Positions
- Professor
-
Pediatrics-Hem-Onc and Center for Cell & Gene Therapy
Baylor College of Medicine
Houston, TX US
- Member
-
Dan L Duncan Comprehensive Cancer Center
Baylor College of Medicine
Houston, Texas United States
Education
- PhD from University Of Birmingham
- 01/2002 - Birmingham, United Kingdom
- PhD from Baylor College Of Medicine
- 01/2005 - Houston, TX United States
Professional Interests
- T cell immunotherapy for viruses and cancer
Professional Statement
Dr. Leen's research interests involve using cytotoxic T cells (CTL) for viral and malignant diseases. Dr. Leen is a co-principal investigator on a clinical trial assessing the safety of adoptively transferred donor-derived virus-specific CTL for the prophylaxis and treatment of adenoviral infections post allogeneic stem cell transplant. She is also developing and optimizing methods for generating tumor-specific CTL directed against the cancer testis antigens SSX2 and SSX4, which are expressed in a variety of tumors including Hodgkin Reed Sternberg cells. Therefore, the development of effective methods to reactivate and expand tumor-specific T cells directed against these antigens will lead to the development of immunotherapy protocols for the treatment of patients with relapsed EBV negative Hodgkin's disease and non-Hodgkin Lymphoma.Websites
Selected Publications
- Ildefonso CJ, Kong L, Leen A, Chai SJ, Petrochelli V, Chintagumpala M, Hurwitz MY, Chévez-Barrios P, Hurwitz RL "Absence of systemic immune response to adenovectors after intraocular administration to children with retinoblastoma.." Mol. Ther.. 2010 Oct;18(10):1885-90. Pubmed PMID: 20606647
- Vera JF, Brenner LJ, Gerdemann U, Ngo MC, Sili U, Liu H, Wilson J, Dotti G, Heslop HE, Leen AM, Rooney CM "Accelerated production of antigen-specific T cells for preclinical and clinical applications using gas-permeable rapid expansion cultureware (G-Rex).." J. Immunother.. 2010 Apr;33(3):305-15. Pubmed PMID: 20445351
- Leen AM, Bollard CM, Myers GD, Rooney CM "Adenoviral infections in hematopoietic stem cell transplantation.." Biol. Blood Marrow Transplant.. 2006 Mar;12(3):243-51. Pubmed PMID: 16503493
- Leen AM, Rooney CM "Adenovirus as an emerging pathogen in immunocompromised patients.." Br. J. Haematol.. 2005 Jan;128(2):135-44. Pubmed PMID: 15638847
- Bollard CM, Kuehnle I, Leen A, Rooney CM, Heslop HE "Adoptive immunotherapy for posttransplantation viral infections.." Biol. Blood Marrow Transplant.. 2004 Mar;10(3):143-55. Pubmed PMID: 14993880
- Cruz CR, Hanley PJ, Liu H, Torrano V, Lin YF, Arce JA, Gottschalk S, Savoldo B, Dotti G, Louis CU, Leen AM, Gee AP, Rooney CM, Brenner MK, Bollard CM, Heslop HE "Adverse events following infusion of T cells for adoptive immunotherapy: a 10-year experience.." Cytotherapy. 2010 Oct;12(6):743-9. Pubmed PMID: 20429793
- Melenhorst JJ, Leen AM, Bollard CM, Quigley MF, Price DA, Rooney CM, Brenner MK, Barrett AJ, Heslop HE "Allogeneic virus-specific T cells with HLA alloreactivity do not produce GVHD in human subjects.." Blood. 2010 Nov 25;116(22):4700-2. Pubmed PMID: 20709906
- Shafer JA, Cruz CR, Leen AM, Ku S, Lu A, Rousseau A, Heslop HE, Rooney CM, Bollard CM, Foster AE "Antigen-specific cytotoxic T lymphocytes can target chemoresistant side-population tumor cells in Hodgkin lymphoma.." Leuk. Lymphoma. 2010 May;51(5):870-80. Pubmed PMID: 20367572
- Foster AE, Leen AM, Lee T, Okamura T, Lu A, Vera J, Atkinson R, Bollard CM, Dotti G, Rooney CM "Autologous designer antigen-presenting cells by gene modification of T lymphocyte blasts with IL-7 and IL-12.." J. Immunother..;30(5):506-16. Pubmed PMID: 17589291
