Patient-Derived Xenograft and Advanced In Vivo Models Core Services

PDXO 3D Culture in Matrigel. Maintenance and/or expansion of active PDX-derived organoids growing in Matrigel.

PDO and PDXO-PDX derived tumor organoid culture. Generation of tumor organoid models from human patient or PDX tissue sources.

Cryopreserved PDXO distribution. Viably frozen tumor organoid model distribution to investigators.

PDO and PDXO Characterization – Tier 1. Assisted tumor organoid model characterization including STR fingerprinting, biomarker expression, and tumor gene expression.

PDO and PDXO Characterization – Tier 2. Assisted tumor organoid model characterization including cytometer-sorted tumor cell enrichment and stromal cell elimination.

GentleMACS automated tissue dissociation. Tumor dissociation/homogenization instrument operation available to Core users (or assisted use from Core staff).

IVIS bioluminescence imaging instrument. Instrument operation available to Core users independently (with appropriate training) or with assisted use. Instrument can only be used if a reservation has been made with Core staff.

Ovivo. Conversion of 2D cells to 3D structures on CAM. Includes egg incubation, egg preparation, and tumor harvest. Does not include imaging or report.

Ovotar. Patient Derived Xenograft establishment on CAM. Includes egg incubation, egg preparation, and tumor harvest. Does not include imaging or report.

OvoQuest. Custom bioassay kit that includes 9 eggs and 2h of tech time. This type of request includes egg incubation, egg preparation, tumor harvest, imaging and report building for 9 eggs. 

Technician Time. Technical assistance. Applies to report building, tissue harvest, brightfield imaging, invasion analysis, angiogenesis analysis, etc.

Fees:

Breeder Mice: Excess male mice from the Core’s SCID/Bg breeding colony are available for purchase.

PDX Generation: Transplantation of human tissue to generate a PDX model.

Viably Frozen Tissue: Viably Frozen tissue for Tissue for PDX Propagation: viably frozen or fresh tissue for re-generation of a PDX model. 

Fresh Tissue for Assays: Fresh tissue for end-point in vitro assays.

Snap Frozen Tissue: Snap frozen PDX tissue for molecular assays.

FFPE Unstained Slides: Formalin-fixed paraffin embedded tissue of a single PDX model for IHC or IF staining.

TMA Unstained Slides: Tissue microarray of the BCM breast cancer model collection for IHC or IF staining.

Technician Time: Technical assistance which applies to training/guidance on various animal techniques, study design assistance, etc.

Fees: