Before RNA samples can be processed for microarray, sequencing, or any other technology platform they must be tested for quality on the Agilent 2100 Bioanalyzer, quantitated on the NanoDrop Spectrophotometer, and tested on the Qubit 2.0 Fluorometer when needed.
Examples of GARP Passing RNA Samples
The GARP recommends using a kit to isolate and extract total RNA samples. We have found that the RNeasy Kits from Qiagen work especially well. View the protocol and see actual results from samples successfully run using the Agilent 2100 Bioanalyzer. GARP also recommends extraction kits from Zymo Research, Inc. In order to prevent false-positive results in downstream applications, RNA must be treated with DNAse incorporated into the isolation or clean-up protocols.
For RNA samples isolated from FFPE tissues, please use isolation kits specially designed for RNA isolation from FFPE tissues such as Qiagen FFPE, Zymo Research FFPE or NuGEN's Prelude FFPE RNA Isolation kit.
The GARP uses two instruments to determine the quality and concentration of samples delivered for processing: Agilent 2100 Bioanalyzer and NanoDrop® Spectrophotometer.
Agilent 2100 Bioanalyzer
The Agilent 2100 Bioanalyzer uses Lab-on-a-chip technology and capillary electrophoresis to render an extremely accurate measurement of any RNA integrity. The Agilent 2100 Bioanalyzer software has been designed to be intuitive and easy to use. For more information, please see:
The second instrument is the NanoDrop® Spectrophotometer. The instrument can precisely measure sample concentration using only 1.5 µL.