- Yang H, Robinson SN, Lu J, Decker WK, Xing D, Steiner D, Parmar S, Shah N, Champlin RE, Munsell M, Leen A, Bollard C, Simmons PJ, Shpall EJ "CD3(+) and/or CD14(+) depletion from cord blood mononuclear cells before ex vivo expansion culture improves total nucleated cell and CD34(+) cell yields.." Bone Marrow Transplant.. 2010 Jun;45(6):1000-7. Pubmed PMID: 19838220
- Long HM, Haigh TA, Gudgeon NH, Leen AM, Tsang CW, Brooks J, Landais E, Houssaint E, Lee SP, Rickinson AB, Taylor GS "CD4+ T-cell responses to Epstein-Barr virus (EBV) latent-cycle antigens and the recognition of EBV-transformed lymphoblastoid cell lines.." J. Virol.. 2005 Apr;79(8):4896-907. Pubmed PMID: 15795275
- Leen AM, Tripic T, Rooney CM "Challenges of T cell therapies for virus-associated diseases after hematopoietic stem cell transplantation.." Expert Opin Biol Ther. 2010 Mar;10(3):337-51. Pubmed PMID: 20132056
- Straathof KC, Leen AM, Buza EL, Taylor G, Huls MH, Heslop HE, Rooney CM, Bollard CM "Characterization of latent membrane protein 2 specificity in CTL lines from patients with EBV-positive nasopharyngeal carcinoma and lymphoma.." J. Immunol.. 2005 Sep 15;175(6):4137-47. Pubmed PMID: 16148164
- Sanchez C, Chan R, Bajgain P, Rambally S, Palapattu G, Mims M, Rooney CM, Leen AM, Brenner MK, Vera JF "Combining T-cell immunotherapy and anti-androgen therapy for prostate cancer.." Prostate Cancer Prostatic Dis.. 2013 Jun;16(2):123-31. Pubmed PMID: 23295316
- Bollard CM, Gottschalk S, Leen AM, Weiss H, Straathof KC, Carrum G, Khalil M, Wu MF, Huls MH, Chang CC, Gresik MV, Gee AP, Brenner MK, Rooney CM, Heslop HE "Complete responses of relapsed lymphoma following genetic modification of tumor-antigen presenting cells and T-lymphocyte transfer.." Blood. 2007 Oct 15;110(8):2838-45. Pubmed PMID: 17609424
- Leen AM, Sili U, Vanin EF, Jewell AM, Xie W, Vignali D, Piedra PA, Brenner MK, Rooney CM "Conserved CTL epitopes on the adenovirus hexon protein expand subgroup cross-reactive and subgroup-specific CD8+ T cells.." Blood. 2004 Oct 15;104(8):2432-40. Pubmed PMID: 15265797
- Leen A, Ratnayake M, Foster A, Heym K, Ahmed N, Rooney CM, Gottschalk S "Contact-activated monocytes: efficient antigen presenting cells for the stimulation of antigen-specific T cells.." J. Immunother.. 2007 Jan;30(1):96-107. Pubmed PMID: 17198088
- Leen AM, Christin A, Myers GD, Liu H, Cruz CR, Hanley PJ, Kennedy-Nasser AA, Leung KS, Gee AP, Krance RA, Brenner MK, Heslop HE, Rooney CM, Bollard CM "Cytotoxic T lymphocyte therapy with donor T cells prevents and treats adenovirus and Epstein-Barr virus infections after haploidentical and matched unrelated stem cell transplantation.." Blood. 2009 Nov 5;114(19):4283-92. Pubmed PMID: 19700662
- Leen AM, Heslop HE "Cytotoxic T lymphocytes as immune-therapy in haematological practice.." Br. J. Haematol.. 2008 Oct;143(2):169-79. Pubmed PMID: 18691164
- Gerdemann U, Katari U, Christin AS, Cruz CR, Tripic T, Rousseau A, Gottschalk SM, Savoldo B, Vera JF, Heslop HE, Brenner MK, Bollard CM, Rooney CM, Leen AM "Cytotoxic T Lymphocytes Simultaneously Targeting Multiple Tumor-associated Antigens to Treat EBV Negative Lymphoma.." Mol. Ther.. 2011 Dec;19(12):2258-68. Pubmed PMID: 21915103
- Micklethwaite KP, Savoldo B, Hanley PJ, Leen AM, Demmler-Harrison GJ, Cooper LJ, Liu H, Gee AP, Shpall EJ, Rooney CM, Heslop HE, Brenner MK, Bollard CM, Dotti G "Derivation of human T lymphocytes from cord blood and peripheral blood with antiviral and antileukemic specificity from a single culture as protection against infection and relapse after stem cell transplantation.." Blood. 2010 Apr 1;115(13):2695-703. Pubmed PMID: 20110422
- Leen A, Meij P, Redchenko I, Middeldorp J, Bloemena E, Rickinson A, Blake N "Differential immunogenicity of Epstein-Barr virus latent-cycle proteins for human CD4(+) T-helper 1 responses.." J. Virol.. 2001 Sep;75(18):8649-59. Pubmed PMID: 11507210
