The Lab is fully equipped for tissue culture, electrophysiology, and functional assays with primary human and rodent cells and cell lines. We are also equipped for molecular biology and in vivo assays.

Tissue Culture

  • Biosafety level two cabinets
  • CO2 incubator
  • Inverted microscope
  • Water bath
  • Cryotanks
  • Cell harvester
  • Refrigerated and non-refrigerated centrifuges

Patch-Clamp Electrophysiology

  • Automated Nanion Port-a-Patch with a HEKA EPC10-USB amplifier
  • Heka pipet puller
  • Narishige microforge
  • Manual rig with a fluorescence Olympus IX71 microscope, a HEKA EPC10-USB amplifier, an Eppendorf micromanipulator, a CCD-cooled black and white camera, a home-built pressure/suction controller, and an air table

Biochemistry and Molecular Biology

  • Eppendorf thermocycler
  • Microcentrifuge
  • Plate reader
  • Gel scanner
  • Photometer
  • Osmometer
  • Sonicator
  • Fume hood
  • Small equipment for DNA and protein gels
  • Small equipment for reagent preparation and storage (fridges, -20ºC and -80ºC freezers, balances, pH meter, etc…)

Animal Work and Histology

  • VetEquip anesthesia system
  • Dissection binoculars
  • Cytocentrifuge
  • Olympus BX81 microscope for histology
  • Digital color camera

Other

Besides this equipment, we have access to shared equipment within the Department of Molecular Physiology and Biophysics and within the Advanced Technology Cores Labs at Baylor College of Medicine.