Harold David Shine, Ph.D.

Professor Emeritus

(713) 898-6560

Email

hshine@bcm.edu

Positions

Professor Emeritus: Department of Neuroscience
Baylor College of Medicine
Houston, TX, US

Education

Post-Doctoral Fellowship at Harvard Medical School: 01/1983 - Boston, Massachusetts, United States

PhD from University Of Texas Medical School At Galveston: 01/1980 - Galveston, Texas, United States

MA from University Of Texas At Austin: 01/1975 - Austin, Texas, United States

BA from University Of Texas At Austin: 01/1973 - Austin, Texas, United States

BA from Texas Tech University: 09/1972 - Lubbock, Texas, United States

Professional Interests

Gene therapy of the nervous system
Spinal Cord Injury
Neuroimmunology of neural trauma

Professional Statement

The research interests of our laboratory focus on the molecular and cellular bases of disease and trauma of the nervous system with a translational emphasis. Our primary research emphasis is the study of the role of neurotrophic factors in CNS degeneration and regeneration as a consequence of trauma or neurodegenerative disease and their possible therapeutic use. Using viral vectors carrying genes for neurotrophic factors we have shown in animal models of CNS injury we can spare neurons from axotomy-induced neuronal death and support axonal sprouting after neural injury. Recently, we learned that neuroinflammation is required for regenerative effects of the neurotrophic factors and are now studying this phenomenon to determine its mechanism.

Techniques used in the laboratory span from gene cloning, through small animal neurosurgery, light and confocal microscopy, neuroimmunology, to animal behavior.

