Lauren C. Goldie, Ph.D.
Instructor, Baylor College of Medicine
B.Sc.(Hons) – University of Western Australia, Perth, Australia
Ph.D. – University of Western Australia, Perth, Australia
Postdoctoral – Baylor College of Medicine, Houston TX
Developmental Vascular Biology and Hematopoiesis
My research broadly seeks to understand the molecular and cellular mechanisms which regulate blood and blood vessel formation during embryonic development. Of particular interest is the relative role of intrinsic molecular regulation versus extrinsically-dictated microenvironmental cues which contribute to the establishment of diverse endothelial cell phenotypes and functions. Current work is focused on elucidating intrinsic molecular regulatory pathways which govern the phenotypic and functional specialization of vascular endothelial cells from human embryonic stem cells and tissue-specific multipotent progenitors. Major long-term goals are to determine whether tissue-of-origin dictates endothelial cell identity, and how endothelial cells of differing tissue origins contribute to developmental vasculogenesis and adult neovascularization.
A second major research focus is the molecular regulation of vascular endothelial and blood cell development from bipotent mesodermal progenitors. The long-term goal of this work is to elucidate molecular signals mediating the differentiation of hemogenic (“blood-forming”) endothelium from non-hematopoietic vascular endothelium during embryogenesis. Current studies are focused on the role of a known regulator of adult hematopoiesis, retinoic acid (RA), in the emergence of specialized hemogenic endothelium, which is critical for establishment of the definitive hematopoietic stem cell compartment during normal ontogeny. In addition to aiding our understanding of the molecular events which regulate developmental hematopoiesis, these studies provide much-needed insight into the developmental potential of mesodermal progenitors that reside within adult tissues such as bone marrow, liver and skeletal muscle. Learning how to harness the potential of multipotent adult progenitors such as these is crucial for the development of improved vascular regenerative therapies with relevant human clinical application.
Goldie, L.C., Kelly, M.A. and Hirschi, K.K. (2010) A natural history of the endothelial cell: from specification to specialization (Review). Trends in Developmental Biology (In press).
Karimi, M., Goldie, L.C., Cruickshank, M., Moses, E.K. and Abraham, L.J. (2009) A critical assessment of the factors affecting reporter gene assays for promoter SNP function: a reassessment of -308 TNF polymorphism function using a novel integrated reporter system. European Journal of Human Genetics 17:1454-1462.
Goldie, L.C., Lucitti, J., Dickinson, M.E. and Hirschi, K.K. (2008) Cell signaling directing the formation and function of hemogenic endothelium during murine embryogenesis. Blood 112(8):3194-3204.
Goldie, L.C., Kelly, M.A. and Hirschi, K.K. (2007) Embryonic vasculogenesis and hematopoietic specification. In: C. Ruhrberg (Editor), VEGF in Development (pp 40-51). Landes Bioscience and Springer Science+Business Media, Austin TX, USA, ISBN:0387786317.