Anil Kumar Panigrahi, Ph.D.

Assistant Professor

Email

panigrah@bcm.edu

Positions

Assistant Professor: Molecular & Cellular Biology
Baylor College of Medicine
Houston, TX, US

Professional Interests

Mechanism of enhancer action; Cell-free assays for transcription; Proteomics of transcriptional dynamics

Professional Statement

I recognize the fact that many questions in biology cannot be answered in their strictly cellular or organismal contexts, necessitating development of cell-free (in vitro) approaches. I have been always interested in understanding the intricacies of eukaryotic transcription regulation. I began my doctoral training in India by developing assays for ATP-dependent chromatin remodeling. Here at BCM, I developed cell-free assays to quantify enhancer-promoter communications and consequent transcription activation. I aim to employ these assays to further understand the proteomic dynamics at the enhancer-promoter contacts that result in actual transcriptional potentiation. Additionally, my work has provided evidence that different regions within a gene's coding and regulatory domains physically and dynamically interact during the gene's transcription. I aim to further understand how and why such interactions occur.

Websites

VIICTR Publications List

Selected Publications

Panigrahi A.K., Foulds C.E., Lanz R.B., Hamilton R.A., Yi P., Lonard D.M., Tsai M-J., Tsai S.Y., O’Malley B.W.. " SRC-3 governs dynamic estrogen-induced chromatin looping interactions during transcription " Molec Cell. 2018 ; 70 : 679-694.e7.
Pubmed PMID: 29775582.
Bader D., Hartig S., Putluri V., Foley S., Hamilton M., Smith E., Saha P., Panigrahi A., Walker C., Zong L., Martini-Stoica H., Chen R., Rajapakshe K., Coarfa C., Sreekumar A., Mitsiades N., Bankson J., Ittmann M., O'Malley B.W., Putluri N. & McGuire S.. " Mitochondrial Pyruvate Import is a Metabolic Vulnerability in AR-Driven Prostate Cancer. " Nature Metabolism. 2019 ; 1 : 70-85.
Pubmed PMID: 31198906.
Panigrahi AK, Zhang N, Mao Q, Pati D. " Calpain-1 cleaves rad21 to promote sister chromatid separation. " Mol. Cell. Biol.. 2011 Nov ; 31 (21) : 4335-47.
Pubmed PMID: 21876002.
Panigrahi AK, Zhang N, Otta SK, Pati D. " A cohesin-RAD21 interactome. " Biochem. J.. 2012 Mar 15; 442 (3) : 661-70.
Pubmed PMID: 22145905.
Basu D, Zhang N, Panigrahi AK, Horton TM, Pati D. " Development and validation of a fluorogenic assay to measure separase enzyme activity. " Anal. Biochem.. 2009 Sep 15; 392 (2) : 133-8.
Pubmed PMID: 19497291.
Panigrahi AK, Pati D. " Higher-order orchestration of hematopoiesis: Is cohesin a new player? " Exp. Hematol.. 2012 Dec ; 40 (12) : 967-73.
Pubmed PMID: 23022223.
Meyer R, Fofanov V, Panigrahi A, Merchant F, Zhang N, Pati D. " Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers. " Clin. Cancer Res.. 2009 Apr 15; 15 (8) : 2703-10.
Pubmed PMID: 19351757.
Panigrahi AK, Pati D. " Road to the crossroads of life and death: linking sister chromatid cohesion and separation to aneuploidy, apoptosis and cancer. " Crit. Rev. Oncol. Hematol.. 2009 Dec ; 72 (3) : 181-93.
Pubmed PMID: 19162508.
Zhang N, Panigrahi AK, Mao Q, Pati D. " Interaction of Sororin Protein with Polo-like Kinase 1 Mediates Resolution of Chromosomal Arm Cohesion. " J. Biol. Chem.. 2011 Dec 2; 286 (48) : 41826-37.
Pubmed PMID: 21987589.
Panigrahi AK, Tomar RS, Chaturvedi MM. " Mechanism of nucleosome disruption and octamer transfer by the chicken SWI/SNF-like complex. " Biochem. Biophys. Res. Commun.. 2003 Jun 20; 306 (1) : 72-8.
Pubmed PMID: 12788068.
Panigrahi AK, Tomar RS, Chaturvedi MM. " A SWI/SNF-like factor from chicken liver that disrupts nucleosomes and transfers histone octamers in cis and trans. " Arch. Biochem. Biophys.. 2003 Jun 1; 414 (1) : 24-33.
Pubmed PMID: 12745251.
Gordon M., Holt D., Panigrahi A., Wilhelm B., Erdjument-Bromage H., Tempst P., Bahler J. & Cairns B.. " Genome-wide dynamics of SAPHIRE, an essential complex for gene activation and chromatin boundaries " Mol Cell Biol.. 2007 ; 27 : 4058-4069.
Pubmed PMID: 17371846.
Anil Panigrahi; Bert W. O'Malley. " Mechanisms of enhancer action: the known and the unknown " Genome Biol.. 2021 ; 22 (1)
Pubmed PMID: 33858480.