Mouse Metabolism Core
The Mouse Metabolism Core facility under DRC is one out of 11 NIH-funded core facilities throughout the United States that provides research support to the investigators. This core serves new and established investigators working in the field of diabetes and metabolic diseases. The core also offers extensive support to other investigators wishing to address diabetes-related hypotheses in terms of metabolic disorders and diabetes utilizing mouse models.
The core is located at the 6th floor of the Margaret Alkek Building for Biomedical Research, Room R630. Hours of operation are 9:30 a.m. to 5 p.m. Services provided by the Mouse Metabolism Core include:
- Cellular oxygen consumption and glycolysis monitoring using extracellular flux analyzer (XF24) from Seahorse Bio.: Measure mitochondrial respiration in intact skeletal muscle fibers, Fatty Acid Oxidation in C2C12 myocytes, glycolysis and oxidative metabolism in cancer cells, understand the relationship between bioenergetic rates, ATP turnover and more
- Comprehensive Laboratory Animal monitoring system (CLAMS): Simultaneous monitoring of Food intake,Energy expenditure and real-time body temperature (surgery required for temp.) by measuring VO2, VCO2, RQ (respiratory quotient) and heat production
- Euglycemic Hyperinsulinemic Clamp: Hepatic glucose production, glucose disposal rate, glucose infusion rate, glycogen synthesis and tissue specific glucose uptake using conscious mouse
- Long term infusion through jugular vein in non-sedated mouse for metabolic studies
- MRI: Body composition analysis of conscious mice
- Mouse activity monitoring: Total activity including horizontal activity, distance travel, rest time by beam block technique in home cage
- GTT: Intra peritoneal Glucose tolerance test and oral GTT
- ITT: Insulin Tolerance test
- In vivo lipolysis
- Lipid profile measurement: VLDL, HDL, LDL
- Routine blood parameter check: Includes glucose, insulin, cholesterol, leptin, adiponectin, TG, free fatty acid, glycerol
- Freshly isolated primary hepatocytes
- Freshly isolated islets
- Lipid Extraction from various tissue
- TLC run for lipid assay
The core also provides help in data analysis and scientific explanation with proper discussion related to the metabolic changes.
**Discounts are offered to BCM faculty or investigators with NIH funding. For-profit corporations, please call for pricing.
- Dr. Pradip Saha, Co-Director
- Luge Li, Technician
- Leslie Wu, Administrator