Research

Instrumentation & Technology

Master
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Facility Location

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The Genetically Engineered Mouse Core is located in the Alkek Building for Biomedical Research (ABBR) Room R223 – A Mouse Barrier Facility; Alkek Building N630, N603.01 (drop-off).

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Equipment

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Major equipment includes:

  • Nikon Eclipse Te300 Microscopes with Hoffman objective lenses
  • Nikon Diaphot inverted microscopes
  • Narishige micromanipulators
  • SMZ 800 dissecting microscopes
  • SMZ 1000 dissecting microscopes
  • FemtoJet Microinjectors
  • Sutter P-97 horizontal pull
  • de Fonbrune Microfuge
  • Bio-Cool BCIII-80A00
  • CryoSafe SSBA LN2 storage units
  • Osmometer
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Embryo Microinjection

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Microinjection Setup with Narishige Manipulators
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The embryos are microinjected with the aid of an inverted microscope equipped with differential contrast optics, usually Nomarski or Hoffman objective lenses, and micromanipulators.

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Embryo Transfer

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Embryo Transfer Setup
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The success of generating genetically engineered mice is the ability to transfer the embryos that have undergone the specific manipulation to female mice and have these mice successfully gestate those embryos.

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BioCool Apparatus with Embryos Straws for Cryopreservation

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BioCool Apparatus with Embryos Straws for Cryopreservation
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Service Scheduling

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All scheduling of services from the GEM Core is done through iLab. View scheduling information.