About the Core
The Tissue Culture Core Laboratory maintains active cell cultures of many types of mammalian cell lines as well as frozen stocks of a larger cell library deposited by BCM and Molecular and Cellular Biology Department investigators. We also carry stocks of media, reagents, and disposable tissue culture supplies for purchase by core users.
Services Offered
- Maintaining Cultured Cell Lines
- Plating of Requested Cells (96 wells up to 15 cm plates)
- Mycoplasma Testing
- STR Analysis Profile (Cell Line DNA Fingerprinting) samples are sent to a collaborating lab
- Training on Culturing & Maintenance of Growing Cell Lines
- Cell Culture Medium (GIBCO) & Custom Specialty Medium
- FBS & Charcoal Stripped FBS
- Reagents & Specialty supplements for media preparation
- Tissue Culture Disposable Plasticware
- Single Cell Cloning
- Primary Cell Culture
- Frozen Cell Vial from our Library
- PBMC and magnetically-isolated cells sourced from blood donors through Gulf Coast Regional Blood Center
- Nucleofection of plasmids or CRISPR/Cas ribonucleoprotein (RNP) complexes for gene editing
Core Equipment
- Nikon Bright Field and Florescent Microscope with Camera
- Cell Countess (imaging and cell counter)
- Osmometer
- 100 cubic feet of incubator space
- Four sterile-hood workspaces (BSL2 biosafety cabinets)
- EVOS inverted microscope with fluorescence and digital camera
- Lonza 4D-Nucleofector Core, X, and LV Units
- MultiMACS Cell24 Separator
Location and Contact
Baylor College of Medicine, Main Campus
Building: BCM Michael DeBakey Center
Tissue Culture Room: M830
Lab/Office Room: M825
Phone: 713-798-6236
Email: tccmail@bcm.edu