Websites

VIICTR Publications List

Selected Publications

Chen, Q and Shine, HD. " Neuroimmune processes associated with Wallerian degeneration support neurotrophin-3-induced axonal sprouting in the injured spinal cord.. " J Neurosci Res. 2013 ; 91 : 1280-1291.
Chen Q, Smith GM, Shine HD. " Immune activation is required for NT-3-induced axonal plasticity in chronic spinal cord injury. " Exp. Neurol.. 2008 Feb ; 209 (2) : 497-509.
Pubmed PMID: 18191837.
Chen Q, Zhou L, Shine HD. " Expression of neurotrophin-3 promotes axonal plasticity in the acute but not chronic injured spinal cord. " J. Neurotrauma. 2006 Aug ; 23 (8) : 1254-60.
Pubmed PMID: 16928183.
Zhou L, Shine HD. " Neurotrophic factors expressed in both cortex and spinal cord induce axonal plasticity after spinal cord injury. " J. Neurosci. Res.. 2003 Oct 15; 74 (2) : 221-6.
Pubmed PMID: 14515351.
Castro RF, Jackson KA, Goodell MA, Robertson CS, Liu H, Shine HD. " Failure of bone marrow cells to transdifferentiate into neural cells in vivo. " Science. 2002 Aug 23; 297 (5585) : 1299.
Pubmed PMID: 12193778.
Popko B, Puckett C, Lai E, Shine HD, Readhead C, Takahashi N, Hunt SW, Sidman RL, Hood L. " Myelin deficient mice: expression of myelin basic protein and generation of mice with varying levels of myelin. " Cell. 1987 Feb 27; 48 (4) : 713-21.
Pubmed PMID: 2434243.
Readhead C, Popko B, Takahashi N, Shine HD, Saavedra RA, Sidman RL, Hood L. " Expression of a myelin basic protein gene in transgenic shiverer mice: correction of the dysmyelinating phenotype. " Cell. 1987 Feb 27; 48 (4) : 703-12.
Pubmed PMID: 2434242.
Shine HD, Hertz L, De Vellis J, Haber B. " A fluorometric assay for gamma-glutamyl transpeptidase: demonstration enzymatic activity in cultured cells of neural origin. " Neurochem. Res.. 1981 Apr ; 6 (4) : 453-63.
Pubmed PMID: 6115328.
Shine HD, Perez-Polo JR. " A recombination assay of high molecular weight nerve growth factor protein complexes. " J. Neurochem.. 1976 Mar ; 26 (3) : 513-9.
Pubmed PMID: 1262875.
Goodman JC, Trask TW, Chen SH, Woo SL, Grossman RG, Carey KD, Hubbard GB, Carrier DA, Rajagopalan S, Aguilar-Cordova E, Shine HD. " Adenoviral-mediated thymidine kinase gene transfer into the primate brain followed by systemic ganciclovir: pathologic, radiologic, and molecular studies. " Hum. Gene Ther.. 1996 Jun 20; 7 (10) : 1241-50.
Pubmed PMID: 8793548.
Colak A, Goodman JC, Chen SH, Woo SL, Grossman RG, Shine HD. " Adenovirus-mediated gene therapy for experimental spinal cord tumors: tumoricidal efficacy and functional outcome. " Brain Res.. 1995 Sep 11; 691 (41276) : 76-82.
Pubmed PMID: 8590067.
Colak A, Goodman JC, Chen SH, Woo SL, Grossman RG, Shine HD. " Adenovirus-mediated gene therapy in an experimental model of breast cancer metastatic to the brain. " Hum. Gene Ther.. 1995 Oct ; 6 (10) : 1317-22.
Pubmed PMID: 8590736.
Perez-Cruet MJ, Trask TW, Chen SH, Goodman JC, Woo SL, Grossman RG, Shine HD. " Adenovirus-mediated gene therapy of experimental gliomas. " J. Neurosci. Res.. 1994 Nov 1; 39 (4) : 506-11.
Pubmed PMID: 7884826.
Kirkpatrick LL, Witt AS, Payne HR, Shine HD, Brady ST. " Changes in microtubule stability and density in myelin-deficient shiverer mouse CNS axons. " J. Neurosci.. 2001 Apr 1; 21 (7) : 2288-97.
Pubmed PMID: 11264304.
Macklis JD, Sidman RL, Shine HD. " Cross-linked collagen surface for cell culture that is stable, uniform, and optically superior to conventional surfaces. " In Vitro Cell. Dev. Biol.. 1985 Mar ; 21 (3) : 189-94.
Pubmed PMID: 3859483.
Shine HD, Harcourt PG, Sidman RL. " Cultured peripheral nervous system cells support peripheral nerve regeneration through tubes in the absence of distal nerve stump. " J. Neurosci. Res.. 1985 ; 14 (4) : 393-401.
