About Us

Gene Vector Core facility is part of Baylor College of Medicine Advanced Technology Laboratories. 

Gene Vector Core

The Gene Vector Core was founded in 1997 as the Adenovirus Vector Production Core which specialized in the production of helper-dependent adenoviral vector (HDAd). Over the years, we have extended our expertise to other popular viral vector systems. Our core has been one of BCM Advanced Technology Cores since October 2013 when it transitioned into the DNA Sequencing and Gene Vector Core. The service is open to all domestic and international institutions.

Services

We are currently producing:

  • First-generation adenovirus (FGAd)
  • Helper-dependent adenoviral vector (HDAd, serotype 5, 5/35-fiber, and custom targeted)
  • Adeno-associated viral vector (AAV, serotype 1, 2, 5, 6, 8, 9, DJ and DJ8)
  • VSVG-pseudotyped lentiviral vector (LV) with second-, third-, and fourth-generation packaging systems and 2nd and 3rd generation non integrating LV
  • Retroviral vector (RV), ecotropic and amphotropic
  • Subcloning and plasmid preparations for viral vector production

Viral vectors are produced and purified by basic protocols (e.g., centrifugation by density gradient). Infectious titer of viral vectors is subject to an additional charge. The core has various expression cassettes and basic transfer vectors for expression of your genes including shRNA which are provided to our customers for free of charge. There is no additional charge for packaging plasmids and helper virus. There is no single viral vector system fit for all purposes. The core provides consultations for which vector systems meet the needs of individual investigators and facilitates their research via viral packaging service.

Fees

Viral vectors are produced and purified by basic protocols (e.g., centrifugation by density gradient). Infectious titer of viral vectors is subject to an additional charge. The core has developed various expression cassettes and basic transfer vectors for expression of your genes, which has been deposited to Addgene. There is no single viral vector system fit for all purposes. The core provides consultations for which vector systems meet the needs of individual investigators and facilitates their research via viral packaging service.