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RNA In Situ Hybridization

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About the Core

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The Core provides a full service that includes dissecting and collecting animal tissue specimens, preparation of frozen sections, preparation of RNA probes from customer templates, conducting high-throughput ISH1, whole-slide microscopy (brightfield or fluorescence) and quantification of expression patterns. We also offer X-gal staining of sections.

1Yaylaoglu MB, Titmus A, Visel A, Alvarez-Bolado G, Thaller C, Eichele G, Dev Dyn. 2005 Oct; 234(2):371-86.

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Services Available

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The following services are available:

  • Rodent dissection and tissue embedding
  • Training for dissection and tissue embedding
  • Tissue sectioning (cryostat)
  • mRNA probe preparation
  • RNA in situ hybridization (chromogenic or fluorescent)
  • X-gal staining
  • Whole section imaging (mosaic images), bright field or fluorescent
  • Quantification of RNA in situ hybridization signal (brightfield only)

Click here for more information.

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Information for Users

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To discuss project details, pricing and scheduling please contact Dr. Cecilia Ljungberg (cecilial@bcm.edu). Here is the current price list for IDDRC members. Volume discounts are available.

All services requests are processed through iLabs:

Create a new iLabs account.

Log in to an existing iLabs account.

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Recent Publications Using the Core

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Acknowledgement

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We ask that all IDDRC users acknowledge the core as follows:

"The project described was supported in part by the RNA In Situ Hybridization Core facility at Baylor College of Medicine, which is supported by a Shared Instrumentation grant from the NIH (1S10OD016167) and the NIH IDDRC grant U54HD083092 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health & Human Development or the National Institutes of Health".

A copy of your publication would also be greatly appreciated.