Pubmed PMID: 4078938.
Jensen NA, Smith GM, Garvey JS, Shine HD, Hood L. " Cyclic AMP has a differentiative effect on an immortalized oligodendrocyte cell line. " J. Neurosci. Res.. 1993 Jun 15; 35 (3) : 288-96.
Pubmed PMID: 7688817.
Baumgartner BJ, Shine HD. " Permanent rescue of lesioned neonatal motoneurons and enhanced axonal regeneration by adenovirus-mediated expression of glial cell-line-derived neurotrophic factor. " J. Neurosci. Res.. 1998 Dec 15; 54 (6) : 766-77.
Pubmed PMID: 9856860.
Jensen NA, Smith GM, Shine HD, Garvey JS, Hood L. " Distinct hypomyelinated phenotypes in MBP-SV40 large T transgenic mice. " J. Neurosci. Res.. 1993 Feb 15; 34 (3) : 257-64.
Pubmed PMID: 7681114.
Sivaraman L, Gay J, Hilsenbeck SG, Shine HD, Conneely OM, Medina D, O'Malley BW. " Effect of selective ablation of proliferating mammary epithelial cells on MNU induced rat mammary tumorigenesis. " Breast Cancer Res. Treat.. 2002 May ; 73 (1) : 75-83.
Pubmed PMID: 12083633.
Chen SH, Shine HD, Goodman JC, Grossman RG, Woo SL. " Gene therapy for brain tumors: regression of experimental gliomas by adenovirus-mediated gene transfer in vivo. " Proc. Natl. Acad. Sci. U.S.A.. 1994 Apr 12; 91 (8) : 3054-7.
Pubmed PMID: 8159705.
Qi H, Shine HD, Li DQ, de Paiva CS, Farley WJ, Jones DB, Pflugfelder SC. " Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal epithelium in culture and the overexpression of GDNF in bioengineered constructs. " Exp. Eye Res.. 2008 Dec ; 87 (6) : 580-6.
Pubmed PMID: 18938159.
Shine HD, Haber B. " Immunocytochemical localization of gamma-glutamyl transpeptidase in the rat CNS. " Brain Res.. 1981 Aug 3; 217 (2) : 339-49.
Pubmed PMID: 6113879.
Smith GM, Shine HD. " Immunofluorescent labeling of tight junctions in the rat brain and spinal cord. " Int. J. Dev. Neurosci.. 1992 Oct ; 10 (5) : 387-92.
Pubmed PMID: 1492590.
Shine HD, Sidman RL. " Immunoreactive myelin basic proteins are not detected when shiverer mutant Schwann cells and fibroblasts are co-cultured with normal neurons. " J. Cell Biol.. 1984 Apr ; 98 (4) : 1291-5.
Pubmed PMID: 6201490.
Zou L, Yuan X, Long Y, Shine HD, Yang K. " Improvement of spatial learning and memory after adenovirus-mediated transfer of the nerve growth factor gene to aged rat brain. " Hum. Gene Ther.. 2002 Dec 10; 13 (18) : 2173-84.
Pubmed PMID: 12542848.
Grider MH, Mamounas LA, Le W, Shine HD. " In situ expression of brain-derived neurotrophic factor or neurotrophin-3 promotes sprouting of cortical serotonergic axons following a neurotoxic lesion. " J. Neurosci. Res.. 2005 Nov 1; 82 (3) : 404-12.
Pubmed PMID: 16206279.
Ayala GE, Wheeler TM, Shine HD, Schmelz M, Frolov A, Chakraborty S, Rowley D. " In vitro dorsal root ganglia and human prostate cell line interaction: redefining perineural invasion in prostate cancer. " Prostate. 2001 Nov 1; 49 (3) : 213-23.
Pubmed PMID: 11746267.
Grider MH, Park D, Spencer DM, Shine HD. " Lipid raft-targeted Akt promotes axonal branching and growth cone expansion via mTOR and Rac1, respectively. " J. Neurosci. Res.. 2009 Nov 1; 87 (14) : 3033-42.
Pubmed PMID: 19530170.
Shine HD, Readhead C, Popko B, Hood L, Sidman RL. " Morphometric analysis of normal, mutant, and transgenic CNS: correlation of myelin basic protein expression to myelinogenesis. " J. Neurochem.. 1992 Jan ; 58 (1) : 342-9.
Pubmed PMID: 1370079.
Shine HD, Readhead C, Popko B, Hood L, Sidman RL. " Myelin basic protein and myelinogenesis: morphometric analysis of normal, mutant and transgenic central nervous system. " Prog. Clin. Biol. Res.. 1990 ; 336 : 81-92.
Pubmed PMID: 1691511.
Qi H, Li DQ, Shine HD, Chen Z, Yoon KC, Jones DB, Pflugfelder SC. " Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells. " Exp. Eye Res.. 2008 Jan ; 86 (1) : 34-40.
