USDA/ARS Children's Nutrition Research Center

Confocal Microscopy and Image Analysis Core

Master
Media Component
Confocal Microscopy and Image Analysis Core is available to both CNRC personnel as well as college-wide within Baylor as a fee-for-service facility.
Content

The Confocal Microscopy and Image Analysis Core provides instrumentation and expertise to support the acquisition, processing and analysis of both fluorescence and bright-field micrographs captured from fixed tissue sections, whole mounts, or from either fixed or live cells.

Digital micrographs can be captured from any one of four different microscopes including an Olympus FV300 Laser Scanning Confocal Microscope capable of collecting 3-dimensional or time series data on specimens containing up to 4 separate fluorochromes.

An image processing and analysis workstation provides tools to process and collect data from digital micrographs. The capabilities of this system include deconvolution, co-localization, 3-dimensional reconstruction, pixel-intensity measurements, and morphometry. Analysis can be conducted using both manual and automated approaches.

Custom cryosectioning, staining, image acquisition and analysis are also available.