About the Core
The Core provides a full service that includes dissecting and collecting animal tissue specimens, preparation of frozen sections, preparation of RNA probes from customer templates, conducting high-throughput ISH1, whole-slide microscopy (brightfield or fluorescence) and quantification of expression patterns. We also offer X-gal staining of sections.
1Yaylaoglu MB, Titmus A, Visel A, Alvarez-Bolado G, Thaller C, Eichele G, Dev Dyn. 2005 Oct; 234(2):371-86.

Services Available
The following services are available:
- Rodent dissection and tissue embedding
- Training for dissection and tissue embedding
- Tissue sectioning (cryostat)
- mRNA probe preparation
- RNA in situ hybridization (chromogenic or fluorescent)
- X-gal staining
- Whole section imaging (mosaic images), bright field or fluorescent
- Quantification of RNA in situ hybridization signal (brightfield only)
Click here for more information.
Information for Users
To discuss project details, pricing and scheduling please contact Dr. Cecilia Ljungberg (cecilial@bcm.edu). Here is the current price list for IDDRC members. Volume discounts are available.
All services requests are processed through iLabs:
Recent Publications Using the Core
- White JJ, Sillitoe RV. Genetic silencing of olivocerebellar synapses causes dystonia-like behavior in mice. Nat. Commun. 2017; 8: 14912. PMID: 28374839, PMCID: PMC5382291
- Tischfield MA, Robson CD...Engle EC. Cerebral Vein Malformations Result from Loss of Twist1 Expression and BMP Signaling from Skull Progenitor Cells and Dura. Dev Cell. 2017, 42(5):445-461.e5. doi: 10.1016/j.devcel.2017.07.027. PMID: 28844842
- Tan Q, Yalamanchili HK, Park J, De Maio A, Lu HC, Wan YW... Zoghbi HY. Extensive cryptic splicing upon loss of RBM17 and TDP43 in neurodegeneration models. Hum. Mol. Genet. 2016, pii: ddw337. doi: 10.1093/hmg/ddw337. PMID: 28007900.
- Palmieri M, Pal R, Nelvagal HR, Lotfi P,...Sardiello M. mTORC1-independent TFEB activation via Akt inhibition promotes cellular clearance in neurodegenerative storage diseases. Nat. Commun. 2017; 8: 14338. PMID: 28165011, PMCID: PMC5303831
- Nugent AA, Park JG, Wei Y, Tenney AP, Gilette NM...Engle EC. Mutant α2-chimaerin signals via bidirectional ephrin pathways in Duane retraction syndrome. J Clin Invest. 2017, 127(5):1664-1682. doi: 10.1172/JCI88502. PMID: 28346224
- Mulherkar S, Firozi K, Huang W...Tolias, K. RhoA-ROCK Inhibition Reverses Synaptic Remodeling and Motor and Cognitive Deficits Caused by Traumatic Brain Injury. Scientific Reports 2017 (7) 10689, doi: 10.1038/s41598-017-11113-3. PMID: 28878396
- Lu H-C, Tan Q, Rousseaux MWC, Wang W,...Schaaf CP, Zoghbi HY. Disruption of the ATXN1-CIC complex causes a spectrum of neurobehavioral phenotypes in mice and humans. Nature Genet. 2017, 49(4): 527–536. PMID: 28288114, PMCID: PMC5374026
- Bomben VC, Aiba I, Qian J, Mark MD, Herlitze S and Noebels JL. Isolated P/Q calcium channel deletion in Layer VI corticothalamic neurons generates absence epilepsy. J. Neurosci. 2016; 36:405-418. PMID: 26758833 PMCID: PMC4710767.
- Dharmadhikari AV, Sun JJ, Gogolewski K, Carofino BL, Ustiyan V, Hill M,...Stankiewicz P. Lethal lung hypoplasia and vascular defects in mice with conditional Foxf1 overexpression. Biol Open. 2016; 5:1595-1606. PMID: 27638768 PMCID: PMC5155529.
- Lian H, Litvinchuk A, Chiang AC,...Zheng H. Astrocyte-Microglia Cross Talk through Complement Activation Modulates Amyloid Pathology in Mouse Models of Alzheimer's Disease. J. Neurosci. 2016; 36:577–589. PMID: 26758846 PMCID: PMC4710776.
- Maass JC,...Groves AK. Transcriptomic Analysis of Mouse Cochlear Supporting Cell Maturation Reveals Large-Scale Changes in Notch Responsiveness Prior to the Onset of Hearing. PLoS One. 2016;11:e0167286. PMID: 27918591
Acknowledgement
We ask that all IDDRC users acknowledge the core as follows:
"The project described was supported in part by the RNA In Situ Hybridization Core facility at Baylor College of Medicine, which is supported by a Shared Instrumentation grant from the NIH (1S10OD016167) and the NIH IDDRC grant U54HD083092 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health & Human Development or the National Institutes of Health".
A copy of your publication would also be greatly appreciated.