Pubmed PMID: 17980361.
Baumgartner BJ, Shine HD. " Neuroprotection of spinal motoneurons following targeted transduction with an adenoviral vector carrying the gene for glial cell line-derived neurotrophic factor. " Exp. Neurol.. 1998 Sep ; 153 (1) : 102-12.
Pubmed PMID: 9743571.
Shine HD, Wyde PR, Aguilar-Cordova E, Chen SH, Woo SL, Grossman RG, Goodman JC. " Neurotoxicity of intracerebral injection of a replication-defective adenoviral vector in a semipermissive species (cotton rat). " Gene Ther.. 1997 Apr ; 4 (4) : 275-9.
Pubmed PMID: 9176511.
Zhou L, Baumgartner BJ, Hill-Felberg SJ, McGowen LR, Shine HD. " Neurotrophin-3 expressed in situ induces axonal plasticity in the adult injured spinal cord. " J. Neurosci.. 2003 Feb 15; 23 (4) : 1424-31.
Pubmed PMID: 12598631.
Sánchez I, Hassinger L, Paskevich PA, Shine HD, Nixon RA. " Oligodendroglia regulate the regional expansion of axon caliber and local accumulation of neurofilaments during development independently of myelin formation. " J. Neurosci.. 1996 Aug 15; 16 (16) : 5095-105.
Pubmed PMID: 8756439.
Qi H, Chuang EY, Yoon KC, de Paiva CS, Shine HD, Jones DB, Pflugfelder SC, Li DQ. " Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions. " Mol. Vis.. 2007 ; 13 : 1934-41.
Pubmed PMID: 17982417.
Rabinovsky ED, Smith GM, Browder DP, Shine HD, McManaman JL. " Peripheral nerve injury down-regulates CNTF expression in adult rat sciatic nerves. " J. Neurosci. Res.. 1992 Jan ; 31 (1) : 188-92.
Pubmed PMID: 1319503.
Trask TW, Trask RP, Aguilar-Cordova E, Shine HD, Wyde PR, Goodman JC, Hamilton WJ, Rojas-Martinez A, Chen SH, Woo SL, Grossman RG. " Phase I study of adenoviral delivery of the HSV-tk gene and ganciclovir administration in patients with current malignant brain tumors. " Mol. Ther.. 2000 Feb ; 1 (2) : 195-203.
Pubmed PMID: 10933931.
Olmsted-Davis EA, Gugala Z, Camargo F, Gannon FH, Jackson K, Kienstra KA, Shine HD, Lindsey RW, Hirschi KK, Goodell MA, Brenner MK, Davis AR. " Primitive adult hematopoietic stem cells can function as osteoblast precursors. " Proc. Natl. Acad. Sci. U.S.A.. 2003 Dec 23; 100 (26) : 15877-82.
Pubmed PMID: 14673088.
Ahmed N, Ratnayake M, Savoldo B, Perlaky L, Dotti G, Wels WS, Bhattacharjee MB, Gilbertson RJ, Shine HD, Weiss HL, Rooney CM, Heslop HE, Gottschalk S. " Regression of experimental medulloblastoma following transfer of HER2-specific T cells. " Cancer Res.. 2007 Jun 15; 67 (12) : 5957-64.
Pubmed PMID: 17575166.
Readhead C, Takasashi N, Shine HD, Saavedra R, Sidman R, Hood L. " Role of myelin basic protein in the formation of central nervous system myelin. " Ann. N. Y. Acad. Sci.. 1990 ; 605 : 280-5.
Pubmed PMID: 1702601.
Grider MH, Chen Q, Shine HD. " Semi-automated quantification of axonal densities in labeled CNS tissue. " J. Neurosci. Methods. 2006 Sep 15; 155 (2) : 172-9.
Pubmed PMID: 16469388.
Baumgartner BJ, Shine HD. " Targeted transduction of CNS neurons with adenoviral vectors carrying neurotrophic factor genes confers neuroprotection that exceeds the transduced population. " J. Neurosci.. 1997 Sep 1; 17 (17) : 6504-11.
Pubmed PMID: 9254662.
Smith GM, Rabinovsky ED, McManaman JL, Shine HD. " Temporal and spatial expression of ciliary neurotrophic factor after peripheral nerve injury. " Exp. Neurol.. 1993 Jun ; 121 (2) : 239-47.
Pubmed PMID: 8339774.
Shine HD, Perez-Polo JR. " 7S nerve growth factor protein in the golden hamster. " J. Neurochem.. 1976 Dec ; 27 (6) : 1315-8.
Pubmed PMID: 1003205.

Funding

Identification of T cell subtypes necessary for NT-3 induced neuroplasticity using a novel human-to-rat graft strategy: Mission Connect

Inflammation-associated factors that support neuroplasticity in chronic spinal cord injury: Christopher and Dana Reeve Foundation