Lisa Bouchier-Hayes, Ph.D.

Assistant Professor

(832) 824-3320

Email

bouchier@bcm.edu

Positions

Assistant Professor: Pediatrics
Hem-Onc Cell & Gene Therapy
Baylor College of Medicine
Houston, TX, US

Assistant Professor: Molecular and Cellular Biology
Baylor College of Medicine
Houston, Texas, United States

Associate Professor: Pediatrics
Hematology-Oncology
Baylor College of Medicine
HOUSTON, Texas, United States

Staff Scientist: Immunology
St Jude Children's Research Hospital
Memphis, Tennessee, United States

Addresses

Texas Children's Hospital (Office): 1102 Bates Ave
Feigin Tower Suite 1030.08
Houston, TX, 77030
United States
bouchier@bcm.edu

Education

BA from Trinity College Dublin: 06/1998 - Dublin, Ireland

PhD from Trinity College Dublin: 06/2002 - Dublin, Ireland

Postdoctoral Fellowship at LaJolla Institute of Allergy and Immunology: 01/2006 - San Diego, California, United States; Molecular mechanisms of apoptosis

Postdoctoral Fellowship at St Jude Children's Research Hospital: 01/2011 - Memphis, Tennessee, United States; Molecular mechanisms of apoptosis

Professional Statement

The focus of my lab is to determine how members of the caspase family of proteases function in apoptosis and non-apoptotic processes to protect from disease. Specifically, we develop and use cutting edge live cell imaging-based methodologies to interrogate activation of distinct caspases in normal and transformed cells. Our research program is currently focused on two major areas: (1) caspase regulation in cancer; and (2) caspase activation during inflammation. The rationale for these investigations is that caspases protect from cancer and other diseases, by engaging diverse cellular mechanisms in addition to cell death, such as cell division, inflammation, and DNA repair. The ultimate goal is to understand how these caspase pathways can be manipulated for preventative and therapeutic purposes.

