Pax2-Cre Transgenic Mice

These mice were generated in our lab at the House Ear Institute by Taka Ohyama. The Cre recombinase gene was inserted into a bacterial artificial chromosome (BAC) containing about 120kb of the Pax2 genomic locus by homologous recombination in bacteria.

The Genesis paper describing the construction and characterization of these mice is available.

Pax2-Cre mice are available for purchase from the Mutant Mouse Regional Resource Center in North Carolina.

A generic Cre genotyping protocol for the mice is available. A more specific genotyping protocol that identifies the IRES-Cre element specifically is also available, but bear in mind that this protocol will detect other IRES-Cre transgenes too.

We and others have observed that the Pax2-Cre transgene can be active in the female germline. There have been isolated reports of Pax2 being expressed in female gonads, which may explain this result.  Using Pax2-Cre females to create conditional mutants can therefore give embryos with recombination throughout the body. This effect seems to have variable penetrance depending on the background strains of the mice and the floxed allele involved. For this reason, we recommend that only Pax2-Cre males are used to generate conditional mutants

Foxi3 Mutant Mice

Foxi3 mutant mice were generated in our lab at the House Ear Institute by Taka Ohyama. The loxP-flanked allele can be turned into a null allele by mating with Cre-expressing lines, which removes exon 2. 

Foxi3-flox mice are available for purchase from the Jackson Laboratory, here.

A genotyping protocol for the mice can be found here.

Shirokova, V., Jussila, M., Biggs, L.C., Ohyama, T., Groves, A.K. and Mikkola, M.L. (2016). Foxi3 deficiency compromises hair follicle stem cell specification and activation. Stem Cells 10.1002/stem.2363

Birol, O., Ohyama, T., Edlund, R.K., Drakou, K., Georgiades, P., and Groves, A.K. (2015). The mouse Foxi3 transcription factor is necessary for the development of posterior placodes. Developmental Biology 409, 139-151.

Jussila, M., Aalto, A., Sanz Navarro, M., Shirokova, V., Kallonen, A., Ohyama, T., Groves, A.K., Mikkola, M.L. and Thesleff, I. (2015). Suppression of epithelial differentiation by Foxi3 is essential for molar crown patterning. Development 142, 3954-3963.

Edlund, R.K., Ohyama, T., Kantarci, H., Riley, B.B. and Groves, A.K. (2014). Foxi transcription factors promote pharyngeal arch development by regulating the formation of FGF signaling centers. Developmental Biology 390, 1-13.