Douglas G. Burrin, Ph.D.
Professor of Pediatrics, Baylor College of Medicine
Research Physiologist, Agricultural Research Service
1100 Bates Street, Suite 10068
Houston, Texas, USA 77030-2600
Lab web site:
Other Academic Appointments
Director, Fellowship Research Training, Section of Pediatric Gastroenterology, Hepatology, and Nutrition, Baylor College of Medicine
BS Animal Science, Purdue University
MS Animal Science, University of Nebraska
Ph.D. Nutrition and Physiology, University of Nebraska
Postdoctoral Fellowship, Pediatric Nutrition, Baylor College of Medicine
Nutritional Regulation of Neonatal Gut and Liver Health and Disease
My laboratory has several basic and translational projects designed to establish how nutritional support, enteral versus parenteral, effects gut and liver function and susceptibility to disease in early development. We have used the neonatal piglet to established unique models of parenteral nutrition-associated liver disease (PNALD), necrotizing enterocolitis (NEC) and short-bowel syndrome (SBS) to address clinically-relevant problems in pediatric gastroenterology.
Current projects in the laboratory seek to identify the cellular and molecular mechanism that lead to PNALD and metabolic dysfunction associated with prematurity and neonatal parenteral nutrition (PN) support. Our recent studies show that chronic PN induces hepatic steatosis, cholestasis and insulin resistance in term and premature neonatal piglets. We are currently exploring how specific nutrients in commercial lipid emulsions alter the susceptibility to PNALD. We are exploring how nutrients affect interorgan and local cellular signaling pathways involved in hepatic lipid metabolism and bile acid homeostasis. We are also testing whether the adverse metabolic phenotype induced by PN is programmed and persists beyond the neonatal period and predisposes to adolescent fatty liver disease and type 2 diabetes.
Studies also are aimed at establishing the cellular and physiological functions of glucagon-like peptide 2 (GLP-2), an FDA-approved gut hormone currently in clinical trial for treatment of adult short-bowel syndrome. Past studies in TPN-fed piglets were first to show the trophic and vasoactive actions of GLP-2 in the neonatal gut. We identified the cellular co-localization of the GLP-2 receptor in enteric neurons with neurotransmitters. Current studies are aimed at establishing unique enterally-mediated signaling mechanisms that trigger enteroendocrine cell GLP-2 secretion and GLP-2 receptor function. We are also testing the efficacy of GLP-2 administration for prevention of NEC and treatment of SBS in premature piglet models.
We take an integrative experimental approach dictated by the research question to address relevant functions at the whole animal, tissue, cellular or molecular level. We use sophisticated metabolic, cell biological and molecular approaches, such as stable isotope metabolomics, laser-capture microdissection, gene microarray, and confocal microscopic imaging to identify the cellular localization of specific signals involved in the metabolism, proliferation and survival of relevant cell types, including mucosal epithelial cells and hepatocytes.
Benight, NM, B Stoll, O Olutoye, JJ Holst, DG Burrin. GLP-2 delays but does not prevent the onset of necrotizing enterocolitis in preterm pigs. J Ped Nutr Gastro. 2013 [Epub ahead of print]
Bauchart-Thevret, C, B Stoll, NM Benight, O Olutoye, D Lazar, DG Burrin. Supplementing monosodium glutamate to partial enteral nutrition slows gastric emptying in preterm pigs. J. Nutr. 2013 [Epub ahead of print]
Puiman, P, B Stoll, L Mølbak, A de Bruijn, H Schierbeek, M Boye, G Boehm, I Renes, J van Goudoever, D Burrin. Modulation of the gut microbiota with antibiotic treatment suppresses whole body urea production but not threonine oxidation in neonatal pigs. Am J Physiol. 304:G300-310, 2013.
Marini, JC, B Stoll, IC Didelija, DG Burrin. De novo synthesis is the main source of ornithine for citrulline production in neonatal pigs. Am J Physiol. 303:E1348-1353, 2012.
