera J, Momin Z, Gillespie PM, Kimata JT, Strych U, Hotez PJ, Bottazzi ME "SARS-CoV-2 RBD219-N1C1: A yeast-expressed SARS-CoV-2 recombinant receptor-binding domain candidate vaccine stimulates virus neutralizing antibodies and T-cell immunity in mice." Hum Vaccin Immunother. 2021; Pubmed PMID: 33847226
- Chen WH, Pollet J, Strych U, Lee J, Liu Z, Kundu RT, Versteeg L, Villar MJ, Adhikari R, Wei J, Poveda C, Keegan B, Bailey AO, Chen YL, Gillespie PM, Kimata JT, Zhan B, Hotez PJ, Bottazzi ME "Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate.." Protein Expr Purif. 2021; Pubmed PMID: 34688919
- Chen WH, Wei J, Kundu RT, Adhikari R, Liu Z, Lee J, Versteeg L, Poveda C, Keegan B, Villar MJ, de Araujo Leao AC, Rivera JA, Gillespie PM, Pollet J, Strych U, Zhan B, Hotez PJ, Bottazzi ME "Genetic modification to design a stable yeast-expressed recombinant SARS-CoV-2 receptor binding domain as a COVID-19 vaccine candidate." Biochim Biophys Acta Gen Subj. 2021; Pubmed PMID: 33731300
- Pollet J*, Chen WH*, Strych U* (equal contribution) "Recombinant protein vaccines, a proven approach against coronavirus pandemics." Adv Drug Deliv Rev. 2021; Pubmed PMID: 33421475
Memberships
- American Society of Microbiology
- Member
- American Chemical Society
- Former Member
Intellectual Property
- A novel SARS immunogenic composition
- Product Patent #US20160376321A1 (Approved)
- Co Inventors: PJ Hotez, ME Bottazzi, B Zhan, W-H Chen,SM Chag
Languages
Manderin Chinese, Taiwanese
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