- Katari UL, Keirnan JM, Worth AC, Hodges SE, Leen AM, Fisher WE, Vera JF "Engineered T cells for pancreatic cancer treatment.." HPB (Oxford). 2011 Sep;13(9):643-50. Pubmed PMID: 21843265
- van Den Brink M, Leen AM, Baird K, Merchant M, Mackall C, Bollard CM "Enhancing immune reconstitution: from bench to bedside.." Biol. Blood Marrow Transplant.. 2013 Jan;19(1):S79-83. Pubmed PMID: 23041603
- Raval M, Gulbis A, Bollard C, Leen A, Chemaly R, Shpall E, Lahoti A, Kebriaei P "Evaluation and management of BK virus-associated nephropathy following allogeneic hematopoietic cell transplantation.." Biol. Blood Marrow Transplant.. 2011 Nov;17(11):1589-93. Pubmed PMID: 21767514
- Fujita Y, Leen AM, Sun J, Nakazawa Y, Yvon E, Heslop HE, Brenner MK, Rooney CM "Exploiting cytokine secretion to rapidly produce multivirus-specific T cells for adoptive immunotherapy.." J. Immunother.. 2008 Sep;31(7):665-74. Pubmed PMID: 18600178
- Leen AM, Sili U, Savoldo B, Jewell AM, Piedra PA, Brenner MK, Rooney CM "Fiber-modified adenoviruses generate subgroup cross-reactive, adenovirus-specific cytotoxic T lymphocytes for therapeutic applications.." Blood. 2004 Feb 1;103(3):1011-9. Pubmed PMID: 14525768
- Hanley PJ, Cruz CR, Savoldo B, Leen AM, Stanojevic M, Khalil M, Decker W, Molldrem JJ, Liu H, Gee AP, Rooney CM, Heslop HE, Dotti G, Brenner MK, Shpall EJ, Bollard CM "Functionally active virus-specific T cells that target CMV, adenovirus, and EBV can be expanded from naive T-cell populations in cord blood and will target a range of viral epitopes.." Blood. 2009 Aug 27;114(9):1958-67. Pubmed PMID: 19443656
- Gerdemann U, Vera JF, Rooney CM, Leen AM "Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant.." J Vis Exp. 2011;(51) Pubmed PMID: 21654628
- Ghazi A, Ashoori A, Hanley PJ, Brawley VS, Shaffer DR, Kew Y, Powell SZ, Grossman R, Grada Z, Scheurer ME, Hegde M, Leen AM, Bollard CM, Rooney CM, Heslop HE, Gottschalk S, Ahmed N "Generation of Polyclonal CMV-specific T Cells for the Adoptive Immunotherapy of Glioblastoma.." J. Immunother.. 2012 Feb;35(2):159-68. Pubmed PMID: 22306904
- Vera JF, Hoyos V, Savoldo B, Quintarelli C, Giordano Attianese GM, Leen AM, Liu H, Foster AE, Heslop HE, Rooney CM, Brenner MK, Dotti G "Genetic manipulation of tumor-specific cytotoxic T lymphocytes to restore responsiveness to IL-7.." Mol. Ther.. 2009 May;17(5):880-8. Pubmed PMID: 19259067
- Kaka AS, Shaffer DR, Hartmeier R, Leen AM, Lu A, Bear A, Rooney CM, Foster AE "Genetic modification of T cells with IL-21 enhances antigen presentation and generation of central memory tumor-specific cytotoxic T-lymphocytes.." J. Immunother.. 2009 Sep;32(7):726-36. Pubmed PMID: 19561536
- Bollard CM, Gottschalk S, Helen Huls M, Leen AM, Gee AP, Rooney CM "Good manufacturing practice-grade cytotoxic T lymphocytes specific for latent membrane proteins (LMP)-1 and LMP2 for patients with Epstein-Barr virus-associated lymphoma.." Cytotherapy. 2011 May;13(5):518-22. Pubmed PMID: 21361747
- Quintarelli C, Dotti G, Hasan ST, De Angelis B, Hoyos V, Errichiello S, Mims M, Luciano L, Shafer J, Leen AM, Heslop HE, Rooney CM, Pane F, Brenner MK, Savoldo B "High-avidity cytotoxic T lymphocytes specific for a new PRAME-derived peptide can target leukemic and leukemic-precursor cells.." Blood. 2011 Mar 24;117(12):3353-62. Pubmed PMID: 21278353
- Lindemans CA, Leen AM, Boelens JJ "How I treat adenovirus in hematopoietic stem cell transplant recipients.." Blood. 2010 Dec 16;116(25):5476-85. Pubmed PMID: 20837781
- Ramos CA, Narala N, Vyas GM, Leen AM, Gerdemann U, Sturgis EM, Anderson ML, Savoldo B, Heslop HE, Brenner MK, Rooney CM "Human Papillomavirus Type 16 E6/E7-specific Cytotoxic T Lymphocytes for Adoptive Immunotherapy of HPV-associated Malignancies.." J. Immunother.. 2013 Jan;36(1):66-76. Pubmed PMID: 23211628