Jain, AK, B Stoll, DG Burrin, JJ Holst, DD Moore. Enteral bile acid treatment improves parenteral nutrition related liver disease and intestinal mucosal atrophy in neonatal piglets. Am J Physiol. 302: G218–G224, 2012.
Stoll, B, PJ Puiman, L Cui, X Chang, NM Benight, C Bauchart-Thevret, B Hartman, JJ Holst, DG Burrin. Continuous parenteral and enteral nutrition induces metabolic dysfunction in neonatal pigs. JPEN, 36:538-550, 2012.
Benight, NM, B Stoll, JC Marini, DG Burrin. Oral methylthioadenosine supplementation is anti-inflammatory and prevents DSS-induced colitis in mice. Am J Physiol. 303:G71-82, 2012.
Gay AN, D Lazar, B Stoll, B Naik, DG Burrin, O Olutoye. Near-infrared spectroscopy measurement of abdominal tissue oxygenation as an indicator of intestinal blood flow and necrotizing enterocolitis in premature piglets. J. Pediatr Surg. 46:1034-1040, 2011.
Benight, NM, B Stoll, S Chacko, VR da Silva, JC Marini, JF Gregory, SP Stabler, DG Burrin. B-vitamin Deficiency is Protective Against DSS-Induced Colitis in Mice. Am. J. Physiol. 301:249-259, 2011.
Puiman, PJ, B Stoll, JB van Goudoever, DG Burrin. Enteral arginine does not increase superior mesenteric arterial blood flow but induces mucosal growth in neonatal pigs. J. Nutr. 141:63-70, 2011.
Stoll, B, D Horst, L Cui, X Chang, K Ellis, DL Hadsell, A Suryawan, A Kurundkar, A Maheshwari, TA Davis, DG Burrin. Chronic parenteral nutrition induces hepatic inflammation, steatosis and insulin resistance in neonatal piglets. J. Nutr. 140:2193-2200, 2010.
Bauchart-Thevret C, L Cui, G Wu, B Stoll, D Burrin. Arginine-induced stimulation of protein synthesis and survival in IPEC-J2 cells is mediated by mTOR but not nitric oxide. Am J Physiol, 299:E899-909, 2010.
Thymann, T, B Stoll, HK Møller, AC Stoy, RK Buddington, S Bering, BB Jensen, OO Olutoye, RH Siggers, L Mølbak, PT Sangild, DG Burrin. Carbohydrate maldigestion induces necrotizing enterocolitis in preterm pigs. Am J Physiol. 297:G1115-1125, 2009.
Bauchart-Thevret, C., B Stoll, S Chacko and DG. Burrin. Sulfur amino acid deficiency upregulates intestinal methionine cycle activity and suppresses epithelial growth in neonatal pigs. Am J Physiol. 296:E1239-1250, 2009.
Riedijk, MA, B Stoll, S Chako, H Schierbeek, AL Sunehag, JB van Goudoever, DG Burrin. Methionine transmethylation and transsulfuration in the piglet gastrointestinal tract. Proc Natl Acad Sci. 104:3408-3413, 2007.
Guan, X, HE Karpen, JT Bukowski, J Stephens, S Niu, G Zhang, B Stoll, , MJ Finegold, JJ Holst, DL Hadsell, BL Nichols, and DG Burrin. GLP-2 receptor localizes to enteric neurons and endocrine cells expressing vasoactive peptides and mediates increased blood flow. Gastroenterology. 130:150-164, 2006.
Sangild, PT, RH Siggers, M Schmidt, J Elnif, CR Bjornvad, T Thymann, ML Grondahl, AK Hansen, SK Jensen, M Boye, L Moelbak, RK Buddington, B Westrom, DG Burrin. A diet- and colonization-dependent intestinal dysfunction predisposes to necrotizing enterocolitis (NEC) in preterm pigs. Gastroenterology 130:1776-1792, 2006.