Our services provide customized MHC/peptide tetramers for identification of antigen specific T lymphocytes by flow cytometry. Tetramers and/or their derivatives are provided for research purposes only. Their use for commercial purposes is prohibited.
Choices of fluorescent labels: R-phycoerythrin (PE) or allophycocyanin (APC).
Standard product size: 100μg of MHC/peptide complex protein, which is sufficient for 400 tests (PE labeled) or 250 tests (APC labeled).
Other product size: We may split standard vials into smaller aliquots upon request, aliquot size can be as low as 25μg each.
Monomers: Monomeric soluble MHC/peptide complex is also available upon request.
Clinical Research Grade Reagents: We provide Class I tetramers for certain clinical researches.
- CD8 binding-deficient MHC I tetramers: Containing mutations in the MHC α3 domain that ablate CD8 binding; Can be used to quantitate and to sort CD8-independent T cells.
- Chimeric Tetramer: Containing MHC Human/Mouse chimeric heavy-chain for binding affinity studies.
Available MHC Alleles
Please refer to Table 1 for the current available MHC alleles.
If the requested MHC allele is not in the table or if there is other requirements, such as a specific b2-microglobulin is needed to reconstitute class I of an unusual species, the requestor must supply the cDNA. We will perform the subcloning to the appropriate vector for protein expression and additional charges may apply.
All expression plasmids containing MHC alleles will be retained at our facility and will be used for future production and distribution of tetramer reagents.
Requestors incur the cost of peptide that is needed in the tetramer production. You can either provide your own peptide or order from us with an additional cost. If provide your own peptide, please send 10mg of it to us. HPLC chromatograph and mass spectrophotometric analysis for the peptide will also be required.