- Meij P, Leen A, Rickinson AB, Verkoeijen S, Vervoort MB, Bloemena E, Middeldorp JM "Identification and prevalence of CD8(+) T-cell responses directed against Epstein-Barr virus-encoded latent membrane protein 1 and latent membrane protein 2.." Int. J. Cancer. 2002 May 1;99(1):93-9. Pubmed PMID: 11948498
- Leen AM, Christin A, Khalil M, Weiss H, Gee AP, Brenner MK, Heslop HE, Rooney CM, Bollard CM "Identification of hexon-specific CD4 and CD8 T-cell epitopes for vaccine and immunotherapy.." J. Virol.. 2008 Jan;82(1):546-54. Pubmed PMID: 17942545
- Gerdemann U, Keukens L, Keirnan JM, Katari UL, Nguyen CT, de Pagter AP, Ramos CA, Kennedy-Nasser A, Gottschalk SM, Heslop HE, Brenner MK, Rooney CM, Leen AM "Immunotherapeutic strategies to prevent and treat human herpesvirus 6 reactivation after allogeneic stem cell transplantation.." Blood. 2013 Jan 3;121(1):207-18. Pubmed PMID: 23152545
- Luke JM, Vincent JM, Du SX, Gerdemann U, Leen AM, Whalen RG, Hodgson CP, Williams JA "Improved antibiotic-free plasmid vector design by incorporation of transient expression enhancers.." Gene Ther.. 2011 Apr;18(4):334-43. Pubmed PMID: 21107439
- Leen AM, Rooney CM, Foster AE "Improving T cell therapy for cancer.." Annu. Rev. Immunol.. 2007;25:243-65. Pubmed PMID: 17129181
- Cruz CR, Gerdemann U, Leen AM, Shafer JA, Ku S, Tzou B, Horton TM, Sheehan A, Copeland A, Younes A, Rooney CM, Heslop HE, Bollard CM "Improving T-cell Therapy for Relapsed EBV-Negative Hodgkin Lymphoma by Targeting Upregulated MAGE-A4.." Clin. Cancer Res.. 2011 Nov 15;17(22):7058-66. Pubmed PMID: 21908573
- Leen AM, Myers GD, Sili U, Huls MH, Weiss H, Leung KS, Carrum G, Krance RA, Chang CC, Molldrem JJ, Gee AP, Brenner MK, Heslop HE, Rooney CM, Bollard CM "Monoculture-derived T lymphocytes specific for multiple viruses expand and produce clinically relevant effects in immunocompromised individuals.." Nat. Med.. 2006 Oct;12(10):1160-6. Pubmed PMID: 16998485
- Rooney C, Leen A "Moving Successful Virus-specific T-cell Therapy for Hematopoietic Stem Cell Recipients to Late Phase Clinical Trials.." Mol Ther Nucleic Acids. 2012;1:e55. Pubmed PMID: 23629030
- Gerdemann U, Christin AS, Vera JF, Ramos CA, Fujita Y, Liu H, Dilloo D, Heslop HE, Brenner MK, Rooney CM, Leen AM "Nucleofection of DCs to generate Multivirus-specific T cells for prevention or treatment of viral infections in the immunocompromised host.." Mol. Ther.. 2009 Sep;17(9):1616-25. Pubmed PMID: 19584818
- Pesonen S, Diaconu I, Kangasniemi L, Ranki T, Kanerva A, Pesonen SK, Gerdemann U, Leen AM, Kairemo K, Oksanen M, Haavisto E, Holm SL, Karioja-Kallio A, Kauppinen S, Partanen KP, Laasonen L, Joensuu T, Alanko T, Cerullo V, Hemminki A "Oncolytic Immunotherapy of Advanced Solid Tumors with a CD40L-Expressing Replicating Adenovirus: Assessment of Safety and Immunologic Responses in Patients.." Cancer Res.. 2012 Apr 1;72(7):1621-31. Pubmed PMID: 22323527
- Nakazawa Y, Huye LE, Salsman VS, Leen AM, Ahmed N, Rollins L, Dotti G, Gottschalk SM, Wilson MH, Rooney CM "PiggyBac-mediated Cancer Immunotherapy Using EBV-specific Cytotoxic T-cells Expressing HER2-specific Chimeric Antigen Receptor.." Mol. Ther.. 2011 Dec;19(12):2133-43. Pubmed PMID: 21772253
- Sili U, Leen AM, Vera JF, Gee AP, Huls H, Heslop HE, Bollard CM, Rooney CM "Production of good manufacturing practice-grade cytotoxic T lymphocytes specific for Epstein-Barr virus, cytomegalovirus and adenovirus to prevent or treat viral infections post-allogeneic hematopoietic stem cell transplant.." Cytotherapy. 2012 Jan;14(1):41466. Pubmed PMID: 22172091
