Christopher Walkey, Ph.D.

Email

christopher.walkey@bcm.edu

Education

BSc from Simon Fraser University: 12/1992 - Burnaby, BC, Canada; Biochemistry

PhD from University of Alberta: 06/1998 - Edmonton, AB, Canada

Postdoctoral Fellowship at Dana-Farber Cancer Insitute and Harvard Medical School: Boston, Massachusetts, United States

Professional Interests

Somatic genome editing
Gene therapy and vector development
Lipid and lipoprotein metabolism

Selected Publications

Walkey CJ, Lagor WR. " CaMKIIδ gene editing- A base hit for the heart " J Cardiovasc Aging. 2023 Mar ; 3 (2) : 19.
Pubmed PMID: 37065676.
Huang Q, Chen AT, Chan KY, Sorensen H, Barry AJ, Azari B, Zheng Q, Beddow T, Zhao B, Tobey IG, Moncada-Reid C, Eid F, Walkey CJ, Ljungberg MC, Lagor WR, Heaney JD, Chan YA, Deverman BE. " Targeting AAV vectors to the central nervous system by engineering capsid-receptor interactions that enable crossing of the blood-brain barrier. " PLOS Biology. 2023 May ; in press
Lorincz R, Alvarez AB, Walkey CJ, Mendonça SA, Lu ZH, Martinez AE, Ljungberg C, Heaney JD, Lagor WR, Curiel DT. " In vivo editing of the pan-endothelium by immunity evading simian adenoviral vector " Biomed Pharmacother. 2023 Feb ; 158 : 114189.
Pubmed PMID: 36587560.
Phillips E, Ahmad N, Sun L, Iben J, Walkey CJ, Rusin A, Yuen T, Rosen CJ, Willis IM, Zaidi M, Johnson DL. " MAF1, a repressor of RNA polymerase III-dependent transcription, regulates bone mass " eLife. 2022 May ; 11 : e74740.
Pubmed PMID: 35611941.
Liang SQ, Walkey CJ, Martinez AE, Su Q, Dickinson ME, Wang D, Lagor WR, Heaney JD, Gao G, Xue W. " AAV5 delivery of CRISPR-Cas9 supports effective genome editing in mouse lung airway " Mol Ther. 2022 Jan ; 30 (1) : 238-243.
Pubmed PMID: 34695545.
Doerfler AM, Walkey CJ, Lagor WR. " Recent advances in genome editing for cardiovascular disease " Curr Opin Cardiol. 2020 May ; 35 (3) : 242-248.
Pubmed PMID: 32073405.
Wang X, Walkey CJ, Maretti-Mira AC, Wang L, Johnson DL, DeLeve LD. " Susceptibility of Rat Steatotic Liver to Ischemia-Reperfusion Is Treatable With Liver-Selective Matrix Metalloproteinase Inhibition " Hepatology. 2020 Oct ; 72 (5) : 1771-1785.
Pubmed PMID: 32060938.
Wang X, Rusin A, Walkey CJ, Lin JJ, Johnson DL. " The RNA polymerase III repressor MAF1 is regulated by ubiquitin-dependent proteasome degradation and modulates cancer drug resistance and apoptosis " J Biol Chem. 2019 Dec ; 294 (50) : 19255-19268.
Pubmed PMID: 31645432.
Chen CY, Lanz RB, Walkey CJ, Chang WH, Lu W, Johnson DL. " Maf1 and Repression of RNA Polymerase III-Mediated Transcription Drive Adipocyte Differentiation " Cell Rep. 2018 Aug ; 24 (7) : 1852-1864.
Pubmed PMID: 30110641.
Johnson SAS, Lin JJ, Walkey CJ, Leathers MP, Coarfa C, Johnson DL. " Elevated TATA-binding protein expression drives vascular endothelial growth factor expression in colon cancer " Oncotarget. 2017 Jul ; 8 (30) : 48832-48845.
Pubmed PMID: 28415573.
Palmer EE, Jarrett KE, Sachdev RK, Al Zahrani F, Hashem MO, Ibrahim N, Sampaio H, Kandula T, Macintosh R, Gupta R, Conlon DM, Billheimer JT, Rader DJ, Funato K, Walkey CJ, Lee CS, Loo C, Brammah S, Elakis G, Zhu Y, Buckley M, Kirk EP,..., Lagor WR. " Neuronal deficiency of ARV1 causes an autosomal recessive epileptic encephalopathy " Mol Genet. 2016 Jul ; 25 (14) : 3042-3054.
Pubmed PMID: 27270415.
Liu Y, Walkey CJ, Green TJ, van Vuuren HJ, Kitts DD. " Enhancing the natural folate level in wine using bioengineering and stabilization strategies " Food Chem. 2016 Mar ; 194 : 26-31.
Pubmed PMID: 26471523.
