Histology and Biochemistry

Joanna Jankowsky Lab

Master

Media Component

Thioflavine plaque

Heading

Triple Labeling for Amyloid, Microglia, and a Therapeutic Anti-Aβ Antibody

Content

Thioflavine-S staining similar to that shown here was used by student Angie Chiang to measure fibrillar amyloid before and after treatment in our APP transgenic mice. Related Link: Genetic modulation of soluble Aβ rescues cognitive and synaptic impairment in a mouse model of Alzheimer’s disease

Heading

General Use

Content

Buffers and broths

Dox dosage calculations

Paraformaldehyde for tissue fixation

Transcardial perfusion

Heading

Biochemistry

Content

Diethylamine extraction for soluble Aß measurement from Liz Eckman

Formic acid extraction for total Aß measurement from Linda and Steve Younkin

Immunoprecipitation of brain proteins

Proteinase K tissue DNA extraction

SDS/Formic acid extraction for Aß measurement from Linda and Steve Younkin

TBS / TBS-X / Guanidine extraction for Aß measurement from Guojun Bu

Western blotting for brain proteins

Heading

Histology

Content

Aβ immunohistochemistry

Antibodies used by the lab

Campbell-Switzer silver stain

Congo Red stain

Cresyl violet staining

Fractin immunostain

Gallyas silver stain

Gelatin embedding of fixed tissue

Golgi staining with FD Rapid Golgi kit

Hematoxylin and eosin staining

Immunocytochemistry of cultured cells

Immunohistochemistry with free-floating sections

Nuclear fast red stain

Perls Prussian blue stain

RNase pretreatment for nuclear cresyl violet stain

Thioflavine-S staining for amyloid deposits

Timm’s staining

von Kossa calcium stain

Heading

Cell Culture

Content

HEK cell maintenance

N2a cell maintenance