- Alton E, Ferrari S, Griesenbach U, Aiuti A, Bachoud-Lévi AC, Blesch A, Brenner MK, Cattaneo F, Chiocca EA, Gao G, High KA, Leen AM, Lemoine NR, McNeish IA, Meneguzzi G, Peschanski M, Roncarolo MG, Strayer DS, Tuszynski MH, Waxman DJ, Wilson JM "Progress and prospects: gene therapy clinical trials (part 2).." Gene Ther.. 2007 Nov;14(22):1555-63. Pubmed PMID: 17984995
- Gerdemann U, Keirnan JM, Katari UL, Yanagisawa R, Christin AS, Huye LE, Perna SK, Ennamuri S, Gottschalk S, Brenner MK, Heslop HE, Rooney CM, Leen AM "Rapidly Generated Multivirus-specific Cytotoxic T Lymphocytes for the Prophylaxis and Treatment of Viral Infections.." Mol. Ther.. 2012 Aug;20(8):1622-32. Pubmed PMID: 22801446
- Myers GD, Bollard CM, Wu MF, Weiss H, Rooney CM, Heslop HE, Leen AM "Reconstitution of adenovirus-specific cell-mediated immunity in pediatric patients after hematopoietic stem cell transplantation.." Bone Marrow Transplant.. 2007 Jun;39(11):677-86. Pubmed PMID: 17417664
- Leen AM, Myers GD, Bollard CM, Huls MH, Sili U, Gee AP, Heslop HE, Rooney CM "T-cell immunotherapy for adenoviral infections of stem-cell transplant recipients.." Ann. N. Y. Acad. Sci.. 2005 Dec;1062:104-15. Pubmed PMID: 16461793
- Kaloyannidis P, Leen AM, Papadopoulou A "T-cell therapy: a powerful tool for the management of viral infections and relapse post hematopoietic stem cell transplantation.." Expert Rev Hematol. 2012 Oct;5(5):471-3. Pubmed PMID: 23146049
- Leen AM "T-cell therapy: the next generation.." Expert Rev Hematol. 2010 Apr;3(2):123-5. Pubmed PMID: 21083454
- Kaka AS, Foster AE, Weiss HL, Rooney CM, Leen AM "Using dendritic cell maturation and IL-12 producing capacity as markers of function: a cautionary tale.." J. Immunother.. 2008 May;31(4):359-69. Pubmed PMID: 18391760
- Naik S, Vasileiou S, Aguayo-Hiraldo P, Mukhi S, Sasa G, Martinez C, Krance RA, Gottschalk S, Leen AM "Towards functional immune monitoring in allogeneic stem cell transplant recipients: Functional immune monitoring after transplant." Biol Blood Marrow Transplant. 2020 May;26(5):911-919. Pubmed PMID: 31927102
- Moehler M, Heo J, Lee HC, Tak WY, Chao Y, Paik SW, Yim HJ, Byun KS, Baron A, Ungerechts G, Jonker D, Ruo L, Cho M, (et.al) "Vaccinia-based oncolytic immunotherapy Pexastimogene Devacirepvec in patients with advanced hepatocellular carcinoma after sorafenib failure: a randomized multicenter Phase IIb trial (TRAVERSE)." Oncoimmunology. 2019 Jun 3;8(8):161-5817. Pubmed PMID: 31413923
- Torres Chavez A, McKenna MK, Canestrari E, Dann CT, Ramos CA, Lulla P, Leen AM, Vera JF, Watanabe N "Expanding CAR T cells in human platelet lysate renders T cells with in vivo longevity. Journal for ImmunoTherapy of Cancer." J Immunother Cancer. 2019 Nov 28;7(1):330. Pubmed PMID: 31779709
- Leung, W, Workineh, A, Mukhi, S, Tzannou, I, Brenner, D, Watanabe A, Leen AM, and Lulla, P. "Evaluation of Cyclin-A1 specific T cells as a potential treatment for myeloid leukemias. Blood Advances." Blood Adv. 2020 Jan 28;4(2):387-397. Pubmed PMID: 31985805
- 7. Kalra M, Gerdemann U, Luu JD, Ngo MC, Leen AM, Louis CU, Rooney, CM, Gottschalk S. "Epstein-Barr Virus (EBV)-derived BARF1 encodes CD4- and CD8-restricted epitopes as targets for T-cell immunotherapy." Cytotherapy .. 2019 Feb 21;21(2):212-223. Pubmed PMID: 30396848
- Sukumaran S, Watanabe N, Bajgain P, Raja K, Mohammed S, Fisher WE, Brenner MK, Leen AM, Vera JF "Enhancing the Potency and Specificity of Engineered T Cells for Cancer Treatment." Cancer Discov. 2018 Aug 8;8(8):972-987. Pubmed PMID: 29880586
- Arasaratnam RJ, Tzannou I, Gray T, Aguayo-Hiraldo PI, Kuvalekar M, Naik S, Gaikwad A, Liu H, Miloh T, Vera JF, Himes RW, Munoz FM, Leen AM "Dynamics of virus-specific T cell immunity in pediatric liver transplant recipients. Am J Transplant.." Am J Transplant .. 2018 Aug;18(9):2238-2249.