Websites

VIICTR Publications List

Bouchier-Hayes Lab Website

Selected Publications

Beatriz E Bolívar, Alexandra N Brown-Suedel, Brittany A Rohrman, Chloé I Charendoff, Vanda Yazdani, John D Belcher , Gregory M Vercellotti , Jonathan M Flanagan, Lisa Bouchier-Hayes. " Noncanonical Roles of Caspase-4 and Caspase-5 in Heme-Driven IL-1β Release and Cell Death " J Immunol .. 2021 Apr 15; 206 (8) : 1878-1889.
Pubmed PMID: 33741688.
Ando K, Parsons MJ, Shah RB, Charendoff CI, Paris SL, Liu PH, Fassio SR, Rohrman BA, Thompson R, Oberst A, Sidi S, Bouchier-Hayes L.. " NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus. " J Cell Biol.. 2017 ; 216 : 1795-1810.
Pubmed PMID: 28432080.
Sanders MG, Parsons MJ, Howard AG, Liu J, Fassio SR, Martinez JA, Bouchier-Hayes L. " Single-cell imaging of inflammatory caspase dimerization reveals differential recruitment to inflammasomes. " Cell Death Dis. 2015 Jul 9; 6 : e1813.
Pubmed PMID: 26158519.
Ando M, Hoyos V, Yagyu S, Tao W, Ramos CA, Dotti G, Brenner MK, Bouchier-Hayes L.. " Bortezomib sensitizes non-small cell lung cancer to mesenchymal stromal cell-delivered inducible caspase-9-mediated cytotoxicity. " Cancer Gene Ther. 2014 Nov ; 21 (11) : 472-82.
Pubmed PMID: 25323693.
Parsons MJ, McCormick L, Janke L, Howard A, Bouchier-Hayes L, Green DR. " Genetic deletion of caspase-2 accelerates MMTV/c-neu-driven mammary carcinogenesis in mice. " Cell Death Differ.. 2013 Sep ; 20 (9) : 1174-82.
Pubmed PMID: 23645210.
Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SW, Dillon CP, Flanagan JM, Beere HM, Green DR. " Characterization of cytoplasmic caspase-2 activation by induced proximity. " Mol. Cell. 2009 Sep 24; 35 (6) : 830-40.
Pubmed PMID: 19782032.
Bouchier-Hayes L, McBride S, van Geelen CM, Nance S, Lewis LK, Pinkoski MJ, Beere HM. " Fas ligand gene expression is directly regulated by stress-inducible heat shock transcription factor-1. " Cell Death Differ.. 2010 Jun ; 17 (6) : 1034-46.
Pubmed PMID: 20150914.
Bouchier-Hayes L, Conroy H, Egan H, Adrain C, Creagh EM, MacFarlane M, Martin SJ. " CARDINAL, a novel caspase recruitment domain protein, is an inhibitor of multiple NF-kappa B activation pathways. " J. Biol. Chem.. 2001 Nov 23; 276 (47) : 44069-77.
Pubmed PMID: 11551959.
Dawar S, Lim Y, Puccini J, White M, Thomas P, Bouchier-Hayes L, Green DR, Dorstyn L, Kumar S.. " Caspase-2-mediated cell death is required for deleting aneuploid cells. " Oncogene. 2016 Dec 19;
Pubmed PMID: 27991927.
Manton CA, Johnson B, Singh M, Bailey CP, Bouchier-Hayes L, Chandra J. " Induction of cell death by the novel proteasome inhibitor marizomib in glioblastoma in vitro and in vivo. " Sci Rep. 2016 Jan 25; 6 : 18953.
Pubmed PMID: 26804704.
Ichim G, Lopez J, Ahmed SU, Muthalagu N, Giampazolias E, Delgado ME, Haller M, Riley JS, Mason SM, Athineos D, Parsons MJ, van de Kooij B, Bouchier-Hayes L, Chalmers AJ, Rooswinkel RW, Oberst A, Blyth K, Rehm M, Murphy DJ, Tait SW.. " Limited mitochondrial permeabilization causes DNA damage and genomic instability in the absence of cell death. " Mol Cell.. 2015 Mar 5; 57 (5) : 860-72.
Pubmed PMID: 25702873.
Ando K, Kernan JL, Liu PH, Sanda T, Logette E, Tschopp J, Look AT, Wang J, Bouchier-Hayes L, Sidi S. " PIDD Death-Domain Phosphorylation by ATM Controls Prodeath versus Prosurvival PIDDosome Signaling. " Mol. Cell. 2012 Sep 14; 47 (5) : 681-93.
Pubmed PMID: 22854598.
Llambi F, Moldoveanu T, Tait SW, Bouchier-Hayes L, Temirov J, McCormick LL, Dillon CP, Green DR. " A Unified Model of Mammalian BCL-2 Protein Family Interactions at the Mitochondria. " Mol. Cell. 2011 Nov 18; 44 (4) : 517-31.
Pubmed PMID: 22036586.
Tait SW, Parsons MJ, Llambi F, Bouchier-Hayes L, Connell S, Muñoz-Pinedo C, Green DR. " Resistance to caspase-independent cell death requires persistence of intact mitochondria. " Dev. Cell. 2010 May 18; 18 (5) : 802-13.
Pubmed PMID: 20493813.
Colell A, Ricci JE, Tait S, Milasta S, Maurer U, Bouchier-Hayes L, Fitzgerald P, Guio-Carrion A, Waterhouse NJ, Li CW, Mari B, Barbry P, Newmeyer DD, Beere HM, Green DR. " GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation. " Cell. 2007 Jun 1; 129 (5) : 983-97.
Pubmed PMID: 17540177.
Bonzon C, Bouchier-Hayes L, Pagliari LJ, Green DR, Newmeyer DD. " Caspase-2-induced apoptosis requires bid cleavage: a physiological role for bid in heat shock-induced death. " Mol. Biol. Cell. 2006 May ; 17 (5) : 2150-7.
Pubmed PMID: 16495337.