Bessonov K, Walkey CJ, Shelp BJ, van Vuuren HJ, Chiu D, van der Merwe G. " Functional analyses of NSF1 in wine yeast using interconnected correlation clustering and molecular analyses " PLoS One. 2013 Oct ; 8 (10) : e77192.
Pubmed PMID: 24130853.
Luo Z, Walkey CJ, Madilao LL, Measday V, Van Vuuren HJ. " Functional improvement of Saccharomyces cerevisiae to reduce volatile acidity in wine " FEMS Yeast Res. 2013 Aug ; 13 (5) : 485-94.
Pubmed PMID: 23692528.
Walkey CJ, Luo Z, Madilao LL, van Vuuren HJ. " The fermentation stress response protein Aaf1p/Yml081Wp regulates acetate production in Saccharomyces cerevisiae " PLoS One. 2012 Dec ; 7 (12) : e51551.
Pubmed PMID: 23240040.
Walkey CJ, Luo Z, Borchers CH, Measday V, van Vuuren HJ. " The Saccharomyces cerevisiae fermentation stress response protein Igd1p/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzyme " FEMS Yeast Res. 2011 Sep ; 11 (6) : 499-508.
Pubmed PMID: 21585652.
Walkey CJ, Spiegelman BM. " A functional peroxisome proliferator-activated receptor-gamma ligand-binding domain is not required for adipogenesis " J Biol Chem. 2008 Sep ; 283 (36) : 24290-4.
Pubmed PMID: 18622018.
Puigserver P, Rhee J, Donovan J, Walkey CJ, Yoon JC, Oriente F, Kitamura Y, Altomonte J, Dong H, Accili D, Spiegelman BM. " Insulin-regulated hepatic gluconeogenesis through FOXO1-PGC-1alpha interaction " Nature. 2003 May ; 423 (6939) : 550-5.
Pubmed PMID: 12754525.
Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. " Transcriptional regulation of adipogenesis " Genes Dev. 2000 Jun ; 14 (11) : 1293-307.
Pubmed PMID: 10837022.
Walkey CJ, Shields DJ, Vance DE. " Identification of three novel cDNAs for human phosphatidylethanolamine N-methyltransferase and localization of the human gene on chromosome 17p11.2 " Biochim Biophys Acta. 1999 Jan ; 1436 (3) : 405-12.
Pubmed PMID: 9989271.
Agellon LB, Walkey CJ, Vance DE, Kuipers F, Verkade HJ. " The unique acyl chain specificity of biliary phosphatidylcholines in mice is independent of their biosynthetic origin in the liver " Hepatology. 1999 Sep ; 30 (3) : 725-9.
Pubmed PMID: 10462379.
Vance DE, Walkey CJ, Agellon LB. " Why has phosphatidylethanolamine N-methyltransferase survived in evolution? " Biochem Soc Trans. 1998 Aug ; 26 (3) : 337-40.
Pubmed PMID: 9765874.
Walkey CJ, Yu L, Agellon LB, Vance DE. " Biochemical and evolutionary significance of phospholipid methylation " J Biol Chem. 1998 Oct ; 273 (42) : 27043-6.
Pubmed PMID: 9765216.
Vance DE, Walkey CJ. " Roles for the methylation of phosphatidylethanolamine " Curr Opin Lipidol. 1998 Apr ; 9 (2) : 125-30.
Pubmed PMID: 9559269.
Walkey CJ, Donohue LR, Bronson R, Agellon LB, Vance DE. " Disruption of the murine gene encoding phosphatidylethanolamine N-methyltransferase " Proc Natl Acad Sci U S A. 1997 Nov ; 94 (24) : 12880-5.
Pubmed PMID: 9371769.
Vance DE, Walkey CJ, Cui Z. " Phosphatidylethanolamine N-methyltransferase from liver " Biochim Biophys Acta. 1997 Sep ; 1348 (1-2) : 142-50.
Pubmed PMID: 9370326.
Walkey CJ, Cui Z, Agellon LB, Vance DE. " Characterization of the murine phosphatidylethanolamine N-methyltransferase-2 gene " J Lipid Res. 1996 Nov ; 37 (11) : 2341-50.
Pubmed PMID: 8978486.
Walkey CJ, Kalmar GB, Cornell RB. " Overexpression of rat liver CTP:phosphocholine cytidylyltransferase accelerates phosphatidylcholine synthesis and degradation " J Biol Chem. 1994 Feb ; 269 (8) : 5742-9.
Pubmed PMID: 8119913.
Johnson JE, Kalmar GB, Sohal PS, Walkey CJ, Yamashita S, Cornell RB. " Comparison of the lipid regulation of yeast and rat CTP: phosphocholine cytidylyltransferase expressed in COS cells " Biochem J. 1992 Aug ; 285 (3) : 815-20.
Pubmed PMID: 1323275.

Memberships

American Society for Gene and Cell Therapy