Positions
- Associate Professor
-
Biochemistry-Tsai Lab
Baylor College of Medicine
Houston, TX US
- Managing Director
-
X-ray Crystallography Facility
Baylor College of Medicine
Houston, Texas United States
- Member
-
Dan L Duncan Comprehensive Cancer Center
Baylor College of Medicine
Houston, Texas United States
Addresses
- Baylor College of Medicine (Office)
-
BCM-Cullen Building
Room: BCMC-371A1
Houston, TX 77030-3498
United States
(713) 798-4390
slee@bcm.edu
Education
- Post-Doctoral Fellowship at Yale University
- 10/2000 - New Haven, Connecticut United States
- Molecular Biophysics and Biochemistry
- Post-Doctoral Fellowship at Purdue University
- 03/1998 - West Lafayette, Indiana United States
- Biological Sciences
- PhD from Purdue University
- 08/1997 - West Lafayette, Indiana United States
- Biological Sciences
- MS from Seoul National University
- 02/1991 - Seoul, Korea
- Chemistry
- BS from Seoul National University
- 02/1989 - Seoul, Korea
- Chemistry
Honors & Awards
- Beginning Grant-in-Aid Award
- American Heart Association Texas Affiliate
- New Investigator Award
- Department of Defense
- International Union of Crystallography Young Scientist Travel Grant
- International Union of Crystallography
- Korean Government Overseas Scholarship
- Korean Government
- Frederick N. Andrews Doctoral Fellowship
- Purdue University
- Undergraduate Scholarship
- Korea Scholarship Foundation for Future Leaders
Professional Interests
- Antibiotic Resistance
- Cryo-electron Microscopy and Tomography
- Membranes and Membrane Proteins
- Nano Medicine and Drug Design
- Protein Structure Function and Protein Folding
- Structural Biology and Macromolecular Assemblies by Organism
- Bacteria and Phage
- Human
- Yeast and Dicty
Professional Statement
Mitochondrial Proteostasis MaintenanceThe broad and long-term research objective of my laboratory is to provide a molecular understanding of the protein quality control machinery that maintains mitochondrial proteostasis. On-going research focuses on determining the structure, function, and mechanism of molecular chaperones, membrane-associated proteases, kinases and enzymes using a multi-pronged approach combining X-ray crystallography and cryoEM/cryoET with biochemical, biophysical, and functional studies. The goal of our research is to provide a detailed mechanistic understanding of mitochondrial quality control proteins in order to exploit this information for rationale structure-based drug development.
Websites
Selected Publications
- Lee, J., Kim, J.-H., Biter, A.B., Sielaff, B., Lee, S.* and Tsai, F.T.F "Heat shock protein (Hsp) 70 is an activator of the Hsp104 motor." Proc Natl Acad Sci. 2013 May 21; 110 : 8513-8518. Pubmed PMID: 23650362
- Biter, A.B., Lee, S., Sung, N. and Tsai, F.T.F "Structural basis for inter-subunit signaling in a protein disaggregating machine." Proc Natl Acad Sci. 2012 July 31; 109 (31): 12515-20. Pubmed PMID: 22802670
- Biter, A.B., Lee, J., Sung, N., Tsai, F.T.F. and Lee, S "Functional analysis of conserved cis- and trans-elements in the Hsp104 protein disaggregating machine." J. Structural Biol. 2012 August ; 179 (2): 172-80. Pubmed PMID: 22634726
- Lee, S.*, Augustin, S., Tatsuta, T., Gerdes, F., Langer, T. and Tsai, F.T.F "Electron cryomicroscopy structure of a membrane-anchored mitochondrial AAA protease." J. Biol. Chem. 2011 February 11; 286 (6): 4404-4411. Pubmed PMID: 21147776
- Lee, S., Sielaff, B., Lee, J. and Tsai, F.T.F "CryoEM structure of Hsp104 and its mechanistic implication for protein disaggregation." Proc. Natl. Acad. Sci. 2010 May 4; 107 : 8135-8140. Pubmed PMID: 20404203
- Augustin, S., Gerdes, F., Lee, S., Tsai, F.T.F., Langer, T. and Tatsuta, T "An intersubunit signaling network coordinates ATP hydrolysis by m-AAA proteases." Mol. Cell. 2009 September 11; 35 (5): 574-85. Pubmed PMID: 19748354
- Lee, S., Choi, J.-M. and Tsai, F.T.F "Visualizing the ATPase cycle in a protein disaggregating machine: structural basis for substrate binding by ClpB." Mol. Cell. 2007 January 26; 25 (2): 261-71. Pubmed PMID: 17244533
- Haslberger, T., Weibezahn, J., Zahn, R., Lee, S., Tsai, F.T.F., Bukau, B. and Mogk, A "M domains couple the ClpB threading motor with the DnaK chaperone activity." Mol Cell. 2007 January 26; 25 (2): 247-60. Pubmed PMID: 17244532
- Rees, I., Lee, S.*, Kim, H. and Tsai, F.T.F "The E3 ubiquitin ligase CHIP binds the androgen receptor in a phosphorylation-dependent manner." Biochim. Biophys. Acta. 2006 June ; 1764 (6): 1073-1079. Pubmed PMID: 16725394
- Weibezahn, J., Tessarz, P., Schlieker, C., Zahn, R., Maglica, Z., Lee, S., Zentgraf, H., Weber-Ban, E., Dougan, D., Tsai, F.T.F., Mogk, A. and Bukau, B "Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB." Cell. 2004 November 24; 119 (5): 653-65. Pubmed PMID: 15550247
Memberships
- American Crystallography Association
- Member
- American Association for the Advancement of Science
- Member
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