- Fuery A, Leen AM, Peng R, Wong MC, Liu H, Ling PD "Asian Elephant T Cell Responses to Elephant Endotheliotropic Herpesvirus." J Virol .. 2018 Feb 26;92(6):1951-17. Pubmed PMID: 29263271
- Bajgain P, Tawinwung S, D'Elia L, Sukumaran S, Watanabe N, Hoyos V, Lulla P, Brenner MK, Leen AM, "CAR T cell therapy for breast cancer: harnessing the tumor milieu to drive T cell activation." J Immunother Cancer. 2018 May 10;6(1):34. Pubmed PMID: 29747685
- Naik S, Nicholas SK, Martinez CA, Leen AM, Hanley PJ, Gottschalk SM, Rooney CM, Hanson IC, Krance RA, Shpall EJ, Cruz CR, Amrolia P, Lucchini G, Bunin N, Heimall J, Klein OR, Gennery AR, Slatter MA, Vickers MA, Orange JS, Heslop HE, Bollard CM, Keller MD "Adoptive immunotherapy for primary immunodeficiency disorders with virus-specific T lymphocytes." J Allergy Clin Immunol. 2016 May;137(5):1498-1505. Pubmed PMID: 26920464
- Aguayo-Hiraldo PI, Arasaratnam RJ, Tzannou I, Kuvalekar M, Lulla P, Naik S, Martinez CA, Piedra PA, Vera JF, Leen AM "Characterizing the cellular immune response to Parainfluenza virus 3." J Infect Dis. 2017 Jul 15;216(2):153-161. Pubmed PMID: 28472480
- Watanabe N, Bajgain P, Sukumaran S, Heslop HE, Rooney CM, Brenner MK, Leen AM, Vera JF "Fine-tuning the CAR spacer improves T-cell potency." Oncoimmunology. 2016 Nov 8;5(12):1253-656. Pubmed PMID: 28180032
- Tzannou I, Papadopoulou A, Naik S, Leung K, Martinez CA, Ramos CA, Carrum G, Sasa G, Lulla P, Watanabe A, Kuvalekar M, Gee AP, Wu MF, Liu H, Grilley BJ, Gottschalk S, Brenner MK, Rooney CM, Heslop HE, Leen AM, Omer B "Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus Infections After Allogeneic Hematopoietic Stem-Cell Transplantation." J Clin Oncol. 2017 Nov 1;35(31):3547-3557. Pubmed PMID: 28783452
- Tzannou I, Nicholas SK, Lulla P, Aguayo-Hiraldo PI, Misra A, Martinez CA, Machado AA, Orange JS, Piedra PA, Vera JF, Leen AM "Immunologic Profiling of Human Metapneumovirus for the Development of Targeted Immunotherapy.." J Infect Dis .. 2017 Sep 15;216(6):678-687. Pubmed PMID: 28934427
- Mohammad S, Sukumaran S, Bajgain P, Watanabe N, Heslop HE, Rooney CM, Brenner MK, Fisher WE, Leen AM, Vera JF "Improving Chimeric Antigen-Receptor Modified T Cell Function by Reversing the Immunosuppressive Tumor Microenvironment of Pancreatic Cancer." Mol Ther. 2017 Jan 4;25(1):249-258. Pubmed PMID: 28129119
- Baugh KA, Tzannou I, Leen AM "Infusion of cytotoxic T lymphocytes for the treatment of viral infections in hematopoetic stem cell transplant patients." Curr Opin Infect Dis. 2018 Aug;31(4):292-300. Pubmed PMID: 29750672
- Vasileiou S, Turney AM, Kuvalekar M, Mukhi SS, Watanabe A, Lulla P, Ramos CA, Naik S, Vera JF, Tzannou I, Leen AM "Rapid generation of multivirus-specific T lymphocytes for the prevention and treatment of respiratory viral infections.." Haematologica. 2020 Jan;105(1):235-243. Pubmed PMID: 31004020
- Tanaka M, Tashiro H, Omer B, Lapteva N, Ando J, Ngo M, Mehta B, Dotti G, Kinchington PR, Leen AM, Rossig C, Rooney CM "Vaccination Targeting Native Receptors to Enhance the Function and Proliferation of Chimeric Antigen Receptor (CAR)-Modified T Cells." Clin Cancer Res. 2017 Jul 15;23(14):3499-3509. Pubmed PMID: 28183713
- Arasaratnam RJ, Leen AM "Adoptive T cell therapy for the treatment of viral infections.." Ann Transl Med .. 2015 Oct;3(18):278. Pubmed PMID: 26605324
- Tzannou I, Leen AM "Preventing stem cell transplantation-associated viral infections using T cell therapy. Immunotherapy." Immunotherapy. 2015 Aug 7;7(7):793-810. Pubmed PMID: 26250410
