Positions
- Professor
-
Biochemistry and Molecular Pharmacology
Baylor College of Medicine
Houston, TX US
Primary Appointment
- Charles C. Bell Jr. Professor of Structural Biology
-
Biochemistry and Molecular Pharmacology
Baylor College of Medicine
- Director
-
CryoEM/ET Core
Baylor College of Medicine
- Co-Director
-
Center for Computational and Integrative Biomedical Research (CIBR)
Baylor College of Medicine
Houston, Texas United States
- Member
-
Dan L Duncan Comprehensive Cancer Center
Baylor College of Medicine
- Deputy Director
-
Advanced Technical Cores
Baylor College of Medicine
Addresses
- Office (Office)
-
Baylor College of Medicine
BCMN-N420
Houston, TX 77030
United States
Phone: (713) 798-9020
sludtke@bcm.edu
Education
- PhD from Rice University
- 06/1996 - Houston, Texas United States
- Physics
- MA from Rice University
- 06/1993 - Houston, Texas United States
- Physics
- BS from Caltech
- 06/1990 - Pasadena, California United States
- Physics
Honors & Awards
- Burton Medal
- Microscopy Society of America (01/2008)
- HAMBP (Houston Area Molecular Biophysics Program) Fellow
- Houston Area Molecular Biophysics Program (01/1992 - 01/1996)
- NRSA (Institutional) Fellow
- NRSA (01/1996 - 01/1997)
- NRSA (Individual) Fellow
- NRSA (01/1998 - 01/1999)
- Outstanding Lecturer in Quantitative and Computational Biosciences
- Outstanding graduate program lecturer in 2020 and 2023
- BCM, GSBS
Professional Interests
- Cryo-electron Microscopy and Tomography
- Quantitative Image Processing Algorithm Development
- Structural Biology and Macromolecular Assemblies
- Membranes and Membrane Proteins
- Protein Structure-Function and Protein Folding
- Antibiotic Resistance
- Fatty Acids and Lipid Metabolism
Professional Statement
My group focuses on CryoEM/CryoET and quantitative image processing, applying these techniques to a wide range of biological systems ranging from individual macromolecules to whole cells. For the last 25 years my group has been developing the EMAN software suite for both high resolution single particle analysis as well as in-situ and in-vitro tomography. This software has been used on a large fraction of the structures in the EMDatabank, and with over 30,000 downloads is one of the most widely used in the field. In addition to developing new image processing methods such as deep learning applications and bispectral analysis, my group engages in a wide range of biological projects, from the high resolution structure and dynamics of individual molecules to the study of functioning macromolecular assemblies within the cell. Over the last decade, cryoEM has experienced a renaissance, largely due to the development of revolutionary direct detector technology, making this field directly competitive with X-ray crystallography for high resolution structural analysis, and providing many unique capabilities, such as in-situ structural biology. Now, we are seeing a similar growth pattern in CryoET which permits visualization of macromolecules within cells without chemical fixation, opening the door for a new era of in-situ structural biology.Websites
Videos
Selected Publications
- Chen M, Dai W, Sun SY, Jonasch D, He CY, Schmid MF, Chiu W, Ludtke SJ "Convolutional neural networks for automated annotation of cellular cryo-electron tomograms." Nature Meth.. 2017;14(10):983-985. Pubmed PMID: 28846087
- Yi P, Wang Z, Feng Q, Chou CK, Pintilie GD, Shen H, Foulds CE, Fan G, Serysheva I, Ludtke SJ, Schmid MF, Hung MC, Chiu W, O'Malley BW "Structural and Functional Impacts of ER Coactivator Sequential Recruitment." Mol. Cell. 2017;67(5):733-743. Pubmed PMID: 28844863
- J.M. Bell, M. Chen, P.R. Baldwin & S.J. Ludtke "High Resolution Single Particle Refinement in EMAN2.1." Methods. 2016;100:25-34. Pubmed PMID: 26931650
- R.N. Irobalieva, J.M. Fogg, D.J. Catanese, T. Sutthibutpong, M. Chen, A.K. Barker, S.J. Ludtke, S.A. Harris, M.F. Schmid, W. Chiu & L. Zechiedrich "Structural diversity of supercoiled DNA." Nat. Commun.. 2015;6:8440. Pubmed PMID: 26455586
- G. Fan, M.L. Baker, Z. Wang, M.R. Baker, P.A. Sinyagovskiy, W. Chiu, S.J. Ludtke & I.I. Serysheva "Gating machinery of InsP3R channels revealed by electron cryomicroscopy." Nature. 2015;527:336-341. Pubmed PMID: 26458101
- Fluty AC, Ludtke SJ "Precision requirements and data compression in CryoEM/CryoET." J Struct Biol. 2022 Sep;214:107875. Pubmed PMID: 35724904
- Fan G, Baker MR, Terry LE, Arige V, Chen M, Serysheva AB, Baker ML, Ludtke SJ, Yule DI, Serysheva II "Conformational motions and ligand-binding underlying gating and regulation in IP3R channel." Nat Commun. 2022 Nov 14;13:6942. Pubmed PMID: 36376291
- Chen M, Ludtke SJ "Deep learning-based mixed-dimensional Gaussian mixture model for characterizing variability in cryo-EM." Nat Methods. 2021 Aug;18:930-936. Pubmed PMID: 34326541
- Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, ..., Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP "Comprehensive structure and functional adaptations of the yeast nuclear pore complex." Cell. 2022 Jan 20;185:361-378. Pubmed PMID: 34982960
Memberships
- Biophysical Society
- Member (01/1992)
- Microscopy Society of America
- Member (01/1998)
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