Dolores J. Lamb, Ph.D.
Departments of Urology and Molecular & Cellular Biology
Ph.D., The University of Texas Graduate School of Biomedical Sciences
Postdoctoral, Baylor College of Medicine
Current funded research studies in my laboratory focus upon the the molecular basis for male reproductive defects. We have just renewed a large program project grant to examine the genetic basis for male infertility. Studies focus on genomic instability and testicular failure in azoospermia and the consequences of these defects for the offspring conceived with the assisted reproductive technologies. Our goal is to define the molecular basis for developmental and functional defects leading to male infertility. We hope to one day use this information in a dynamic and, perhaps, therapeutic role for the male factor patient.
Transplantation of adult spermatogonial stem cells may provide one route to restoration of fertility for patients rendered sterile by chemotherapy or occupational or environmental exposure to reproductive toxins. Current research is focused on the identification and purification of adult spermatogenial stem cells and development of new methods to enhance germ cell transplantation and stem cell proliferation in vivo and in vitro. Recently, we identified Leydig stem cells present in the adult testis, as well.
Matzuk MM and Lamb DJ. (2002). Genetic Dissection of Mammalian Fertility Pathways. (Invited Review). Nat Cell Biol. S1:41-9. Nature Medicine 8 (S1). S41-S49.
Maduro MR, Casella R, Kim ED, Levy N, deLara J, Lipshultz LI, Lamb DJ. (2003). Microsatellite Instability and Defects in Mismatch Repair Proteins: A New Etiology for Sertoli cell only Syndrome. Molecular Human Reproduction. 9(2):61-8.
Miyamoto T, Hasuike S, Yogev L, Maduro MR, Ishikawa M, Westphal H & Lamb DJ (2003) Azoospermia in patients heterozygous for a mutation in SYCP3. Lancet Nov 22;362(9397):1714-92003
Lo KC, Lei Z, Rao CV, Beck J, Lamb DJ. (2004) De novo testosterone production in luteinizing hormone receptor knockout mice after transplantation of Leydig stem cells. Endocrinology 145(9):4011-4015.
King SR, Ginsberg SD, Ishii T, Smith RG, Parker KL, Lamb DJ (2004) Steroidogenic acute regulatory protein is expressed in steroidogenic cells of the day-old brain. Endocrinology 145(10):4775-4780.
Lo KC, Brugh VM III, Parker M, Lamb DJ. (2005) Isolation and Enrichment of Murine Spermatogonial Stem Cells Using Rhodamine 123 Mitochondrial Dye. BiolReprod 72, 767-771.