- Saliba RM, Rezvani K, Leen A, Jorgensen J, Shah N, Hosing C, Parmar S, Oran B, Olson A, Rondon G, Chen J, Martinez C, Hamdi A, Mehta RS, Chemaly RF, Saunders IM, Bollard CM, Shpall EJ "General and Virus-Specific Immune Cell Reconstitution after Double Cord Blood Transplantation." Biol Blood Marrow Transplant. 2015 Jul;21(7):1284-90. Pubmed PMID: 25708219
- Tzannou I, Leen AM "Accelerating immune reconstitution after hematopoietic stem cell transplantation.." Clin Transl Immunology. 2014 Feb 28;3(2) Pubmed PMID: 25505959
- Papadopoulou A, Gerdemann U, Katari UL, Tzannou I, Liu H, Martinez C, Leung K, Carrum G, Gee AP, Vera JF, Krance RA, Brenner MK, Rooney CM, Heslop HE, Leen AM "Activity of Broad-Spectrum T Cells as Treatment for AdV, EBV, CMV, BKV, and HHV6 Infections after HSCT." Sci Transl Med. 2014 Jun 25;6(242) Pubmed PMID: 24964991
- Zhou X, Di Stasi A, Tey SK, Krance RA, Martinez C, Leung KS, Durett AG, Wu MF, Liu H, Leen AM, Savoldo B, Lin YF, Grilley BJ, Gee AP, Spencer DM, Rooney CM, Heslop HE, Brenner MK, Dotti G ". Long-term outcome after haploidentical stem cell transplant and infusion of T cells expressing the inducible caspase 9 safety transgene." Blood. 2014 Jun 19;123(25):3895-905. Pubmed PMID: 24753538
- Ildefonso CJ, Kong L, Leen A, Chai SJ, Petrochelli V, Chintagumpala M, Hurwitz MY, Chevez-Barrios P, Hurwitz RL "Absence of systemic immune response to adenovectors after intraocular administration to children with retinoblastoma." Mol Ther. 2010 Oct;18(10):1885-90. Pubmed PMID: 20606647
- Leen AM, Heslop HE, Brenner MK. "Antiviral T-cell therapy." Immunol Rev. 2014 Mar;258(1):12-29. Pubmed PMID: 24517423
- Long HM, Haigh TA, Gudgeon NH, Leen AM, Tsang CW, Brooks J, Landais E, Houssaint E, Lee SP, Rickinson AB, Taylor GS "CD4+ T-cell responses to Epstein-Barr virus (EBV) latent-cycle antigens and the recognition of EBV-transformed lymphoblastoid cell lines." J Virol .. 2005 Apr;79(8):4896-907. Pubmed PMID: 15795275
- Ngo MC, Ando J, Leen AM, Ennamuri S, Lapteva N, Vera JF, Min-Venditti A, Mims MP, Heslop HE, Bollard CM, Gottschalk S, Rooney CM. "Complementation of antigen-presenting cells to generate T lymphocytes with broad target specificity." J Immunother. 2014 May;37(4):193-203. Pubmed PMID: 24714353
- 23. Sun J, Huye LE, Lapteva N, Mamonkin M, Hiregange M, Ballard B, Dakhova O, Raghavan D, Durett AG, Perna SK, Omer B, Rollins LA, Leen AM, Vera JF, Dotti G, Gee AP, Brenner MK, Myers DG, Rooney CM "Early transduction produces highly functional chimeric antigen receptor-modified virus-specific T-cells with central memory markers: a Production Assistant for Cell Therapy (PACT) translational application.." J Immunother Cancer. 2015 Feb 18;3(5) Pubmed PMID: 25734008
- Anurathapan U, Leen AM, Brenner MK, Vera JF "Engineered T cells for cancer treatment." Cytotherapy. 2014 Jun 16; Pubmed PMID: 24239105
- Bollard CM, Kuehnle I, Leen A, Rooney CM, Heslop HE "For Viral Infections Post Transplant. Biology of Blood and Marrow Transplantation." Biol Blood Marrow Transplant. 2004 Mar;10(3):143-55. Pubmed PMID: 14993880
- Oka K, Mullins CE, Kushwaha RS, Leen AM, Chan L "Gene therapy for rhesus monkeys heterozygous for LDL receptor deficiency by balloon catheter hepatic delivery of helper-dependent adenoviral vector." Gene Ther. 2015 Jan;22(1):87-95. Pubmed PMID: 25231173
- Saliba RM, Rezvani K, Leen A, Jorgensen J, Shah N, Hosing C, Parmar S, Oran B, Olson A, Rondon "General and Virus-Specific Immune Cell Reconstitution after Double Cord Blood Transplantation." Biol Blood Marrow Transplant .. 2015 Jul;21(7):1284-90. Pubmed PMID: 25708219
