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  • Core A |Core Facilities | Core C

    CORE B:  Cellular & Molecular Morphology Core Services

    Milton J. Finegold, M.D., Director
    Michael Mancini, Ph.D., Associate-Director
    Cecilia Ljungberg, Ph.D., Associate Director
    Pamela Parsons :  Laboratory Manager

    Services

    CORE B-1
    A. Routine histology - processing/embedding, grossing/submitting, H&E, Microtomy, Special stains
    (simple and complex), Frozen block preparation (freezing tissue in OCT), Cut unstained section from frozen block
    B. Immunohistochemistry; IHC staining, Antibody Titration, Tunel staining
    C. Enzyme histochemistry
    D. Immunofluorescence (frozen sections or cells)
    E. Electron microscopy - complete processing, scanning; sample prep (critical point drying), use of scope
    (assisted/unassisted), and immuno-electron microscopy
    F. Laser Capture Microscopy (LCM) consultation, training and supervision for investigator use
    G. Serum chemistries - Liver battery (albumin, aspartate aminotransferases, alanine aminotransferases
    alkaline phosphatase, gamma-glutamyl transpeptidase, total bilirubin, conjugated bilirubin)
    Other assays and services by arrangement with Dr. Devaraj

    CORE B-2
    H. Transmission Electron microscopy, sample preparation, processing and instrument use
    I. Image Analysis Workstation
    J. Upright Brightfield and Epifluorescence Inverted Microscopes
    K. Confocal Microscope (live or fixed cells)
    L. Deconvolution Microscope (live or fixed cells)
    M. High Throughput Microscope (live or fixed cells)
    N. Consultation and technical advice to researchers interested in developing sophisticated procedures for their GI-related projects (assay development and microscopy). Technical Assistance/Training (instrument use, immunofluorescence and RNA in situ hybridization)
    O. Biomek NX Robot (tips and supplies not included)

    CORE B-3
    P. Tissue processing, embedding and sectioning
    Q. Tissue sectioning
    R. Probe preparation - mRNA probe preparation / probe from core stock
    S. In situ hybridization
    T. Scanning (automated mosaic images)
    U. Data Analysis - cell detection and counting

    Forms

    Morphology CORE B-1 Research Request
    Morphology CORE B-1 Charge List
    Research Request for Laser Capture Microscopy
    Microscopy CORE B-2 Charge List
    RNA In situ Hybridization CORE B-3 Charge List

     

    Core A |Core Facilities | Core C