Tom Cooper Lab

Master
Media Component
Tom Cooper Lab Members 2021
Content

Group photo of Tom Cooper, M.D., and his lab members. 

Heading

About the Lab

Media Component
Research at The Cooper Lab.
Content

The realization that most human genes generate multiple mRNAs encoding divergent protein isoforms via alternative splicing and polyadenylation has revealed extensive regulation that remains to be explored.

Alternative pathways of RNA processing are regulated in response to signaling cues often coordinating expression of gene networks in response to physiological change including in development and disease.

We are interested in understanding the mechanisms and consequences of this regulation, from how RNA binding proteins and signaling pathways coordinate RNA processing networks to the functional consequences of the different protein isoforms that are expressed in different cell states.

We also investigate the pathogenic mechanisms of myotonic dystrophy, type 1 (DM1), an autosomal dominant neuromuscular disorder affecting multiple tissues including muscle, heart and the central nervous system. The pathogenic mechanism is disruption of developmentally regulated RNA processing, primarily alternative splicing, in which failure to express adult splicing patterns causes primary features of the disease.

The understanding of the molecular mechanisms of DM1 pathogenesis has led to development of several therapeutic approaches some of which are being testing using mouse models established in the lab.

These investigations utilize a combination of cell culture and genetic models including transgenic and knock out mouse lines for RNA binding proteins, CRISPR-derived mouse lines in which specific alternative exons are removed, and DM1 mouse models.

The overlapping areas of investigation in the lab lead to synergistic and collaborative interactions in which knowledge gained in one area fosters progress in the others.

Heading

Cooper Lab News

Content

Dr. Cooper was awarded the Steinert Award for Excellence in Myotonic Dystrophy Basic Science Research at the 13th International Myotonic Dystrophy Consortium Meeting held in Osaka, Japan.

Dr. Thomas Cooper received the Diana Brown Memorial BRASS Mentor of the year award, nominated by the BRASS students.

Larissa Nitschke's abstract was selected for a talk at the 2022 Post-Transcriptional Gene Regulation Gordon Research Seminar July 2022 that will be held the day before the associated Gordon Research Conference

Larissa Nitschke's abstract was selected for an oral presentation at the 13th International Myotonic Dystrophy Consortium Meeting held in Osaka, Japan June 2022

Janel Peterson won the best poster award for basic research at the Department of Pathology & Immunology Baylor College of Medicine Trainee Research Symposium

Larissa Nitschke has been awarded a two-year postdoctoral fellowship award from the Myotonic Dystrophy Foundation beginning in 2022.

View all Lab News
Heading

Reagents

Content

The following plasmids are available through Addgene.

FRE5 (FLAGNLSREDGFP5)
FRE5Cf (FLAGNLSREDGFP5Claf)
RG6
RHCglo
RTB300
R300TA
DT960
DT480
DT240
DT40
DT12A
DT0
pBItetDT960GFP
pBItetDT12nGFP
FLYLQ (Flag-CELF1)
pBItetDMPKSGFP
pBItetDT240GFP
pBItetDT480GFP
FlagETR3 (CELF2)
FlagMBNL1-41
FlagMBNL3