- Weber G, Gerdemann U, Caruana I, Savoldo B, Hensel NF, Rabin KR, Shpall EJ, Melenhorst JJ, Leen AM, Barrett AJ, Bollard CM "Generation of multi-leukemia antigen-specific T cells to enhance the graft-versus-leukemia effect after allogeneic stem cell transplant." Leukemia. 2013 Jul;27(7):1538-47. Pubmed PMID: 23528871
- Weber G, Caruana I, Rouce RH, Barrett J, Leen AM, Rabin KR, Bollard CM "Generation of tumor antigen-specific T cell lines from pediatric patients with acute lymphoblastic leukemia - implications for immunotherapy." Clin Cancer Res. 2013 Sep 15;19(18):5079-91. Pubmed PMID: 23838315
- Melenhorst, JJ, Castillo P, Hanley PJ, Keller MD, Krance RA, Margolin J, Leen AM, Heslop HE, Barrett AJ, Rooney CM, Bollard CM. "Graft versus leukemia response without graft-versus-host-disease elicited by adoptively transferred multivirus-specific T cells." Mol Ther. 2015 Jan 23;23(1):179-83. Pubmed PMID: 25266309
- Brenner MK, Gottschalk S, Leen AM, Vera JF "Is cancer gene therapy an empty suit?." Lancet Oncol. 2013 Oct;14(11) Pubmed PMID: 24079872
- Anurathapan U, Chan RC, Hindi HF, Mucharla R, Bajgain P, Hayes BC, Fisher WE, Heslop HE, Rooney CM, Brenner MK, Leen AM, Vera JF "Kinetics of Tumor Destruction by Chimeric Antigen Receptor-modified T Cells." Mol Ther. 2014 Mar;22(3):623-633. Pubmed PMID: 24213558
- Leen AM, Bollard CM, Mendizabal AM, Shpall EJ, Szabolcs P, Antin JH, Kapoor N, Pai SY, Rowley SD, Kebriaei P, Dey BR, Grilley BJ, Gee AP, Brenner MK, Rooney CM, Heslop HE. "Multicenter study of banked third party virus-specific T-cells to treat severe viral infections after hematopoietic stem cell transplantation." Blood. 2013 Jun 27;121(26):5113-23. Pubmed PMID: 23610374
- Bajgain P, Mucharla R, Wilson J, Welch D, Anurathapan U, Liang B, Lu X, Ripple K, Centanni JM, Hall C, Hsu C, Couture LA, Gupta S, Gee A, Heslop HE, Leen AM, Rooney CM, Vera JF "Optimizing the production of suspension cells using the G-Rex “M” series. Molecular Therapy — Methods & Clinical Development." Mol Ther Methods Clin Dev. 2014 May 14;1(1405) Pubmed PMID: 26015959
- 30. Leen AM, Sukumaran S, Watanabe N, Mohammed S, Keirnan J, Yanagisawa R, Anurathapan U, Rendon D, Heslop HE, Rooney CM, Brenner MK, Vera JF "Reversal of tumor immune inhibition using a chimeric cytokine receptor." Mol Ther. 2014 Jun;22(6):1211-1220. Pubmed PMID: 24732709
- Ulrike Gerdemann, Usha L. Katari, Anastasia Papadopoulou, (et al) "Safety and clinical efficacy of rapidly-generated trivirus-directed T cells as treatment for Adenovirus, EBV and CMV infections after allogeneic hematopoietic stem cell transplant." Mol Ther. 2013 Nov;21(11):2113-21. Pubmed PMID: 23783429
- Papadopoulou A, Krance RA, Allen CE, Lee D, Rooney CM, Brenner MK, Leen AM, Heslop HE. "Systemic inflammatory response syndrome after administration of unmodified T lymphocytes." Mol Ther. 2014 Jun;22(6):1134-1138. Pubmed PMID: 24651135
- Rooney CM, Leen AM, Vera JF, Heslop HE "T lymphocytes targeting native receptors." Immunol Rev. 2014 Jan;257(1):39-55.
- Bollard CM, Straathof KC, Huls MH, Leen A, Lacuesta K, Davis A, Gottschalk S, Brenner MK, Heslop HE, Rooney CM "The generation and characterization of LMP2-specific CTLs for use as adoptive transfer from patients with relapsed EBV-positive Hodgkin disease." J Immunother. 2004 Aug 27;27(4):317-27. Pubmed PMID: 15235393
Memberships
- American Society of Gene Therapy
- International Society of Cellular Therapy
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