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Genetic Labs

Houston, Texas

Genetic Laboratory
Medical Genetics Laboratories
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Medical Genetics Test Details

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T-Cell Receptor Beta Gene Rearrangement - FFPE
Test Information: The T-Cell Receptor Beta Gene Rearrangement test is based on the detection of rearrangements of the T-cell receptor genes that occur during T lymphocyte development. T-Cell Receptor Beta gene (TRB@) rearrangement generates DNA segments that are unique in length and sequence for a specific lymphocyte clone. Polymerase chain reaction (PCR) assays can be used to identify lymphocyte populations derived from a single cell (monoclonal populations) by detecting the unique V-J gene rearrangements present within this antigen receptor locus. Reactive lymphocytic proliferations show a polyclonal rearrangement profile.The identification of a monoclonal population in the right clinical and pathological scenario, suggests the presence of a lymphoid neoplasm (leukemia or lymphoma). Non-neoplastic lymphocytic proliferations can also present with monoclonal populations.
Test Details
Test Code: 9225
Special Notes: A comprehensive T-cell clonality test that combines both the T-cell Receptor Beta and Gamma gene assays is available. Please see test codes #9217 (PB, BM, T) and #9245 (FFPE).

If the cell lineage is not known, this test can be combined with the Comprehensive B-Cell Clonality Analysis for detection of clonal B-cell proliferations. Please see test codes #9202 (PB, BM, T) or #9240 (FFPE).

To order this test on Peripheral Blood, Bone Marrow or Fresh Tissue, please see test code #9200.
Technical Information
Methodology: This assay employs multiple consensus DNA primers that target conserved genetic regions within the TRB@ gene locus. The methodology used (Invivoscribe ASR reagents, VDJ recombination) is based on data produced by independent testing centers throughout Europe in a collaborative study known as the BIOMED-2 Concerted Action. Unique rearrangements products are amplified by PCR followed by size determination using capillary electrophoresis. A clonal population of cells will produced one or two prominent amplicons within the expected size range. In contrast, DNA from normal polyclonal populations will produce amplicon products that reflect the heterogeneous population of V-J rearrangements in a Gaussian distribution.
Sample & Shipping Information
Test Requisition: Tumor Analysis
Specimen Type: FFPE - Slide(s)
Requirements: 1 H&E stained slide and 10 unstained 10um thick FFPE slides (non-charged, non-coated) containing adequate amounts of tumor to be analyzed**. Air dry. Do not oven dry. Please include a copy of corresponding surgical pathology report. Store specimen at ambient temperature (18-25C/64-77F).
Shipping Conditions: Ship at ambient temperature (18-25C/64-77F) in an insulated container by overnight courier. If shipping during the summer months please include a cold-pack to avoid extreme temperatures. Do not heat or freeze.

Specimen Type: FFPE - Tumor Block
Requirements: Paraffin-embedded, formalin-fixed tissue block. Please include a copy of corresponding surgical pathology report. Store specimen at ambient temperature (18-25C/64-77F).
Shipping Conditions: Ship at ambient temperature (18-25C/64-77F) in an insulated container by overnight courier. If shipping during the summer months please include a cold-pack to avoid extreme temperatures. Do not heat or freeze.

Turn Around Time: 4 - 7 days
Billing Information
List Price: *For Insurance or Institutional Prices, please call.
CPT Codes: 81340x1


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