My project focuses on discovering new potential drug targets for cancer. Previous genome-wide RNAi screens have indentified lists of potential tumor suppressor candidate genes (tsg). We took this list and used Comparative Genomic Hybridization Array data to determine if our candidate genes are located in an area that is disrupted in patients with cancer, with a heavy emphasis on breast cancer. We have narrowed down our list to 10 genes that are found to be disrupted in patients with cancer. Our plan of action is to use RNAi technology to study these candidate tsg during mammary gland development. We are currently in the process of designing sets of shRNA for each gene and identifying the best vector to test the shRNA in. We then plan to do in vivo studies by studying the loss of function phenotype in wildtype mice as well as p53 null mice.
- Joined Lab: January 2009
- Position: Graduate Student (Developmental Biology)
- Degree: BS, University of Houston, Downtown, 2005
Santorelli LA, Thompson CR, Villegas E, Svetz J, Dinh C, Parikh A, Sucgang R, Kuspa A, Strassmann, JE, Queller DC, Shaulsky G. Facultative cheater mutants reveal the genetic complexity of cooperation in social amoebae. Nature.2008 Feb 28:451(7182):1107-10.Epub 2008 Feb 13