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Center for Cell and Gene Therapy

Flow Cytometry Facility

Master
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The Flow Cytometry Facility provides multi-parameter cellular analysis and viability assessment using state-of-the-art equipment:

  • BDIS Canto-I, 2 laser-6 color cytometer
  • BDIS Canto-II, 3 laser-8 color cytometer
  • BDIS FACScan, 1 laser-3 color cytometer
  • BDIS Lyse-Wash Assistent (X3
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Qualifications

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  • Accredited by the College of American Pathologists
  • CLIA certified as a high-complexity laboratory
  • Served as reference laboratory for National Marrow Donor Program
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Staff Experience

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The staff offers experience in:

  • Evaluation of CD34+ stem cells in peripheral blood, bone marrow, and umbilical cord blood
  • Qualitative and quantitative assessment of specific cellular doses for transplant
  • Analysis of the efficacy of cell specific purging and selection procedures
  • Immunophenotyping of therapeutic cell populations used in clinical trials
  • Evaluation of immune reconstitution of patients following infusion of grafts and therapeutic cell populations
  • Assistance in the development and implementation of new cell processing and culture procedures
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Additional Services

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Additional services include:

  • Analysis and interpretation of data
  • Provide final reports including statistical analysis, graphic and visual interpretation
  • Establishment and maintenance of protocol specific databases
  • Development of prediction algorithms for cell specific collection, purging and selection procedures
  • Provision of training for visiting investigators and technical staff who are establishing stem cell laboratories
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For additional information contact April G. Durett.