Chipuk JE, Bouchier-Hayes L, Kuwana T, Newmeyer DD, Green DR. " PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. " Science. 2005 Sep 9; 309 (5741) : 1732-5.
Pubmed PMID: 16151013.
Kuwana T, Bouchier-Hayes L, Chipuk JE, Bonzon C, Sullivan BA, Green DR, Newmeyer DD. " BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly. " Mol. Cell. 2005 Feb 18; 17 (4) : 525-35.
Pubmed PMID: 15721256.
Chipuk JE, Kuwana T, Bouchier-Hayes L, Droin NM, Newmeyer DD, Schuler M, Green DR. " Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis. " Science. 2004 Feb 13; 303 (5660) : 1010-4.
Pubmed PMID: 14963330.
Hoyos V, Del Bufalo F, Yagyu S, Ando M, Dotti G, Suzuki M, Bouchier-Hayes L, Alemany R, Brenner MK.. " Mesenchymal Stromal Cells for Linked Delivery of Oncolytic and Apoptotic Adenoviruses to Non-small-cell Lung Cancers. " Mol Ther.. 2015 Sep ; 23 (9) : 1497-506.
Pubmed PMID: 26084970.
Nishio N, Diaconu I, Liu H, Cerullo V, Caruana I, Hoyos V, Bouchier-Hayes L, Savoldo B, Dotti G. " Armed oncolytic virus enhances immune functions of chimeric antigen receptor-modified T cells in solid tumors. " Cancer Res. 2014 Sep 15; 74 (18) : 5195-205.
Pubmed PMID: 25060519.
Collingridge DR, Glaser M, Osman S, Barthel H, Hutchinson OC, Luthra SK, Brady F, Bouchier-Hayes L, Martin SJ, Workman P, Price P, Aboagye EO. " In vitro selectivity, in vivo biodistribution and tumour uptake of annexin V radiolabelled with a positron emitting radioisotope. " Br. J. Cancer. 2003 Oct 6; 89 (7) : 1327-33.
Pubmed PMID: 14520468.
Glaser M, Collingridge DR, Aboagye EO, Bouchier-Hayes L, Hutchinson OC, Martin SJ, Price P, Brady F, Luthra SK. " Iodine-124 labelled annexin-V as a potential radiotracer to study apoptosis using positron emission tomography. " Appl Radiat Isot. 2003 Jan ; 58 (1) : 55-62.
Pubmed PMID: 12485664.
Keogh SA, Walczak H, Bouchier-Hayes L, Martin SJ. " Failure of Bcl-2 to block cytochrome c redistribution during TRAIL-induced apoptosis. " FEBS Lett.. 2000 Apr 7; 471 (1) : 93-8.
Pubmed PMID: 10760520.
Mynett-Johnson L, Kealey C, Claffey E, Curtis D, Bouchier-Hayes L, Powell C, McKeon P. " Multimarkerhaplotypes within the serotonin transporter gene suggest evidence of an association with bipolar disorder. " Am. J. Med. Genet.. 2000 Dec 4; 96 (6) : 845-9.
Pubmed PMID: 11121194.
Bouchier-Hayes L, Green DR. " Caspase-2: the orphan caspase. " Cell Death Differ.. 2012 Jan ; 19 (1) : 51-7.
Pubmed PMID: 22075987.
Bouchier-Hayes L, Sidi S.. " The nucleolus: A new home for the PIDDosome. " Cell Cycle. 2017 ; 16 : 1562-3.
Pubmed PMID: 28749201.
Bouchier-Hayes L. " The role of caspase-2 in stress-induced apoptosis. " J. Cell. Mol. Med.. 2010 Jun ; 14 (6) : 1212-24.
Pubmed PMID: 20158568.
Bouchier-Hayes L, Green DR. " Real time with caspase-2. " Cell Cycle. 2010 Jan 1; 9 (1) : 41611.
Pubmed PMID: 20016258.
Chipuk JE, Bouchier-Hayes L, Green DR. " Mitochondrial outer membrane permeabilization during apoptosis: the innocent bystander scenario. " Cell Death Differ.. 2006 Aug ; 13 (8) : 1396-402.
Pubmed PMID: 16710362.
Bouchier-Hayes L, Lartigue L, Newmeyer DD. " Mitochondria: pharmacological manipulation of cell death. " J. Clin. Invest.. 2005 Oct ; 115 (10) : 2640-7.
Pubmed PMID: 16200197.
Bouchier-Hayes L, Martin SJ. " CARDINAL roles in apoptosis and NFkappaB activation. " Vitam. Horm.. 2004 ; 67 : 133-47.
Pubmed PMID: 15110175.
Bouchier-Hayes L, Martin SJ. " CARD games in apoptosis and immunity. " EMBO Rep.. 2002 Jul ; 3 (7) : 616-21.
Pubmed PMID: 12101092.
Parsons MJ, Fassio SR, Bouchier-Hayes L.. " Detection of Initiator Caspase Induced Proximity in Single Cells by Caspase Bimolecular Fluorescence Complementation. " Methods Mol Biol.. 2016 ; 1419 : 41-56.
Pubmed PMID: 27108430.
Parsons MJ, Rehm M, Bouchier-Hayes L.. " Imaging-based methods for assessing caspase activity in single cells. " Cold Spring Harb Protoc.. 2015 Jan ; 5
Pubmed PMID: 25561626.
Rehm M, Parsons MJ, Bouchier-Hayes L.. " Measuring caspase activity by Förster resonance energy transfer. " Cold Spring Harb Protoc.. 2015 Jan ; 5
Pubmed PMID: 25561624.
Parsons MJ, Bouchier-Hayes L.. " Measuring initiator caspase activation by bimolecular fluorescence complementation. " Cold Spring Harb Protoc.. 2015 Jan ; 5
Pubmed PMID: 25561623.
Tait SW, Bouchier-Hayes L, Oberst A, Connell S, Green DR. " Live to dead cell imaging. " Methods Mol. Biol.. 2009 ; 559 : 33-48.
Pubmed PMID: 19609747.
Bouchier-Hayes L, Muñoz-Pinedo C, Connell S, Green DR. " Measuring apoptosis at the single cell level. " Methods. 2008 Mar ; 44 (3) : 222-8.
Pubmed PMID: 18314052.