About the Bissig Lab

The Bissig laboratory is located in the Center for Cell and Gene Therapy at Baylor College of Medicine. The group is interested in liver disease, ranging from viral hepatitis to metabolic liver disease and liver cancer. The Bissig laboratory is specialized in human liver chimeric mice and created the first xenograft model for metabolic liver disease as well as a novel patient derived cancer model. These humanized mouse models will help to advance and improve experimental therapies for liver disease.

More recently the Bissig laboratory moved into genome editing using CRISPR/Cas9 in human liver chimeric mice and development of optimized mouse models for human liver disorders and drug metabolism.

Techniques/Methods in the Bissig Lab

  • Human hepatocyte isolation and transplantation into FRG mice
  • CRISPR/Cas9 genome engineering in TC and in vivo
  • Gene therapy/virology techniques (virus preparation, titering, infections, etc.)
  • Stem cell targeting and generation of transgenic mouse models
  • CRISPR/Cas9 genome engineering in TC and in vivo
  • Human and mouse induced pluripotent stem cell culture
  • Standard molecular biology techniques (cloning, DNA/RNA/protein work)